04-436 | Anti-Bcl-2 Antibody, rabbit monoclonal

04-436
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MFC, IF, IP, WB, IH(P)RbPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-436
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Bcl-2 Antibody, rabbit monoclonal
      References
      Product Information
      FormatPurified
      PresentationIn 60% storage buffer (50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% sodium azide and 0.05% BSA) and 40% glycerol.
      Applications
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect Bcl-2 using this Anti-Bcl-2 Antibody, Rabbit validated for use in FC, IF, IP, WB, IH(P).
      Key Applications
      • Flow Cytometry
      • Immunofluorescence
      • Immunoprecipitation
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Biological Information
      ImmunogenKLH-conjugated synthetic peptide between residues BH3 and BH4 of human Bcl-2.
      HostRabbit
      SpecificityRecognizes a region between residues BH3 and BH4 of human Bcl-2.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.
      Gene Symbol
      • BCL2
      • Bcl-2
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P10415 # Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).
      SIZE: 239 amino acids; 26266 Da
      SUBUNIT: Forms homodimers, and heterodimers with BAX, BAD, BAK and Bcl-X(L). Heterodimerization with BAX requires intact BH1 and BH2 motifs, and is necessary for anti-apoptotic activity (By similarity). Also interacts with APAF1, RAF-1, TP53BP2, BBC3, BCL2L1, MRPL41 and BNIPL. Binding to FKBP8 seems to target BCL2 to the mitochondria and probably interferes with the binding of BCL2 to its targets.
      SUBCELLULAR LOCATION: Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein.
      TISSUE SPECIFICITY: Expressed in a variety of tissues.
      DOMAIN: SwissProt: P10415 The BH4 motif is required for anti-apoptotic activity and for interaction with RAF-1.
      PTM: Phosphorylation/dephosphorylation on Ser-70 regulates anti- apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Dephosphorylated by protein phosphatase 2A (PP2A) (By similarity). & Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.
      DISEASE: SwissProt: P10415 # A chromosomal aberration involving BCL2 may be a cause of follicular lymphoma (FL) [MIM:151430]; also known as type II chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
      SIMILARITY: Belongs to the Bcl-2 family.
      Molecular Weight26 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 year at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Bcl-2 (Rabbit monoclonal IgG) - DAM1421469 DAM1421469
      Anti-Bcl-2 - DAM1548633 DAM1548633
      Anti-Bcl-2 - DAM1597402 DAM1597402
      Anti-Bcl-2 - DAM1612208 DAM1612208
      Anti-Bcl-2 - NG1795395 NG1795395
      Anti-Bcl-2, Rabbit Monoclonal 2464525
      Anti-Bcl-2, Rabbit Monoclonal - 2387726 2387726
      Anti-Bcl-2, Rabbit Monoclonal - 2420334 2420334
      Anti-Bcl-2, Rabbit Monoclonal - 2424622 2424622
      Anti-Bcl-2, Rabbit Monoclonal - 0701049312 0701049312

      References

      Reference overviewPub Med ID
      Oral inoculation of probiotics Lactobacillus acidophilus NCFM suppresses tumour growth both in segmental orthotopic colon cancer and extra-intestinal tissue.
      Chen, CC; Lin, WC; Kong, MS; Shi, HN; Walker, WA; Lin, CY; Huang, CT; Lin, YC; Jung, SM; Lin, TY
      The British journal of nutrition  107  1623-34  2012

      Show Abstract
      21992995 21992995
      Autophagy is involved in traumatic brain injury-induced cell death and contributes to functional outcome deficits in mice.
      C-L Luo,B-X Li,Q-Q Li,X-P Chen,Y-X Sun,H-J Bao,D-K Dai,Y-W Shen,H-F Xu,H Ni,L Wan,Z-H Qin,L-Y Tao,Z-Q Zhao
      Neuroscience  184  2011

      Show Abstract
      21463664 21463664
      Phosphorylation of Bcl2 and regulation of apoptosis
      Ruvolo, P P, et al
      Leukemia, 15:515-22 (2001)  2001

      11368354 11368354
      Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death
      Hockenbery, D, et al
      Nature, 348:334-6 (1990)  1990

      2250705 2250705

      Brochure

      Title
      Comprehensive solutions for studying cell health - Life, death, and everything in between.

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies