CBL1502 | Anti-CD45 Antibody, clone MRC OX-1

CBL1502
500 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      RFC, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberCBL1502
      Replaces04-1102
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-CD45 Antibody, clone MRC OX-1
      Alternate Names
      • LCA
      References
      Product Information
      FormatPurified
      PresentationThe monoclonal is presented as 0.5mg at 1mg/mL in phosphate buffered saline containing 10mM sodium azide. We recommend that each laboratory determine an optimum working titre for use in its particular application.
      Applications
      ApplicationThis Anti-CD45 Antibody, clone MRC OX-1 is validated for use in FC, IH for the detection of CD45.
      Key Applications
      • Flow Cytometry
      • Immunohistochemistry
      Application NotesSuitable for flow cytometry

      Suitable for use on frozen sections

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenRat thymocyte membrane glycoproteins
      CloneMRC OX-1
      HostMouse
      SpecificityThe antibody recognises a monomorphic determinant of the rat leucocyte common antigen (LCA, CD45). The antigen recognised is a heavily glycosylated membrane gylcoprotein of M.W. 170kD on thymocytes but M.W. 170- 220kD on other leucocytes (2,3). It is a useful marker for leucocytes as it has not been detected on any other cell type.
      FUSION PARTNER: Spleen cells from BALB/c mice were fused with the cells of the NS1 mouse myeloma cell line.
      IsotypeIgG1
      Species Reactivity
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported.
      Gene Symbol
      • PTPRC
      • L-CA
      • LCA
      • CD45
      • LY5
      • T200
      • glycoprotein
      • B220
      • GP180
      • EC 3.1.3.48
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P08575 # Required for T-cell activation through the antigen receptor. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits an dephosphorylates SKAP1 and FYN.
      SIZE: 1304 amino acids; 147254 Da
      SUBUNIT: Binds GANAB and PRKCSH (By similarity). Interacts with SKAP1.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      DOMAIN: SwissProt: P08575 The first PTPase domain interacts with SKAP1.
      PTM: Heavily N- and O-glycosylated.
      DISEASE: SwissProt: P08575 # Defects in PTPRC are a cause of autosomal recessive severe combined immunodeficiency T-cell-negative/B-cell- positive/NK cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. SCID refers to a genetically and clinically group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients with SCID present in infancy with recurrent, persistent infections by opportunistic organisms, including Candida albicans, Pneumocystis carinii, and cytomegalovirus, among many others. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T- cell development. & Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.
      SIMILARITY: Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily. & Contains 2 fibronectin type-III domains. & Contains 2 tyrosine-protein phosphatase domains.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsFor use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.
      Packaging Information
      Material Size500 µg
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies