05-826 | Anti-Bcl-2 Antibody, clone AW604

200 µg  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIP, WBMPurifiedMonoclonal Antibody
      Catalogue Number05-826
      Replaces04-436; OP60-100UG
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Bcl-2 Antibody, clone AW604
      Product Information
      Presentation0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
      ApplicationThis Anti-Bcl-2 Antibody, clone AW604 is validated for use in IP, WB for the detection of Bcl-2.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide corresponding to amino acids 41-54 (C-GAAPAPGIFSSQPG) of human Bcl-2
      Cloneclone AW604
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.
      Gene Symbol
      • BCL2
      • Bcl-2
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P10415 # Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).
      SIZE: 239 amino acids; 26266 Da
      SUBUNIT: Forms homodimers, and heterodimers with BAX, BAD, BAK and Bcl-X(L). Heterodimerization with BAX requires intact BH1 and BH2 motifs, and is necessary for anti-apoptotic activity (By similarity). Also interacts with APAF1, RAF-1, TP53BP2, BBC3, BCL2L1, MRPL41 and BNIPL. Binding to FKBP8 seems to target BCL2 to the mitochondria and probably interferes with the binding of BCL2 to its targets.
      SUBCELLULAR LOCATION: Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein.
      TISSUE SPECIFICITY: Expressed in a variety of tissues.
      DOMAIN: SwissProt: P10415 The BH4 motif is required for anti-apoptotic activity and for interaction with RAF-1.
      PTM: Phosphorylation/dephosphorylation on Ser-70 regulates anti- apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Dephosphorylated by protein phosphatase 2A (PP2A) (By similarity). & Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.
      DISEASE: SwissProt: P10415 # A chromosomal aberration involving BCL2 may be a cause of follicular lymphoma (FL) [MIM:151430]; also known as type II chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
      SIMILARITY: Belongs to the Bcl-2 family.
      Molecular Weight26kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on Raji RIPA cell lysate
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Bcl2, clone AW604 (mouse monoclonal IgG2a) 2905310
      Anti-Bcl2, clone AW604 (mouse monoclonal IgG2a) 2949939
      Anti-Bcl2, clone AW604 (mouse monoclonal IgG2a) - 2250531 2250531
      Anti-Bcl2, clone AW604 (mouse monoclonal IgG2a) - 2359956 2359956
      Anti-Bcl2, clone AW604 (mouse monoclonal IgG2a) - DAM1606750 DAM1606750
      Anti-Bcl2, clone AW604 (mouse monoclonal IgG2a) - DAM1644548 DAM1644548
      Anti-Bcl2, clone AW604 - 0703054135 0703054135
      Anti-Bcl2, clone AW604 - 2054859 2054859
      Anti-Bcl2, clone AW604 - 2515019 2515019
      Anti-Bcl2, clone AW604 - 26997 26997


      Reference overviewPub Med ID
      Prevention of apoptosis by Bcl-2: release of cytochrome c from mitochondria blocked.
      Yang, J, et al.
      Science, 275: 1129-32 (1997)  1997

      Show Abstract
      9027314 9027314
      Investigation of the subcellular distribution of the bcl-2 oncoprotein: residence in the nuclear envelope, endoplasmic reticulum, and outer mitochondrial membranes
      Krajewski, S, et al
      Cancer Res, 53:4701-14 (1993)  1993

      8402648 8402648
      Differential expression of bcl2 protooncogene in neuroblastoma and other human tumor cell lines of neural origin
      Reed, J C, et al
      Cancer Res, 51:6529-38 (1991)  1991

      1742726 1742726
      Programmed cell death: apoptosis and oncogenesis.
      Williams, G T
      Cell, 65: 1097-8 (1991)  1991

      1648446 1648446
      Expression of the bcl-2 oncogene protein is not specific for the 14;18 chromosomal translocation.
      Pezzella, F, et al.
      Am. J. Pathol., 137: 225-32 (1990)  1990

      Show Abstract
      2201196 2201196

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      Life Science Research > Antibodies and Assays > Primary Antibodies