05-641 | Anti-BRAF35 Antibody, clone 4.21

200 µg  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWBMPurifiedMonoclonal Antibody
      Catalogue Number05-641
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-BRAF35 Antibody, clone 4.21
      Product Information
      Presentation0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
      ApplicationDetect BRAF35 with Anti-BRAF35 Antibody, clone 4.21 (Mouse Monoclonal Antibody), that has been shown to work in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenGST fusion protein corresponding to residues 100-317 of human BRAF35
      Cloneclone 4.21
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HMG20B
      • SMARCE1r
      • pp8857
      • PP7706
      • BRAF35
      • SOXL
      • FLJ26127
      • SMARCE1R
      • BRAF25
      • HMGX2
      Purification MethodProtein G Chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q9P0W2 # Required for correct progression through G2 phase of the cell cycle and entry into mitosis. Required for RCOR1/CoREST mediated repression of neuronal specific gene promoters.
      SIZE: 317 amino acids; 35813 Da
      SUBUNIT: Component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B/BRAF35, AOF2/LSD1, RCOR1/CoREST and PHF21A/BHC80. The BHC complex may also contain ZMYM2, ZNF217, ZMYM3, GSE1 and GTF2I. Interacts with the BRCA2 tumor suppressor protein.
      SUBCELLULAR LOCATION: Nucleus. Note=Localized to condensed chromosomes in mitosis in conjunction with BRCA2.
      TISSUE SPECIFICITY: Ubiquitously expressed in adult tissues.
      SIMILARITY: SwissProt: Q9P0W2 ## Contains 1 HMG box DNA-binding domain.
      Molecular Weight35kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on HeLa , A431, or 3T3 cell lysates
      Sales RestrictionsDue to license agreement restrictions, this product cannot be purchased for resale.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-BRAF35, clone 4.21 - 22148 22148
      Anti-BRAF35, clone 4.21 -2645294 2645294


      Reference overviewPub Med ID
      Pbx acts with Hand2 in early myocardial differentiation.
      Maves L, Tyler A, Moens CB, Tapscott SJ.
      Developmental biology  333  409-18  2001

      Show Abstract Full Text Article
      19607825 19607825
      A human BRCA2 complex containing a structural DNA binding component influences cell cycle progression.
      Marmorstein, L Y, et al.
      Cell, 104: 247-57 (2001)  2001

      Show Abstract
      11207365 11207365


      RNA-Binding Protein Immunoprecipitation