CBL150F | Anti-CD3 Antibody, clone UCHT1, FITC conjugated

CBL150F
100 assays  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFC, IHCMFITCMonoclonal Antibody
      Description
      Catalogue NumberCBL150F
      ReplacesCBL126F
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-CD3 Antibody, clone UCHT1, FITC conjugated
      Alternate Names
      • TCR
      References
      Product Information
      FormatFITC
      PresentationThe conjugate is supplied as a 100 test/1 mL vial in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. For flow cytometry we recommend using 10μL of conjugate per test.
      Applications
      ApplicationAnti-CD3 Antibody, clone UCHT1, FITC conjugated is an antibody against CD3 for use in FC, IH.
      Key Applications
      • Flow Cytometry
      • Immunohistochemistry
      Application NotesFlow Cytometry

      Identification of CD3 positive T lymphocytes by indirect immunofluorescence staining

      Identification of normal and malignant T lymphocytes in frozen tissue sections

      Immunoprecipitation of the CD3 antigen

      Mitogenic stimulation of T lymphocytes with or without the presence of IL-2

      Leukaemia and lymphoma phenotyping

      Optimal dilutions must be determined by end user.
      Biological Information
      ImmunogenPurified human T cells
      CloneUCHT1
      HostMouse
      SpecificityThis antibody recognizes 22/26/30 kDa molecular weight lymphocyte surface molecules associated with the T cell antigen receptor complex. It reacts with the majority of peripheral blood T lymphocytes, a major proportion of thymocytes, the majority of T cell chronic lymphocytic leukaemia cells, Sézary leukaemias and approximately 70% of acute lymphoblastic leukaemias of T cell origin.
      Antigen distribution:
      T cells 100%
      Peripheral Blood lymphocytes 75 + 7%
      Thymocytes 68 + 5%
      Granulocytes < 1%
      Monocytes < 1%
      B cells (E-) < 1%
      NK cells (CD16+) < 1%
      KNOWN SPECIES CROSS REACTIVITY: Negative with rhesus monkey peripheral blood leucocytes in flow cytometry
      FUSION PARTNER: P3 x 63 Ag8.653 myeloma cell line
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women.
      Gene Symbol
      • CD3E
      • T3E
      • TCRE
      Non-Reactive Species
      • Monkey
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P07766 # The CD3 complex mediates signal transduction.
      SIZE: 207 amino acids; 23147 Da
      SUBUNIT: The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      SIMILARITY: SwissProt: P07766 ## Contains 1 Ig-like (immunoglobulin-like) domain. & Contains 1 ITAM domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at +4°C protected from light. DO NOT FREEZE. For long term use and storage aliquot conjugate into small volumes and store at +4°C.
      Packaging Information
      Material Size100 assays
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-CD3, clone UCHT1, FITC conjugated - CBL150F CBL150F
      Anti-CD3, clone UCHT1, FITC conjugated - 2367811 2367811

      References

      Reference overviewPub Med ID
      Leukocyte cell surface enzymology: CD45 (LCA, T200) is a protein tyrosine phosphatase.
      Clark, E A and Ledbetter, J A
      Immunol. Today, 10: 225-8 (1989)  1989

      Show Abstract
      2553046 2553046
      The transmembrane orientation of the epsilon chain of the TcR/CD3 complex.
      Clevers, H, et al.
      Eur. J. Immunol., 18: 705-10 (1988)  1988

      Show Abstract
      2967760 2967760
      The T cell receptor/CD3 complex: a dynamic protein ensemble.
      Clevers, H, et al.
      Annu. Rev. Immunol., 6: 629-62 (1988)  1988

      3289580 3289580
      Intrathymic ontogeny of the T cell receptor associated CD3 (T3) antigen.
      Swerdlow, S H, et al.
      Lab. Invest., 58: 421-7 (1988)  1988

      Show Abstract
      2451767 2451767
      The cytoplasmic expression of CD3 antigens in normal and malignant cells of the T lymphoid lineage.
      Campana, D, et al.
      J. Immunol., 138: 648-55 (1987)  1987

      Show Abstract
      3098852 3098852
      The immunocytochemical distribution of leukocytic subpopulations in human endometrium.
      Kamat, B R and Isaacson, P G
      Am. J. Pathol., 127: 66-73 (1987)  1987

      Show Abstract
      3565538 3565538
      APAAP labelling of blood and bone-marrow samples for phenotyping leukaemia.
      Erber, W N, et al.
      Lancet, 1: 761-5 (1986)  1986

      Show Abstract
      2870268 2870268
      Evidence for the T3-associated 90K heterodimer as the T-cell antigen receptor.
      Meuer, S C, et al.
      Nature, 303: 808-10 (1983)  1983

      Show Abstract
      6191218 6191218
      Distinctive functional characteristics of human "T" lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody.
      Beverley, P C and Callard, R E
      Eur. J. Immunol., 11: 329-34 (1981)  1981

      6788570 6788570
      Monoclonal antibodies defining distinctive human T cell surface antigens.
      Kung, P, et al.
      Science, 206: 347-9 (1979)  1979

      Show Abstract
      314668 314668

      Data Sheet

      Title
      Anti-CD3, Clone UCHT1, FITC Conjugated - Data Sheet

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      Alternative Format

      Catalogue Number Description  
      CBL150 Anti-CD3 Antibody, clone UCHT1 Show Pricing & Availability

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies