04-434 | Anti-Bax Antibody, rabbit monoclonal

04-434
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RIP, WB, IH(P)RbPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-434
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Bax Antibody, rabbit monoclonal
      References
      Product Information
      FormatPurified
      PresentationIn 60% storage buffer (50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% sodium azide and 0.05% BSA) and 40% glycerol.
      Applications
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-Bax Antibody, Rabbit is validated for use in IP, WB, IH(P) for the detection of Bax.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunoblot Analysis:
      A 1:2,000 dilution of the lot detected endogenous Bax in lysate from Hela cells.

      Immunoprecipitation:
      A 1:100 dilution of this antibody immunoprecipitated Bax using protein G:agarose beads.

      Immunohistochemistry:
      1:250 of this antibody was used in Immunohistochemical analysis of Paraffin-embedded human lymph node.
      Biological Information
      ImmunogenSynthetic peptide corresponding to amino acid residues near the dimerisation domain of human Bax.
      HostRabbit
      SpecificityRecognizes a region near the dimerisation domain of human Bax.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene.
      Gene Symbol
      • BAX
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q07814 # Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor Bcl-2 or its adenovirus homolog E1B 19k protein.| Q07812 # Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Induces the release of cytochrome c, activation of CASP3, and thereby apoptosis.
      SIZE: 41 amino acids; 4678 Da
      SUBUNIT: Interacts with HN.
      SUBCELLULAR LOCATION: Cytoplasm.
      DOMAIN: SwissProt: Q07815
      DISEASE:SwissProt: Q07812 # Defects in BAX are found in some cell lines from hematopoietic malignancies as T-cell acute lymphoblastic leukemia, Burkitt lymphoma, and plasmacytoma.
      SIMILARITY: SwissProt: Q07815 ## Belongs to the Bcl-2 family. | Q07814 # Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.# Belongs to the Bcl-2 family. | P55269 ## Belongs to the Bcl-2 family. | Q07812 # Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family (By similarity).# Belongs to the Bcl-2 family.
      Molecular Weight21 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 year at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Bax (Rabbit monoclonal IgG) - 2424617 2424617
      Anti-Bax (Rabbit monoclonal IgG) - 1955323 1955323
      Anti-Bax (Rabbit monoclonal IgG) - 2283780 2283780
      Anti-Bax (Rabbit monoclonal IgG) - DAM1437294 DAM1437294
      Anti-Bax (Rabbit monoclonal IgG) -2492230 2492230
      Anti-Bax (Rabbit monoclonal IgG) -2494175 2494175
      Anti-Bax - 2039768 2039768
      Anti-Bax - DAM1588276 DAM1588276
      Anti-Bax - DAM1636118 DAM1636118
      Anti-Bax - DAM1658711 DAM1658711

      References

      Reference overviewApplicationPub Med ID
      Folate deficiency decreases apoptosis of endometrium decidual cells in pregnant mice via the mitochondrial pathway.
      Liao, XG; Li, YL; Gao, RF; Geng, YQ; Chen, XM; Liu, XQ; Ding, YB; Mu, XY; Wang, YX; He, JL
      Nutrients  7  1916-32  2015

      Show Abstract
      25781218 25781218
      Apigenin Induces Apoptosis through Mitochondrial Dysfunction in U-2 OS Human Osteosarcoma Cells and Inhibits Osteosarcoma Xenograft Tumor Growth in Vivo.
      Chin-Chung Lin,Ya-Ju Chuang,Chien-Chih Yu,Jai-Sing Yang,Chi-Cheng Lu,Jo-Hua Chiang,Jing-Pin Lin,Nou-Ying Tang,An-Cheng Huang,Jing-Gung Chung
      Journal of agricultural and food chemistry  60  2012

      Show Abstract
      23066961 23066961
      Triggering apoptotic death of human malignant melanoma a375.s2 cells by bufalin: involvement of caspase cascade-dependent and independent mitochondrial signaling pathways.
      Hsiao, YP; Yu, CS; Yu, CC; Yang, JS; Chiang, JH; Lu, CC; Huang, HY; Tang, NY; Yang, JH; Huang, AC; Chung, JG
      Evidence-based complementary and alternative medicine : eCAM  2012  591241  2012

      Show Abstract
      22719785 22719785
      Phenethyl Isothiocyanate (PEITC) Inhibits the Growth of Human Oral Squamous Carcinoma HSC-3 Cells through G(0)/G(1) Phase Arrest and Mitochondria-Mediated Apoptotic Cell Death.
      Chen, PY; Lin, KC; Lin, JP; Tang, NY; Yang, JS; Lu, KW; Chung, JG
      Evidence-based complementary and alternative medicine : eCAM  2012  718320  2012

      Show Abstract
      Western Blotting22919418 22919418
      Hierarchical involvement of Bak, VDAC1 and Bax in cisplatin-induced cell death.
      Tajeddine, N; Galluzzi, L; Kepp, O; Hangen, E; Morselli, E; Senovilla, L; Araujo, N; Pinna, G; Larochette, N; Zamzami, N; Modjtahedi, N; Harel-Bellan, A; Kroemer, G
      Oncogene  27  4221-32  2008

      Show Abstract
      Western Blotting18362892 18362892
      Bax interacts with the permeability transition pore to induce permeability transition and cytochrome c release in isolated mitochondria
      Narita, M, et al
      Proc Natl Acad Sci USA, 95:14681-6 (1998)  1998

      9843949 9843949

      Brochure

      Title
      Comprehensive solutions for studying cell health - Life, death, and everything in between.

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies