CBL1507 | Anti-CD8 α Chain Antibody, clone OX-8

CBL1507
500 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      RFC, IHC, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberCBL1507
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-CD8 α Chain Antibody, clone OX-8
      Alternate Names
      • T-lymphocyte Differentiation Antigen T8/Leu-2
      References
      Product Information
      FormatPurified
      PresentationThe monoclonal is presented as 0.5mg in phosphate buffered saline containing 10mM sodium azide. We recommend that each laboratory determine an optimum working titre for use in its particular application.
      Applications
      ApplicationAnti-CD8 α Chain Antibody, clone OX-8 is an antibody against CD8 α Chain for use in FC, IH, IH(P).
      Key Applications
      • Flow Cytometry
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      Application NotesThe study of phenotypes of T cells mediating various T cell functions.

      Suitable for use on frozen tissue sections and on formalin -fixed paraffin wax embedded tissues.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      CloneOX-8
      HostMouse
      SpecificityThis antibody detects the CD8 antigen present on a subset of T cells that has suppressor and cytotoxic activities. 90% of thymocytes and NK cells are labelled with this antigen.
      IsotypeIgG1
      Species Reactivity
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain isoforms. Two alternative transcripts encoding distinct isoforms, one membrane associated and one secreted, have been identified.
      Gene Symbol
      • CD8A
      • p32
      • CD8
      • MAL
      • Leu2
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P01732 # Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
      SIZE: 235 amino acids; 25729 Da
      SUBUNIT: In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers. Shown to be expressed as heterdimer on thymocytes and as homodimer on peripheral blood T-lymphocytes. Interacts with the MHC class I HLA-A/B2M dimer. Interacts with LCK in a zinc-dependent manner.
      SUBCELLULAR LOCATION: Isoform 1: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted.
      PTM: All of the five most carboxyl-terminal cysteines form inter- chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not (By similarity).
      DISEASE: SwissProt: P01732 # Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
      SIMILARITY: SwissProt: P01732 ## Contains 1 Ig-like V-type (immunoglobulin-like) domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsFor use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.
      Packaging Information
      Material Size500 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-RAT CD8 alpha CHAIN MONOCLONAL ANTIBODY - 2142161 2142161
      MOUSE ANTI-RAT CD8 alpha CHAIN MONOCLONAL ANTIBODY - 2383946 2383946
      MOUSE ANTI-RAT CD8 alpha CHAIN MONOCLONAL ANTIBODY - 1979257 1979257
      MOUSE ANTI-RAT CD8 alpha CHAIN MONOCLONAL ANTIBODY - 2090425 2090425
      MOUSE ANTI-RAT CD8 alpha CHAIN MONOCLONAL ANTIBODY - 2318753 2318753

      References

      Reference overviewPub Med ID
      A dual-label technique for the immunohistochemical demonstration of T-lymphocyte subsets in formalin-fixed, paraffin-embedded rat lymphoid tissue.
      Randall, KJ; Pearse, G
      Toxicologic pathology  36  795-804  2008

      Show Abstract
      18776164 18776164
      Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies.
      Whiteland, J L, et al.
      J. Histochem. Cytochem., 43: 313-20 (1995)  1995

      Show Abstract
      7868861 7868861
      Striking similarities between antigen receptor J pieces and sequence in the second chain of the murine CD8 antigen.
      Johnson, P and Williams, A F
      Nature, 323: 74-6 (1986)  1986

      Show Abstract
      3092111 3092111
      The roles of host and donor cells in the rejection of skin allografts by T cell-deprived rats injected with syngeneic T cells.
      Dallman, M J, et al.
      Eur. J. Immunol., 12: 511-8 (1982)  1982

      6749527 6749527
      Phenotype of rat natural killer cells defined by monoclonal antibodies marking rat lymphocyte subsets.
      Cantrell, D A, et al.
      Immunology, 45: 97-103 (1982)  1982

      Show Abstract
      7056570 7056570
      Subsets of T cells in the rat mediating lethal graft versus-host disease.
      Mason, D W
      Transplantation, 32: 222-6 (1981)  1981

      6974419 6974419
      Analysis of cell surfaces by xenogeneic myeloma-hybrid antibodies: differentiation antigens of rat lymphocytes.
      Williams, A F, et al.
      Cell, 12: 663-73 (1977)  1977

      303545 303545

      Data Sheet

      Title
      MOUSE ANTI-RAT CD8 alpha CHAIN MONOCLONAL ANTIBODY

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies