CBL147 | Anti-BL-CAM Antibody, clone RFB4

CBL147
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFC, IF, IP, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberCBL147
      ReplacesCBL147PC
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-BL-CAM Antibody, clone RFB4
      Alternate Names
      • CD22
      References
      Product Information
      FormatPurified
      PresentationThe monoclonal is presented at a concentration of 100μg/1ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.
      Applications
      ApplicationAnti-BL-CAM Antibody, clone RFB4 detects level of BL-CAM & has been published & validated for use in FC, IF, IP, IH.
      Key Applications
      • Flow Cytometry
      • Immunofluorescence
      • Immunoprecipitation
      • Immunohistochemistry
      Application NotesIdentification of B lymphocytes and HCL cells by indirect immunofluorescence staining

      Identification of B lymphocytes and B lymphocyte progenitor cells by staining of cytoplasmic CD22 antigen using the APAAP technique

      Staining of B lymphocytes in frozen tissue sections by indirect immunoperoxidase staining

      Immunoprecipitation of CD22 antigen

      Signal transduction studies of CD22 which is phosphorylated following slgM cross-linking

      Studies on the interaction of CD22 and its ligand, CD45

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenHuman tonsil lymphocytes
      CloneRFB4
      HostMouse
      SpecificityThe CD22 antigen is a heterodimer composed of two glycoprotein chains of 130-140 kDa and is present as both a cytoplasmic and membrane antigen on B lymphocytes. Antigen expression appears at an early stage in B lymphocyte differentiation at approximately the same stage as the CD19 antigen. Surface expression of the CD22 antigen by B lymphocytes is variable. Circulating B lymphocytes are CD22 positive and follicular mantle and marginal zone B lymphocytes show strong binding of this antibody. Germinal centre B cells exhibit only weak expression of the CD22 antigen. The CD22 antigen is poorly expressed by non T ALL cells and by a proportion of B CLL cells. Hairy cell leukaemia cells have been demonstrated to strongly express the CD22 antigen. CD22 functions as a sialic acid binding lectin specifically sialic acid in alpha-2,6 linkage. Sialylation of CD22 by alpha-2,6 sialyl transferase inhibits CD22 ligand binding.
      Antigen distribution:
      B cells (lg+, E-) 92 + 3%
      Peripheral blood lymphocytes 12 + 3%
      Thymocytes < 1%
      Granulocytes < 1%
      Monocytes > 1%
      T cells (E+) > 1%
      FUSION PARTNER: NS1 myeloma cell line
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CD22
      • Siglec-2
      • SIGLEC-2
      • BL-CAM
      • FLJ22814
      • MGC130020
      • Leu-14
      • SIGLEC2
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P20273 # Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.
      SIZE: 847 amino acids; 95348 Da
      SUBUNIT: Predominantly monomer of isoform CD22-beta. Also found as heterodimer of isoform CD22-beta and a shorter isoform. Interacts with PTPN6/SHP-1, LYN, SYK, PIK3R1/PIK3R2 and PLCG1 upon phosphorylation. Interacts with GRB2, INPP5D and SHC1 upon phosphorylation (By similarity). May form a complex with INPP5D/SHIP, GRB2 and SHC1.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      TISSUE SPECIFICITY: B-lymphocytes.
      DOMAIN: SwissProt: P20273 Contains 4 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2- containing phosphatases.
      PTM: Phosphorylation of Tyr-762, Tyr-807 and Tyr-822 are involved in binding to SYK, GRB2 and SYK, respectively. Phosphorylation of Tyr-842 is involved in binding to SYK, PLCG2 and PIK3R1/PIK3R2. & Phosphorylated on tyrosine residues by LYN (By similarity).
      SIMILARITY: Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. & Contains 6 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 Ig-like V-type (immunoglobulin-like) domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsFor use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-BL-CAM, clone RFB4 - 2121749 2121749
      Anti-BL-CAM, clone RFB4 - 2360941 2360941
      Anti-BL-CAM, clone RFB4 - NG1858234 NG1858234

      References

      Reference overviewPub Med ID
      CD22 antigen: biosynthesis, glycosylation and surface expression of a B lymphocyte protein involved in B cell activation and adhesion.
      Schwartz-Albiez, R, et al.
      Int. Immunol., 3: 623-33 (1991)  1991

      Show Abstract
      1716973 1716973

      Data Sheet

      Title
      Anti-BL-CAM, clone RFB4 - Data Sheet

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies