05-605 | Anti-Bad Antibody, clone BYC001

05-605
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MIP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-605
      Replaces04-432
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Bad Antibody, clone BYC001
      References
      Product Information
      FormatPurified
      Presentation0.1M Tris-glycine, pH 7.4, 0.15M NaCl and 0.05% sodium azide.
      Applications
      ApplicationThis Anti-Bad Antibody, clone BYC001 is validated for use in IP, WB for the detection of Bad.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenFull-length murine Bad containing a N-terminal His6-tag
      CloneBYC001
      HostMouse
      SpecificityBad
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.
      Gene Symbol
      • BAD
      • BBC2
      • BBC6
      • BCL2L8
      Purification MethodProtein G Chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q92934 # Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
      SIZE: 168 amino acids; 18392 Da
      SUBUNIT: Forms heterodimers with the anti-apoptotic proteins, Bcl- X(L), Bcl-2 and Bcl-W. Also binds protein S100A10 (By similarity). The Ser-75/Ser-99 phosphorylated form binds 14-3-3 proteins (By similarity).
      SUBCELLULAR LOCATION: Mitochondrion outer membrane. Cytoplasm. Note=Upon phosphorylation, locates to the cytoplasm.
      TISSUE SPECIFICITY: Expressed in a wide variety of tissues.
      DOMAIN:SwissProt: Q92934 Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
      PTM: Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro- apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation (By similarity).
      SIMILARITY: Belongs to the Bcl-2 family.
      Molecular Weight23kDa (human), 31kDa (mouse)
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on recombinant murine Bad or on RIPA lysates from human MDA cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Bad, clone BYC001 (mouse monoclonal IgG) - 2359955 2359955
      Anti-Bad, clone BYC001 (mouse monoclonal IgG) - DAM1731472 DAM1731472
      Anti-Bad, clone BYC001 - 1962114 1962114
      Anti-Bad, clone BYC001 - 20350 20350
      Anti-Bad, clone BYC001 - 24120 24120
      Anti-Bad, clone BYC001 - 30032 30032
      Anti-Bad, clone BYC001 - DAM1658691 DAM1658691

      References

      Reference overviewApplicationPub Med ID
      Modulation of Pro-survival Akt/Protein Kinase B and ERK1/2 Signaling Cascades by Quercetin and Its in Vivo Metabolites Underlie Their Action on Neuronal Viability
      Spencer, J. P., et al
      J Biol Chem, 278:34783-34793 (2003)  2003

      Immunoblotting (Western)12826665 12826665

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies