06-1295 | Anti-CDT1 Antibody

06-1295
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, MWBRbSerumPolyclonal Antibody
      Description
      Catalogue Number06-1295
      DescriptionAnti-CDT1 Antibody
      Alternate Names
      • DNA replication factor
      • Double parked homolog
      • Double parked, Drosophila, homolog of
      • chromatin licensing and DNA replication factor 1
      Background InformationCDT1 (Chromatin Licensing and DNA replication Factor 1) is an essential component of the cell cycle machinery. It plays an important role in insuring that there is only one round of DNA replication per cell cycle. CDT1 is a known substrate of Cdk2 and Cdk4 (Liu, E. 2004). It is presented in G1 of the cell cycle and is degraded as cells enter S phase (Liu, E. 2004). In higher eukaryotes, CDT1 is known to interact with Geminin, a protein that is degraded during mitosis, that inhibits it. CDT1 is marked for degradation by two E3 ligases: SCF complex and the CUL4-DDB1 complex (Sansam, 2006).
      References
      Product Information
      FormatSerum
      Control
      • HeLa cell lysate
      PresentationRabbit polyclonal serum with 0.05% sodium azide.
      Applications
      ApplicationUse Anti-CDT1 Antibody (Rabbit Polyclonal Antibody) validated in WB to detect CDT1 also known as DNA replication factor, Double parked homolog.
      Key Applications
      • Western Blotting
      Application NotesWestern Blot Analysis: A 1:1,000 dilution from a previous lot detected CDT1 in C2C12, Hek293, HeLa, HepG2, Huvec, Jurkat, L6, mouse brain, NIH/3T3, PC3, and RAW264.7 cell lysates.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human CDT1.
      HostRabbit
      SpecificityThis antibody recognizes CDT1.
      Species Reactivity
      • Human
      • Rat
      • Mouse
      Species Reactivity NoteProven to react with human, mouse and rat.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • DUP
      • RIS2
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: Cooperates with CDC6 to promote the loading of the mini-chromosome maintenance complex onto chromatin to form the pre-replication complex necessary to initiate DNA replication. Binds DNA in a sequence-, strand-, and conformation-independent manner. Potential oncogene.
      SUBUNIT STRUCTURE: Interaction with GMNN inhibits both binding of the MCM complex to origins of replication and DNA-binding activity.
      SUBCELLULAR LOCATION: Nucleus.
      DEVELOPMENTAL STAGE: Present during G1 and early S phase of the cell cycle. Degraded during the late S, G2, and M phases.
      PTM : Ubiquitinated, leading to its subsequent degradation. The interaction with GMNN protects it against ubiquitination.
      Phosphorylated by cyclin A-dependent kinases which results in the binding of CDT1 to the F-box protein SKP2 and subsequent degradation. Binding to GMNN is not affected by phosphorylation.
      SEQUENCE SIMILARITIES: Belongs to the Cdt1 family.
      SEQUENCE CAUTION: The sequence AF070552 differs from that shown. Reason: Frameshift at positions 278 and 312.
      Molecular Weight~60 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.
      Western Blot Analysis: A 1:1,000 dilution of this antibody detected CDT1 in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-CDT1 - 2166819 2166819
      Anti-CDT1 - 2216889 2216889
      Anti-CDT1 - NG1873205 NG1873205
      Anti-CDT1 - NRG1633608 NRG1633608
      Anti-CDT1 -2686903 2686903
      Anti-CDT1 Polyclonal Antibody 2975294

      References

      Reference overviewPub Med ID
      Efficient parvovirus replication requires CRL4Cdt2-targeted depletion of p21 to prevent its inhibitory interaction with PCNA.
      Adeyemi, RO; Fuller, MS; Pintel, DJ
      PLoS pathogens  10  e1004055  2014

      Show Abstract
      24699724 24699724
      A gatekeeper residue for NEDD8-activating enzyme inhibition by MLN4924.
      Toth, JI; Yang, L; Dahl, R; Petroski, MD
      Cell reports  1  309-16  2012

      Show Abstract
      22832224 22832224
      Minichromosome maintenance helicase paralog MCM9 is dispensible for DNA replication but functions in germ-line stem cells and tumor suppression.
      Hartford, SA; Luo, Y; Southard, TL; Min, IM; Lis, JT; Schimenti, JC
      Proceedings of the National Academy of Sciences of the United States of America  108  17702-7  2011

      Show Abstract
      21987787 21987787

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies