04-432 | Anti-Bad (NT) Antibody, rabbit monoclonal

04-432
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RIF, IP, WB, IH(P)RbPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-432
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Bad (NT) Antibody, rabbit monoclonal
      References
      Product Information
      FormatPurified
      PresentationIn 60% storage buffer (50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% sodium azide and 0.05% BSA) and 40% glycerol.
      Applications
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-Bad (N-term) Antibody, Rabbit is an antibody against Bad (N-term) for use in IF, IP, WB, IH(P).
      Key Applications
      • Immunofluorescence
      • Immunoprecipitation
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Biological Information
      ImmunogenKLH-conjugated synthetic peptide corresponding to the N-terminal region of human Bad (N-term).
      HostRabbit
      SpecificityRecognizes a region near the N-terminus domain of human Bad (N-term).
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.
      Gene Symbol
      • BAD
      • BBC2
      • BBC6
      • BCL2L8
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q92934 # Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
      SIZE: 168 amino acids; 18392 Da
      SUBUNIT: Forms heterodimers with the anti-apoptotic proteins, Bcl- X(L), Bcl-2 and Bcl-W. Also binds protein S100A10 (By similarity). The Ser-75/Ser-99 phosphorylated form binds 14-3-3 proteins (By similarity).
      SUBCELLULAR LOCATION: Mitochondrion outer membrane. Cytoplasm. Note=Upon phosphorylation, locates to the cytoplasm.
      TISSUE SPECIFICITY: Expressed in a wide variety of tissues.
      DOMAIN:SwissProt: Q92934 Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
      PTM: Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro- apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation (By similarity).
      SIMILARITY: Belongs to the Bcl-2 family.
      Molecular Weight23 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoprecipitating
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 year at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Bad (N-term) (Rabbit monoclonal IgG) - 2361787 2361787
      Anti-Bad (N-term) (Rabbit monoclonal IgG) - 2365938 2365938
      Anti-Bad (N-term) (Rabbit monoclonal IgG) - 2383054 2383054
      Anti-Bad (N-term) (Rabbit monoclonal IgG) - 2433255 2433255
      Anti-Bad (N-term) (Rabbit monoclonal IgG) - NG1944014 NG1944014
      Anti-Bad (N-term) - 1967328 1967328
      Anti-Bad (N-term) - 2051317 2051317
      Anti-Bad (N-term) - DAM1658192 DAM1658192
      Anti-Bad (N-term) - DAM1731531 DAM1731531
      Anti-Bad (N-term) - DAM1734801 DAM1734801

      References

      Reference overviewPub Med ID
      The RNA component of human telomerase
      Feng, J, et al
      Science, 269:1236-41 (1995)  1995

      7544491 7544491
      Identification of a specific telomere terminal transferase activity in Tetrahymena extracts
      Greider, C W and Blackburn, E H
      Cell, 43:405-13 (1985)  1985

      3907856 3907856

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies