05-1576 | Anti-BOB-1 Antibody, clone 6E4

05-1576
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, IHCRPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1576
      DescriptionAnti-BOB-1 Antibody, clone 6E4
      Alternate Names
      • POU class 2 associating factor 1
      • POU domain class 2, associating factor 1
      • OCT-binding factor 1
      • POU domain, class 2, associating factor 1
      • B-cell-specific coactivator OBF-1
      • POU domain class 2-associating factor 1
      Background InformationB-cell-specific coactivator OBF-1(BOB-1) is a transcriptional coactivator protein specific to B-cells. BOB-1 has been shown to interact with transcriptional factors OCT1 and OCT2, binding to their POU domain and mediating transcriptional activity of octamer motifs to varying degrees. Reportedly, BOB-1 is a required component of both the B-cell to antigen response and germinal center formation. The highest observed expression for BOB-1 is in plasma cells and B-cell germinal centers. Expression of BOB-1 has also been observed in B-cell tumors; however, it is considered to be variable.
      References
      Product Information
      FormatPurified
      Control
      • Daudi cell lysate
      PresentationPurified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-BOB-1 Antibody, clone 6E4 is an antibody against BOB-1 for use in WB, IH.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry:
      Using a representative lot of this antibody, an idependent laboratory reported detection of BOB-1 in spleen and lymph node of immunized mice.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to mouse BOB-1.
      Clone6E4
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRat
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • BOB-1
      • BOB1
      • POU
      • OBF1
      • OBF-1
      • OCAB
      • OCA-B
      • POU2AF1
      Purification MethodProtein G
      UniProt Number
      UniProt SummaryFUNCTION: Transcriptional coactivator that specifically associates with either OCT1 or OCT2. Its boost the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centres.

      SUBCELLULAR LOCATION: Nucleus.

      TISSUE SPECIFICTY: B-cell specific.

      PTM: Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation (By similarity).

      SEQUENCE SIMILARITIES: Belongs to the POU2AF1 family.
      Molecular Weight ~ 32 kDa observed. Other sizes may be observed due to ubiquination.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in Daudi cell lysate.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected BOB-1 on 10 µg of Daudi cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications