05-637-AF647 | Anti-Bmi-1 Antibody, clone F6, Alexa Fluor® 647

05-637-AF647
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MkICCMAlexaFluor®647Monoclonal Antibody
      Description
      Catalogue Number05-637-AF647
      DescriptionAnti-Bmi-1 Antibody, clone F6, Alexa Fluor® 647
      Alternate Names
      • Polycomb complex protein BMI-1
      • Polycomb group RING finger protein 4
      • Bmi-1
      Background InformationPolycomb complex protein BMI-1 (UniProt P25916; also known as B lymphoma Mo-MLV insertion region 1, Polycomb group RING finger protein 4) is encoded by the Bmi1 (also known as AW546694, Bmi-1, Pcgf4) gene (Gene ID 12151) in murine species. Bmi1 is a member of the polycomb group (PcG) family of evolutionarily conserved gene silencers involved in cell fate decisions and body pattern during development. Bmi1 is an important regulator of self-renewal of hematopoietic and neural stem cells, as well as cancer stem cells (CSCs). Bmi1 is a main constituent of the polycomb repressive complex 1 (PRC1) and pariticipates in PRC-mediated gene silencing by promoting monoubiquitination of H2A. Bmi1 overexpression is reported in many different human cancers and high level of Bmi1 is considered as a poor prognostic marker. Bmi1 targets genes involved in transforming growth factor-β/bone morphogenetic protein (TGF-beta/BMP), endoplasmic reticulum (ER) stress, and WNT pathways. Bmi itself is transcriptionally regulated by c-Myc and E2F1 transcription factors.
      References
      Product Information
      FormatAlexaFluor®647
      PresentationPurified mouse conjugate antibody in PBS with 15 mg/ml BSA and 0.1% sodium azide.
      Applications
      ApplicationThis Anti-Bmi-1 Antibody, clone F6, Alexa Fluor® 647 is validated for use in Immunocytochemistry for the detection of Bmi-1.
      Key Applications
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Bmi-1 in HepG2 cells.
      The unconjugated version (Cat. No. 05-637) is also shown to be suitable for Western blotting, Immunocytochemistry, and Immunohistochemistry applications.
      Biological Information
      ImmunogenRecombinant Bmi-1 protein corresponding to residues 1-202 of mouse Bmi-1.
      Epitopea.a. 1-202
      CloneF6
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG1
      Species Reactivity
      • Human
      • Monkey
      Species Reactivity NoteHuman, Monkey. Predicted to react with Mouse, Rat, Rabbit based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Bmi1
      • Bmi-1
      • Pcgf4
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight40-44 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Bmi-1 in HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies