04-958 | Anti-Beta Catenin Antibody, clone 7F7.2

04-958
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-958
      Replaces04-1002
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Beta Catenin Antibody, clone 7F7.2
      Alternate Names
      • Beta Catenin
      • Catenin (cadherin-associated protein), beta 1
      Background InformationCatenins are proteins found in complexes with cadherin cell adhesion molecules of animal cells. The first two catenins that were identified became known as α-catenin and β-catenin. α-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. When β-catenin was sequenced it was found to be a member of the armadillo family of proteins. These proteins have multiple copies of the so-called armadillo repeat domain which is specialized for protein-protein binding. An increase in β-catenin production has been noted in those people who have Basal Cell Carcinoma and leads to the increase in proliferation of related tumors. When β-catenin is not associated with cadherins and α-catenin, it can interact with other proteins such as Catenin Beta Interacting Protein 1 (ICAT) and adenomatosis polyposis coli (APC). Recent evidence suggests that β-catenin plays an important role in various aspects of liver biology including liver development (both embryonic and postnatal), liver regeneration following partial hepatectomy, HGF-induced hepatpomegaly, liver zonation, and pathogenesis of liver cancer.
      References
      Product Information
      FormatPurified
      Control
      • HEK293 cell lysate, PC12 cell lysate, NIH/3T3 cell lysate
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationThis Anti-Beta Catenin Antibody, clone 7F7.2 is validated for use in WB, IC for the detection of Beta Catenin.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesWestern Blot Analysis: 0.5-2 µg/mL of this lot detected beta catenin in HEK293 cell lysate.
      Biological Information
      ImmunogenFull length, GST-tagged recombinant beta catenin.
      Clone7F7.2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes beta catenin.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryBeta-catenin is an adherens junction protein. Adherens junctions (AJs; also called the zonula adherens) are critical for the establishment and maintenance of epithelial layers, such as those lining organ surfaces. AJs mediate adhesion between cells, communicate a signal that neighboring cells are present, and anchor the actin cytoskeleton. In serving these roles, AJs regulate normal cell growth and behavior. At several stages of embryogenesis, wound healing, and tumor cell metastasis, cells form and leave epithelia. This process, which involves the disruption and reestablishment of epithelial cell-cell contacts, may be regulated by the disassembly and assembly of AJs. AJs may also function in the transmission of the 'contact inhibition' signal, which instructs cells to stop dividing once an epithelial sheet is complete.[supplied by OMIM]
      Gene Symbol
      • CTNNB
      • CTNNB1
      • FLJ25606
      • FLJ37923
      • DKFZp686D02253
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Involved in the regulation of cell adhesion and in signal transduction through the Wnt pathway.
      SUBUNIT: Two separate pools are found in the cytoplasm: one is PSEN1/cadherin/catenin complex which anchors to the actin cytoskeleton. The other pool is part of a large complex containing AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. Wnt-dependent activation of DVL antagonizes the action of GSK3B. When GSK3B activity is inhibited the complex dissociates, CTNNB1 is dephosphorylated and is no longer targeted for destruction. The stabilized protein translocates to the nucleus, where it binds TCF/LEF-1 family members, TBP, BCL9 and possibly also RUVBL1 and DUPLIN. Binds CTNNBIP and EP300. CTNNB1 forms a ternary complex with LEF1 and EP300 that is disrupted by CTNNBIP1 binding (By similarity). Interacts with the PDZ domain of TAX1BP3; this interaction inhibits the transcriptional activity of CTNNB1 (By similarity). Interacts with AJAP1, BAIAP1, CARM1, CTNNA3, CXADR and PCDH11Y. Binds SLC9A3R1. Interacts with GLIS2 and MUC1. Interacts with SLC30A9. Interacts with XIRP1 (By similarity).
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Cytoplasm, cytoskeleton. Note=Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 and MUC1 promotes nuclear translocation.
      TISSUE SPECIFICITY: Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheats. Expressed in colon.
      Molecular Weight92 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot on HEK293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Beta Catenin, clone 7F7.2 - 2388923 2388923
      Anti-Beta Catenin, clone 7F7.2 - 1952471 1952471
      Anti-Beta Catenin, clone 7F7.2 - 2063196 2063196
      Anti-Beta Catenin, clone 7F7.2 - 2278566 2278566
      Anti-Beta Catenin, clone 7F7.2 - 2510196 2510196
      Anti-Beta Catenin, clone 7F7.2 - 2529960 2529960
      Anti-Beta Catenin, clone 7F7.2 - NG1545025 NG1545025
      Anti-Beta Catenin, clone 7F7.2 - NG1696475 NG1696475
      Anti-Beta Catenin, clone 7F7.2 - NG1726936 NG1726936
      Anti-Beta Catenin, clone 7F7.2 - NG1895106 NG1895106

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies