05-637 | Anti-Bmi-1 Antibody, clone F6

05-637
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, RbICC, IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-637
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Bmi-1 Antibody, clone F6
      Alternate Names
      • Polycomb group RING finger protein 4
      • RING finger protein 51
      Background InformationThe Bmi1 protein is a member of the polycomb group (PcG) proteins. PcG proteins form multiprotein complexes that function as transcriptional repressors.
      Several lines of evidence implicate Bmi1 in tumorigenesis. Firstly, Bmi1 cooperates with c-Myc in the generation of lymphomas in double transgenic mice. Secondly, Bmi1 blocks senescence and immortalizes mouse embryo fibroblasts, although not human fibroblasts, and in combination with an activated H-ras gene leads to neoplastic transformation. Thirdly, the BMI1 gene is amplified in certain mantle cell lymphomas and is overexpressed in a subset of non-small cell lung cancer, colorectal carcinomas, multiple myelomas and medulloblastomas, but not glioblastomas. Fourthly, the transformation of human hematopoietic progenitor cells by the oncogenic fusion protein E2a-Pbx1 requires Bmi1. Indeed, based on a list of genes differentially expressed in a mouse model of metastatic prostate cancer on a wild-type and bmi1-deficient genetic background a gene signature was recently identified that may predict response to therapy and survival in multiple types of cancer.
      References
      Product Information
      FormatPurified
      Control
      • U2OS whole cell lysate, K-562 nuclear extract, 0-4 hour embryos or 12-24 hour embryos
      PresentationImmunoaffinity Purified immunoglobulin in Protein A Purified immunoglobulin in 0.1M Tris-Glycine, 0.15M NaCl, 0.05% sodium azide, pH 7.4.
      Applications
      ApplicationAnti-Bmi-1 Antibody, clone F6 is a Mouse Monoclonal Antibody for detection of Bmi-1 also known as Polycomb group RING finger protein 4, RING finger protein 51 and has been validated in ICC, IP & WB.
      Key Applications
      • Immunocytochemistry
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenImmunized with recombinant Bmi-1 protein corresponding to residues 1-202 of mouse Bmi-1.
      CloneF6
      HostMouse
      SpecificityRecognizes Bmi-1 (triplet).
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Rabbit
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • BMI1
      • MGC12685
      • PCGF4
      • flvi-2/bmi-1
      • RNF51
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P35226 # Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
      SIZE: 326 amino acids; 36949 Da
      SUBUNIT: Component of chromatin-associated class II polycomb repressive complex 1 (PRC1/hPRC-H) at least composed of PCGF2/RNF110, BMI1/PCGF4, CBX2/M33, CBX4/PC2, CBX8/PC3, PHC1, PHC2, PHC3, SCMH1, RING1 and RNF2/RING2. Also component of E3 ubiquitin ligase complex or human polycomb repressive complex 1- like at least composed of PHC2, BMI1, RING1 and RNF2. Interacts with SPOP. Part of a complex consisting of BMI1, CUL3 and SPOP.
      SUBCELLULAR LOCATION: Nucleus.
      PTM: May be polyubiquitinated; which does not lead to proteasomal degradation.DISEASE:SwissProt: P35226 # Cooperates with the MYC oncogene to produce B-lymphomas.
      SIMILARITY: SwissProt: P35226 ## Contains 1 RING-type zinc finger.
      Molecular Weight40-44 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from U2OS cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Bmi-1, clone F6 2462874
      Anti-Bmi-1, clone F6 (mouse monoclonal IgG1) Monoclonal Antibody Q2922299
      Anti-Bmi-1, clone F6 - 2153145 2153145
      Anti-Bmi-1, clone F6 - 2382167 2382167
      Anti-Bmi-1, clone F6 - 2430420 2430420
      Anti-Bmi-1, clone F6 - 1624901 1624901
      Anti-Bmi-1, clone F6 - 1957274 1957274
      Anti-Bmi-1, clone F6 - 1975350 1975350
      Anti-Bmi-1, clone F6 - 2019726 2019726
      Anti-Bmi-1, clone F6 - 22103 22103

      References

      Reference overviewApplicationSpeciesPub Med ID
      Retrovirus-Mediated Expression of E2A-PBX1 Blocks Lymphoid Fate but Permits Retention of Myeloid Potential in Early Hematopoietic Progenitors.
      Woodcroft, MW; Nanan, K; Thompson, P; Tyryshkin, K; Smith, SP; Slany, RK; LeBrun, DP
      PloS one  10  e0130495  2015

      Show Abstract
      26098938 26098938
      Psip1/Ledgf p75 restrains Hox gene expression by recruiting both trithorax and polycomb group proteins.
      Pradeepa, MM; Grimes, GR; Taylor, GC; Sutherland, HG; Bickmore, WA
      Nucleic acids research  42  9021-32  2014

      Show Abstract
      25056311 25056311
      Silencing BMI1 eliminates tumor formation of pediatric glioma CD133+ cells not by affecting known targets but by down-regulating a novel set of core genes.
      Baxter, PA; Lin, Q; Mao, H; Kogiso, M; Zhao, X; Liu, Z; Huang, Y; Voicu, H; Gurusiddappa, S; Su, JM; Adesina, AM; Perlaky, L; Dauser, RC; Leung, HC; Muraszko, KM; Heth, JA; Fan, X; Lau, CC; Man, TK; Chintagumpala, M; Li, XN
      Acta neuropathologica communications  2  160  2014

      Show Abstract
      Western Blotting25526772 25526772
      Transcription factors FOXG1 and Groucho/TLE promote glioblastoma growth.
      Verginelli, F; Perin, A; Dali, R; Fung, KH; Lo, R; Longatti, P; Guiot, MC; Del Maestro, RF; Rossi, S; di Porzio, U; Stechishin, O; Weiss, S; Stifani, S
      Nature communications  4  2956  2013

      Show Abstract
      24356439 24356439
      Alu elements in ANRIL non-coding RNA at chromosome 9p21 modulate atherogenic cell functions through trans-regulation of gene networks.
      Holdt, LM; Hoffmann, S; Sass, K; Langenberger, D; Scholz, M; Krohn, K; Finstermeier, K; Stahringer, A; Wilfert, W; Beutner, F; Gielen, S; Schuler, G; Gäbel, G; Bergert, H; Bechmann, I; Stadler, PF; Thiery, J; Teupser, D
      PLoS genetics  9  e1003588  2013

      Show Abstract
      23861667 23861667
      Ring1b bookmarks genes in pancreatic embryonic progenitors for repression in adult β cells.
      van Arensbergen, J; García-Hurtado, J; Maestro, MA; Correa-Tapia, M; Rutter, GA; Vidal, M; Ferrer, J
      Genes & development  27  52-63  2013

      Show Abstract
      23271347 23271347
      Combined modulation of polycomb and trithorax genes rejuvenates β cell replication.
      Zhou, JX; Dhawan, S; Fu, H; Snyder, E; Bottino, R; Kundu, S; Kim, SK; Bhushan, A
      The Journal of clinical investigation  123  4849-58  2013

      Show Abstract
      24216481 24216481
      Inflammation increases cells expressing ZSCAN4 and progenitor cell markers in the adult pancreas.
      Ko, SB; Azuma, S; Yokoyama, Y; Yamamoto, A; Kyokane, K; Niida, S; Ishiguro, H; Ko, MS
      American journal of physiology. Gastrointestinal and liver physiology  304  G1103-16  2013

      Show Abstract
      Immunofluorescence, Immunohistochemistry23599043 23599043
      Knockdown BMI1 expression inhibits proliferation and invasion in human bladder cancer T24 cells.
      Liang, W; Zhu, D; Cui, X; Su, J; Liu, H; Han, J; Zhao, F; Xie, W
      Molecular and cellular biochemistry  382  283-91  2013

      Show Abstract
      Immunohistochemistry23820733 23820733
      Multipotent stem cells from trabecular meshwork become phagocytic TM cells.
      Du, Y; Roh, DS; Mann, MM; Funderburgh, ML; Funderburgh, JL; Schuman, JS
      Investigative ophthalmology & visual science  53  1566-75  2012

      Show Abstract
      Western Blotting, Fluorescence Activated Cell Sorting (FACS)Human22297497 22297497

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