CBL180F-I | Anti-CD62E/CD62P Antibody, clone 1.2B6, FITC Conjugate

CBL180F-I
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIF, FCMFITCMonoclonal Antibody
      Description
      Catalogue NumberCBL180F-I
      DescriptionAnti-CD62E/CD62P Antibody, clone 1.2B6, FITC Conjugate
      Alternate Names
      • E-selectin
      • CD62 antigen-like family member E
      • CD62E
      • ELAM-1
      • Endothelial adhesion molecule 1
      • Endothelial leukocyte adhesion molecule 1
      • Leukocyte-endothelial cell adhesion molecule 2
      • P-selectin
      • CD62 antigen-like family member P
      • CD62P
      • GMP-140
      • Granule membrane protein 140
      • Granulocyte membrane protein
      • LECAM3
      • Leukocyte-endothelial cell adhesion molecule 3
      • PADGEM
      • Platelet activation dependent granule-external membrane protein
      • Platelet alpha-granule membrane protein
      Background InformationSelectins (CD62s) are single-chain transmembrane glycoproteins that function as cell adhesion molecules (CAMs) by interacting with a range of oligosaccharide structures including sialyl-Lewis X (sLeX) via their C-type lectin domain. All three known selectins (L-, E-, and P-selectin or CD62L CD62E, CD62P, respectively) share a similar overall domain structure that includes an N-terminal C-type lectin domain (calcium-dependent lectin domain), an EGF-like domain, a variable number of short consensus repeat (SCR) or Sushi domains (2, 6, and 9 for CD62L CD62E, CD62P, respectively), a transmembrane domain, and a cytoplasmic tail. The extracellular domains show a high homology among the three selectins, the transmembrane and cytoplasmic domains however are not conserved among the three. E-selectin (CD62E, ELAM-1) and P-selectin (CD62P, GMP-140, LECAM3, PADGEM) are mainly expressed on endothelial cells at inflammatory sites, whereas L-selectin (CD62L) is exclusively expressed on leukocytes.
      References
      Product Information
      FormatFITC
      PresentationPurified mouse monoclonal IgG1κ antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
      Applications
      ApplicationAnti-CD62E/CD62P Antibody, clone 1.2B6, FITC Conjugate is an antibody against CD62E/CD62P for use in Immunofluorescence, Flow Cytometry.
      Key Applications
      • Immunofluorescence
      • Flow Cytometry
      Application NotesFlow Cytometry Analysis: A representative lot detected E-selectin (CD62E) expression induction on the surface of human umbilical vein endothelial cells (HUVECs) following stimuation with a trimeric form of the murine CD40 ligand, CD40LT (Karmann, K., et al. (1995). Proc. Natl. Acad. Sci. U. S. A. 92(10):4342-4346).
      The unconjugated antibody (Cat. Nos. CBL180 & CBL180-I) is shown to be suitable also for ELISA, flow cytometry, immunohistochemistry, immunoprecipitation, neutralization, and Western blotting applications.
      Biological Information
      ImmunogenTNF-activated human umbilical vein endothelial cells.
      EpitopeC-type lectin domain.
      Clone1.2B6
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityThe antibody is specific to human antigen E-selectin (ELAM-1, 115 kDa) and P-selectin that are expressed on activated endothelial cells. The ligand is expressed on neutrophils and may function as an early part in neutrophil binding to the endothelium. The antibody inhibits neutrophil adhesion to E-selectin and P-selectin when used as F(ab')2 fragments. Although previously thought to recognize only human CD62E, later data (Goda, K., et al., (1999). Eur. J. Immunol. 29(5):1551-1560) shows that this antibody also recognizes human CD62P binding to a common epitope on these members of the selectin family.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SELE
      • ELAM
      • ELAM1
      • ESEL
      • SELPGMRP
      • GMP140
      • GRMP
      • PSEL
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight65.51 kDa (CD62E) and 89.70 kDa (CD62P) calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunofluorescence in human tonsil tissue.

      Immunofluorescence Analysis: A 1:50 dilution of this antibody detected CD62E/CD62P in human tonsil tissue sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications