05-1413 | Anti-CD45RA Antibody, Clone MEM 56

05-1413
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RFC, IF, IH(P), IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1413
      Replaces04-1102
      DescriptionAnti-CD45RA Antibody, Clone MEM 56
      Alternate Names
      • CD45 antigen
      • Leukocyte common antigen precursor
      • SCID due to PTPRC deficiency
      • T200 glycoprotein
      • T200 leukocyte common antigen
      • Human homolog of severe combined immunodeficiency due to PTPRC deficiency
      • Leukocyte-common antigen
      • Protein tyrosine phosphatase, receptor type C
      • Protein tyrosine phosphatase, receptor type C polypeptide
      Background InformationCD45 is a receptor-tyrosine phosphatase required for antigen-induced signaling in T-cells and B-cells; its substrates are unknown. Interestingly, it associates with several Src-family tyrosine kinases, which are inhibited by tyrosine phosphorylation in their c-termini, suggesting a mechanism for signaling by CD45.
      References
      Product Information
      FormatPurified
      Control
      • RAW264 Cell Lysate
      PresentationPurified mouse monoclonal IgG2b in phosphate buffered saline containing 10 mM sodium azide and 1 mg/mL bovine serum albumin.
      We recommend that each laboratory determine an optimum working titre for use in its particular application.
      Applications
      ApplicationAnti-CD45RA Antibody, Clone MEM 56 detects level of CD45RA & has been published & validated for use in FC, IF, IH(P), IP & WB.
      Key Applications
      • Flow Cytometry
      • Immunofluorescence
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunofluorescence: Identification of CD4+ suppressor/inducer cells, by indirect immunofluorescence staining.

      Immunoprecipitation: A previous lot of this antibody detected 220 kDa molecular weight component of the leucocyte common antigen complex in immunoprecipitation.

      Flow Cytometry: Purification or depletion of CD4+ suppressor/inducer cells by cell sorting or the panning techniques was performed on a previous lot.

      Immunohistochemistry(paraffin): Representative testing from a previous lot.
      Identification of CD45R+ lymphocytes in cryostat sections of frozen tissue and in formalin fixed wax embedded tissue.

      Optimal Staining of CD45RA Monoclonal Antibody: Tonsil
      Biological Information
      CloneMEM 56
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes the 220 kDa molecular weight component of the leucocyte common antigen complex expressed on some CD4+ T lymphocytes, B lymphocytes and CD8+ lymphocytes. The CD45R+/CD4+ lymphocytes functionally exert suppressor/inducer activity in in vitro assay systems.
      Antigen distribution:
      B-cells (lg+) > 95%
      T-cells (E+) 70 + 10%
      Peripheral blood lymphocytes 68 + 8%
      T-cells (CD4+) 50 + 10%
      Monocytes 50 + 20%
      Thymocytes < 5%
      Granulocytes < 1%
      IsotypeIgG2b
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteMouse. Expected to react with human and rat based on sequence homology. .
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported.
      Gene Symbol
      • PTPRC
      • L-CA
      • LCA
      • CD45
      • LY5
      • T200
      • Glycoprotein
      • B220
      • GP180
      • EC 3.1.3.48
      Purification MethodPurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P08575 # Required for T-cell activation through the antigen receptor. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits an dephosphorylates SKAP1 and FYN.
      SIZE: 1304 amino acids; 147254 Da
      SUBUNIT: Binds GANAB and PRKCSH (By similarity). Interacts with SKAP1.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      DOMAIN: SwissProt: P08575 The first PTPase domain interacts with SKAP1.
      PTM: Heavily N- and O-glycosylated.
      DISEASE: SwissProt: P08575 # Defects in PTPRC are a cause of autosomal recessive severe combined immunodeficiency T-cell-negative/B-cell- positive/NK cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. SCID refers to a genetically and clinically group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients with SCID present in infancy with recurrent, persistent infections by opportunistic organisms, including Candida albicans, Pneumocystis carinii, and cytomegalovirus, among many others. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T- cell development. & Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.
      SIMILARITY: Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily. & Contains 2 fibronectin type-III domains. & Contains 2 tyrosine-protein phosphatase domains.
      Molecular Weight~220 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on RAW 264 lysates.

      Western Blot Analysis: 1:500 dilution of this lot detected CD45RA on 10 μg of RAW 264
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsFor use within 1 month of date of purchase store at 2-8°C, for long term storage aliquot antibody into small volumes and store at -20°C for up to 1 year from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-CD45RA, Clone MEM 56 - NG1576287 NG1576287
      Anti-CD45RA, Clone MEM 56 - NG1631264 NG1631264
      Anti-CD45RA, clone MEM 56 - 2192169 2192169
      Anti-CD45RA, clone MEM 56 - 2227163 2227163
      Anti-CD45RA, clone MEM 56 - 2326997 2326997
      Anti-CD45RA, clone MEM 56 - 2495669 2495669
      Anti-CD45RA, clone MEM 56 - NG1853758 NG1853758
      Anti-CD45RA, clone MEM 56 - NG1898622 NG1898622
      Anti-CD45RA, clone MEM 56 -2682370 2682370

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies