05-1094 | Anti-B-Raf Antibody, clone 4B3.2

100 µg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMPurifiedMonoclonal Antibody
      Catalogue Number05-1094
      DescriptionAnti-B-Raf Antibody, clone 4B3.2
      Alternate Names
      • v-raf murine sarcoma viral oncogene homolog B1
      • murine sarcoma viral (v-raf) oncogene homolog B1
      • 94 kDa B-raf protein
      • B-Raf proto-oncogene serine/threonine-protein kinase (p94)
      Background InformationThe Raf proteins (Raf-1, A-Raf, B-Raf) are Ser/Thr kinases with homology to the PKC family, containing an N-terminal regulatory domain and a C-terminal catalytic domain. Members of the Raf family bind to activated Ras GTPase, which results in Raf translocation to the plasma membrane and activation. Activated Raf proteins phosphorylate MEKs, and are therefore the principle transducers of signals from Ras to MAP kinase. B-Raf acts as a signal transducer from membrane-associated receptors to nuclear transcription factors. B-Raf is important for the regulation of cell proliferation and determination of cell fate during embryogenesis. B-Raf acts downstream of Ras and upstream of MEK in the Ras-Raf-MEK-ERK signal transduction pathway, which is a conserved RAS-activated protein kinase cascade that regulates cell growth, proliferation, and differentiation in response to growth factors, cytokines, and hormones.
      Product Information
      • K562 cell lysate
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationDetect B-Raf using this Anti-B-Raf Antibody, clone 4B3.2 validated for use in WB & IC.
      Key Applications
      • Western Blotting
      Application NotesSnap I.D. Analysis: 2 µg/mL of this antibody detected B-Raf on 10 µg of K562 cell lysate.

      Immunocytochemistry Analysis: 1:500 dilution of this antibody has been shown to detect B-Raf in A431 cells.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to human B-Raf.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody recognizes B-Raf.
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq].
      Gene Symbol
      • BRAF1
      • RAFB1
      • B-Raf
      • B-RAF1
      • p943
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.

      COFACTOR: Binds 2 zinc ions per subunit (By similarity).

      SIZE: 766 amino acids; 84437 Da

      SUBUNIT: Interacts with RIT1 (By similarity).


      TISSUE SPECIFICITY: Brain and testis.

      DISEASE: Defects in BRAF are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio- cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant. & Defects in BRAF are involved in a wide range of cancers. & Defects in BRAF are involved in lung cancer [MIM:211980]. & Defects in BRAF are involved in non-Hodgkin lymphoma (NHL) [MIM:605027]. NHL is a cancer that starts in cells of the lymph system, which is part of the body's immune system. NHLs can occur at any age and are often marked by enlarged lymph nodes, fever and weight loss. & Defects in BRAF may be a cause of colorectal cancer (CRC) [MIM:114500].

      SIMILARITY: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily. & Contains 1 phorbol-ester/DAG-type zinc finger. & Contains 1 protein kinase domain. & Contains 1 RBD (Ras-binding) domain.
      Molecular Weight84 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in K562 cell lysate.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected B-Raf on 10 µg of K562 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information

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      Life Science Research > Antibodies and Assays > Primary Antibodies