07-1383 | Anti-CDT1 Antibody

07-1383
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, IPRbSerumPolyclonal Antibody
      Description
      Catalogue Number07-1383
      DescriptionAnti-CDT1 Antibody
      Alternate Names
      • DNA replication factor
      • Double parked homolog
      • Double parked, Drosophila, homolog of 2 chromatin licensing and DNA replication factor 1
      Background InformationCDT1 (Chromatin Licensing and DNA replication Factor 1) is an essential component of the cell cycle machinery. It plays an important role in insuring that there is only one round of DNA replication per cell cycle. CDT1 is a known substrate of Cdk2 and Cdk4 (Liu, E. 2004). It is presented in G1 of the cell cycle and is degraded as cells enter S phase (Liu, E. 2004). In higher eukaryotes, CDT1 is known to interact with Geminin, a protein that is degraded during mitosis, that inhibits it. CDT1 is marked for degradation by two E3 ligases: SCF complex and the CUL4-DDB1 complex (Sansam, 2006).
      References
      Product Information
      FormatSerum
      PresentationUnpurified rabbit serum containing 0.05% NaN3.
      Applications
      ApplicationDetect CDT1 using this Anti-CDT1 Antibody validated for use in WB & IP.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesAn independent lab detected this antibody to immunoprecipitate (IP) Cdt1 using 2 μL/IP reaction (Wohlschlegel, B.T., 2000).
      Biological Information
      ImmunogenRecombinant protein including amino acids 238-546 of human CDT1.
      EpitopeC-terminal half
      HostRabbit
      SpecificityThis antibody recognizes the Cdt1.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • DUP
      • RIS2
      UniProt Number
      UniProt SummaryFUNCTION: Cooperates with CDC6 to promote the loading of the mini-chromosome maintenance complex onto chromatin to form the pre-replication complex necessary to initiate DNA replication. Binds DNA in a sequence-, strand-, and conformation-independent manner. Potential oncogene. UniProtKB Q8R4E9

      SUBUNIT STRUCTURE: Interaction with GMNN inhibits both binding of the MCM complex to origins of replication and DNA-binding activity. UniProtKB Q8R4E9

      SUBCELLULAR LOCATION: Nucleus.

      DEVELOPMENTAL STAGE: Present during G1 and early S phase of the cell cycle. Degraded during the late S, G2, and M phases.

      PTM: Ubiquitinated, leading to its subsequent degradation. The interaction with GMNN protects it against ubiquitination.

      Phosphorylated by cyclin A-dependent kinases which results in the binding of CDT1 to the F-box protein SKP2 and subsequent degradation. Binding to GMNN is not affected by phosphorylation.

      SEQUENCE SIMILARITIES: Belongs to the Cdt1 family.

      SEQUENCE CAUTION: The sequence AF070552 differs from that shown. Reason: Frameshift at positions 278 and 312.

      Molecular Weight65 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by western blot on Jurkat cell lysate.

      Western Blot Analysis: 1:1,000 dilution of this antibody was used to detect CDT1 in Jurkat cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 2 years at -20°C from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-CDT1 2476750
      Anti-CDT1 - 2051947 2051947
      Anti-CDT1 - NG1618263 NG1618263
      Anti-CDT1 - NG1806854 NG1806854
      Anti-CDT1 -2673041 2673041
      Anti-CDT1 Polyclonal Antibody 2964471
      Anti-CDT1 Polyclonal Antibody 2970628
      Anti-CDT1 Polyclonal Antibody 3089158

      References

      Reference overviewPub Med ID
      Human papillomavirus E7 induces rereplication in response to DNA damage.
      Fan, X; Liu, Y; Heilman, SA; Chen, JJ
      Journal of virology  87  1200-10  2013

      Show Abstract
      23152514 23152514

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies