06-1049 | Anti-Axin-1 Antibody

06-1049
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, ICC, IHCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number06-1049
      DescriptionAnti-Axin-1 Antibody
      Alternate Names
      • Axin-1
      • Axis inhibition protein 1
      • hAxin
      Background InformationAxin-1 is a beta-catenin destruction complex scaffold protein and a transcription factor of the Wnt signaling pathway. Variation in the Wnt signaling pathway has been associated with the formation of some primary human cancers. Axin-1 mutation has been linked to oral squamous cell carcinoma. Axin-1 binds to GSK3alpha and activates the Wnt signaling pathway which is shown to be associated with growth and development of cells. Axin-1 has also been implicated in Alzheimer's disease.
      References
      Product Information
      FormatAffinity Purified
      Control
      • HEK293 cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Axin-1 Antibody is an antibody against Axin-1 for use in WB, IC, IH.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesImmunocytochemistry Analysis: 1:500 dilution from a representative lot detected Axin-1 in A431 and HeLa cells.

      Immunohistochemistry Analysis: 1:1,000 dilution from a representative lot detected Axin-1 in human kidney and human skin tissues.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human Axin-1.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain. The encoded protein interacts with adenomatosis polyposis coli, catenin beta-1, glycogen synthase kinase 3 beta, protein phosphate 2, and itself. This protein functions as a negative regulator of the wingless-type MMTV integration site family, member 1 (WNT) signaling pathway and can induce apoptosis. The crystal structure of a portion of this protein, alone and in a complex with other proteins, has been resolved. Mutations in this gene have been associated with hepatocellular carcinoma, hepatoblastomas, ovarian endometriod adenocarcinomas, and medullablastomas. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq].
      Gene Symbol
      • AXIN1
      • AXIN
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Component of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling. Controls dorsoventral patterning via two opposing effects; down-regulates CTNNB1 to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway. In Wnt signaling, probably facilitates the phosphorylation of CTNNB1 and APC by GSK3B. Likely to function as a tumor suppressor. Facilitates the phosphorylation of TP53 by HIPK2 upon ultraviolet irradiation. Enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7. Also component of the AXIN1-HIPK2-TP53 complex which controls cell growth, apoptosis and development.

      SUBUNIT STRUCTURE: Homodimer (By similarity). Component of the beta-catenin destruction complex, containing at least, CTNNB1, an axin and GSK3B, that regulates CTNNB1 protein levels through phosphorylation and ubiquitination. Interacts with CTNNB1 (via the armadillo repeats 2-7). Interacts with GSK3B; the interaction hyperphosphorylates CTNNB1 leading to its ubiquitination and destruction. Component of the AXIN1-HIPK2-TP53 complex. Interacts directly in the complex with TP53 and HIPK2. Interacts with DAXX; the interaction stimulates the interaction of DAXX with TP53, stimulates 'Ser-46' phosphorylation of TP53 and induces cell death on UV irradiation. Also binds APC, SMAD6, SMAD7 and RNF111. Interacts with DIXDC1; prevents interaction with MAP3K1. Interacts with MAP3K4. Interacts with ANKRD6 and AIDA (By similarity). Interacts with MDFI; the interaction decreases AXIN1-mediated JUN N-terminal kinase (JNK) activation. Interacts with MDFIC; the interaction inhibits beta-cateninin-mediated signaling and AXIN1-mediated JUN N-terminal kinase (JNK) activation. Interacts with LRP5 (via its phosphorylated PPPSP motifs); the interaction is stimulated by WNT1 and GSK3B and activates beta-catenin signaling. Interacts (via the C-terminal) with PPP1CA; the interaction dephosphorylates AXIN1 and regulates interaction with GSK3B. Interacts with PPP2CA; the interaction dephosphorylates AXIN1.

      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note: On UV irradiation, translocates to the nucleus and colocalizes with DAAX.

      TISSUE SPECIFICTY: Ubiquitously expressed.

      PTM: Phosphorylation and dephosphorylation of AXIN1 regulates assembly and function of the beta-catenin complex. Phosphorylated by CK1 and GSK3B. Dephosphorylated by PPP1CA and PPP2CA. Phosphorylation by CK1 enhances binding of GSK3B to AXIN1.

      Sumoylation is required for AXIN1-mediated JNK activation (By similarity).

      INVOLVEMENT IN DISEASE: Defects in AXIN1 are involved in hepatocellular carcinoma (HCC) [MIM:114550].

      Defects in AXIN1 are a cause of caudal duplication anomaly (CADUA) [MIM:607864]. Caudal duplication anomaly is characterized by the occurrence of duplications of different organs in the caudal region. Note=Caudal duplication anomaly is associated with hypermethylation of the AXIN1 promoter.

      SEQUENCE SIMILARITIES: Contains 1 DIX domain.

      Contains 1 RGS domain.

      SEQUENCE CAUTION: The sequence AAC51624.1 differs from that shown. Reason: Erroneous initiation.
      Molecular Weight~110 kDa observed. An uncharacterized band appears at ~40 and 45 kDa in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HEK293 cell lysate.

      Western Blot Analysis: 1 µg/mL of this antibody detected Axin-1 on 10 µg of HEK293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies