05-644 | Anti-CAR Antibody, clone RmcB

05-644
200 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RICC, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-644
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-CAR Antibody, clone RmcB
      Alternate Names
      • 46 kD coxsackievirus and adenovirus receptor (CAR) protein
      • CVB3 binding protein
      • CVB3-binding protein
      • Coxsackievirus B-adenovirus receptor
      • coxsackie virus B receptor
      • coxsackie virus and adenovirus receptor
      Background InformationThe coxsackievirus and adenovirus receptor (CAR) mediates cell attachment and infection by coxsackie B viruses and by a number of adenoviruses. CAR also mediates homotypic intercellular interactions. In polarized epithelial cells, CAR is closely associated with the tight junction, where it contributes to the barrier to paracellular flow of solutes and macromolecules. CAR's biological roles are not well defined, but emerging evidence suggests that it may function during embryonic development and in regulating cell proliferation.
      References
      Product Information
      FormatPurified
      Control
      • Lung tissue
      PresentationPurified mouse monoclonal IgG1 in buffer containing 0.1M Tris-Glycine, 0.15M NaCl and 0.05% Sodium Azide, pH 7.4.
      Applications
      ApplicationAnti-CAR Antibody, clone RmcB detects level of CAR & has been published & validated for use in IC & WB.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      Application NotesImmunocytochemistry:
      1-2 μg/mL of a previous lot showed positive immunostaining for CAR in HeLa cells, hCAR transfected CHO cells, but not in untransfected CHO cells fixed with 4% paraformaldehyde and permeabilized with 0.2% Triton-X.
      Biological Information
      ImmunogenNative human coxsackie virus-adenovirus receptor protein.
      CloneRmcB
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes CAR at MW ~46 kDa.
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a type I membrane receptor for group B coxsackieviruses and subgroup C adenoviruses. Pseudogenes of this gene are found on chromosomes 15, 18, and 21.
      Gene Symbol
      • CXADR
      • CAR
      • hCAR
      • HCVADR
      • HCAR
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P78310 # Receptor for group B coxsackieviruses and subgroup C of adenoviruses (AD2 and AD5), susceptibility to infection is correlated to its level of expression and availability at the plasma membrane of cells.
      SIZE: 365 amino acids; 40030 Da
      SUBUNIT: Monomer. Probably homodimer formed by 2 molecules on adjacent cells. Interacts with LNX, MPDZ, BAIAP1, DLG4, PRKCABP, TJP1 and CTNNB1. Secreted isoform 3, isoform 4 and isoform 5 can interact with the extracellular domain of the receptor. Binds adenovirus 12 fiber protein. Isoform 5 but not isoform 3 binds to coxsackievirus.
      SUBCELLULAR LOCATION: Isoform 1: Cell membrane; Single-pass type I membrane protein. Note=In epithelial cells localizes to the apical junction complex composed of tight and adherens junctions. In airway epithelial cells localized to basolateral membrane but not to apical surface. & Isoform 2: Cell membrane; Single-pass type I membrane protein. Note=In epithelial cells localizes to the apical junction complex composed of tight and adherens junctions. In airway epithelial cells localized to basolateral membrane but not to apical surface. & Isoform 3: Secreted. & Isoform 4: Secreted. & Isoform 5: Secreted.
      TISSUE SPECIFICITY: Expressed in pancreas, brain, heart, small intestine, testis, prostate and at a lower level in liver and lung. Isoform 5 is ubiquitoulsy expressed while isoform 3 is expressed in heart, lung and pancreas. In skeletal muscle, isoform 1 is found at the neuromuscular junction and isoform 2 is found in blood vessels. In cardiac muscle, isoform 1 and isoform 2 are found at intercalated disks. In heart expressed in subendothelial layers of the vessel wall but not in the luminal endothelial surface. Expression is elevated in hearts with dilated cardiomyopathy.
      DOMAIN: SwissProt: P78310 The Ig-like C2-type 1 domain probably mediates homodimerization and interaction with JAML. & The PDZ-binding motif mediates interaction with MPDZ and BAIAP1.
      PTM: N-glycosylated (By similarity). & Palmitoylated on Cys-259 and/or Cys-260; required for proper localization to the plasma membrane.
      SIMILARITY: Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
      Molecular Weight~46 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot on mouse small intestine lysates.

      Western Blot Analysis:
      1:500 dilution of this antibody detected Car on 10 µg of mouse small intestine lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-CAR, clone RmcB 2470905
      Anti-CAR, clone RmcB - 2123703 2123703
      Anti-CAR, clone RmcB - 2379355 2379355
      Anti-CAR, clone RmcB - 2430421 2430421
      Anti-CAR, clone RmcB - 2166061 2166061
      Anti-CAR, clone RmcB - 22154 22154
      Anti-CAR, clone RmcB - 2273968 2273968
      Anti-CAR, clone RmcB - 29859 29859
      Anti-CAR, clone RmcB - JBC1858525 JBC1858525
      Anti-CAR, clone RmcB - JBC1870056 JBC1870056

      References

      Reference overviewApplicationPub Med ID
      Antitumor effects of bladder cancer-specific adenovirus carrying E1A-androgen receptor in bladder cancer.
      Zhai, Z; Wang, Z; Fu, S; Lu, J; Wang, F; Li, R; Zhang, H; Li, S; Hou, Z; Wang, H; Rodriguez, R
      Gene therapy  19  1065-74  2012

      Show Abstract
      22218302 22218302
      Transduction of brain dopamine neurons by adenoviral vectors is modulated by CAR expression: rationale for tropism modified vectors in PD gene therapy.
      Lewis, TB; Glasgow, JN; Glandon, AM; Curiel, DT; Standaert, DG
      PloS one  5  2010

      Show Abstract Full Text Article
      20862245 20862245
      Adenovirus-induced thrombocytopenia: the role of von Willebrand factor and P-selectin in mediating accelerated platelet clearance.
      Othman, M; Labelle, A; Mazzetti, I; Elbatarny, HS; Lillicrap, D
      Blood  109  2832-9  2007

      Show Abstract
      17148587 17148587
      The histone deacetylase inhibitors depsipeptide and MS-275, enhance TRAIL gene therapy of LNCaP prostate cancer cells without adverse effects in normal prostate epithelial cells.
      Kasman, L; Lu, P; Voelkel-Johnson, C
      Cancer gene therapy  14  327-34  2007

      Show Abstract
      Fluorescence Activated Cell Sorting (FACS)17186014 17186014
      Peroxisome proliferator-activated receptor subtype- and cell-type-specific activation of genomic target genes upon adenoviral transgene delivery.
      Nielsen, R; Grøntved, L; Stunnenberg, HG; Mandrup, S
      Molecular and cellular biology  26  5698-714  2006

      Show Abstract
      Fluorescence Activated Cell Sorting (FACS)16847324 16847324
      HCAR and MCAR: the human and mouse cellular receptors for subgroup C adenoviruses and group B coxsackieviruses.
      Tomko, R P, et al.
      Proc. Natl. Acad. Sci. U.S.A., 94: 3352-6 (1997)  1997

      Show Abstract
      9096397 9096397
      A monoclonal antibody specific for the cellular receptor for the group B coxsackieviruses.
      Hsu KH, Lonberg-Holm K, Alstein B, Crowell RL.
      J. Virol., 62:1647-1652 (1988)  1988

      2451756 2451756

      Related Products & Applications

      Related Products

      Catalogue Number Description  
      FCMAB418PE Milli-Mark™ Anti-CAR-PE Antibody, clone RmcB Show Pricing & Availability
      FCMAB418PE-I Milli-Mark™ Anti-CAR-PE Antibody, clone RmcB Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies