04-205 | Anti-CENP-A Antibody, clone EP800Y, rabbit monoclonal

04-205
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RFC, ICC, IHC, IH(P), WBRbUnpurifiedMonoclonal Antibody
      Description
      Catalogue Number04-205
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-CENP-A Antibody, clone EP800Y, rabbit monoclonal
      Alternate Names
      • Centromere autoantigen A
      • centromere protein A
      • centromere protein A (17kD)
      • centromere protein A, 17kDa
      Background InformationCentromere protein A (CENP-A) is a 17 kDa centromere-specific histone variant with 62% amino acids homology to the C-terminal of histone H3. Localized in the centromere, it plays a central role in the centromere-specific chromatin formation. The depletion of histone H3 at the CENP-A binding domain suggests CENP-A to be a possible replacement for histone H3 in the packaging process of α-satellite DNA into primary chromatin structure. CENP-A is essential in the formation of specialized nucleosomes at the centromere, implicating CENP-A as a centromere-specific epigenetic marker.
      References
      Product Information
      FormatUnpurified
      Control
      • HeLa Whole Cell lysate.
      PresentationUnpurified rabbit monoclonal IgG in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA
      Applications
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesFlow Cytometry:
      1:80 dilution of a previous lot was used for flow cytometry.

      Immunocytochemistry:
      1:250 – 500 dilution of a previous lot was used for Immunocytochemistry.

      Immunohistochemistry:
      1:250 – 500 dilution of a previous lot was used for Immunohistochemistry.

      Immunocytochemistry:
      A previous lot was used to detect CENP-A in HeLa cells (1:250 dilution).


      Biological Information
      ImmunogenSynthetic peptide corresponding to the C-terminal region of CENP-A, conjugated to KLH.
      EpitopeC-terminus
      CloneEP800Y
      HostRabbit
      SpecificityThis antibody recognizes the C-terminal region of human CENP-A.
      IsotypeIgG
      Species Reactivity
      • Human
      • Rat
      Species Reactivity NoteProven to react with human and rat CENP-A. Does not react with mouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryCentromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq]
      Gene Symbol
      • CENP-A
      • OTTHUMP00000122589
      Non-Reactive Species
      • Mouse
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division.

      SUBUNIT: Forms a nucleosome-like histone octamer containing two molecules each of H2A, H2B, CENPA and H4 assembled in one CENPA-H4 heterotetramer and two H2A-H2B heterodimers. Nucleosomes containing CENPA also contain histone H2A variants such as macroH2A H2AFY and H2A.Z/H2AFZ. The CENPA-H4 heterotetramer is more compact and structurally more rigid than corresponding H3-H4 heterotetramers. Component of the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and MLF1IP/CENPU. Interacts directly with herpes virus HSV-1 ICP0 protein. Ref.5 Ref.7 Ref.12

      SUBCELLULAR LOCATION: Nucleus. Centromere. Kinetochore. Note= Localizes exclusively in the kinetochore domain of centromeres. Ref.10 Ref.11

      DOMAIN: The CATD (CENPA targeting domain) region is responsible for the more compact structure of nucleosomes containing CENPA and is necessary and sufficient to mediate the localization into centromeres. Ref.12

      PTM: Ubiquitinated Probable. Interaction with herpes virus HSV-1 ICP0 protein, leads to its degradation by the proteasome pathway.

      Phosphorylation of Ser-7 by Aurora-A/STK6 and Aurora-B/STK12 during prophase is required for localization of Aurora-A/STK6 and Aurora-B/STK12 at inner centromere and is essential for kinetochore function. Initial phosphorylation during prophase is mediated by Aurora-A/STK6 and is maintained by Aurora-B/STK12.

      DISEASE:Antibodies against CENPA are present in sera from patients with autoimmune diseases that developed autoantibodies against centrosomal proteins. Ref.6

      SIMILARITY: Belongs to the histone H3 family.


      Molecular Weight18 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on HeLa Whole Cell lysate.

      Western Blot Analysis:
      A 1:5,000 dilution of this lot was used to detect CENP-A HeLa Whole Cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-CENP-A, clone EP800Y - 2437708 2437708
      Anti-CENP-A, clone EP800Y - 2445820 2445820
      Anti-CENP-A, clone EP800Y - 2197147 2197147
      Anti-CENP-A, clone EP800Y - 2276281 2276281
      Anti-CENP-A, clone EP800Y - NG1584114 NG1584114

      References

      Reference overviewApplicationPub Med ID
      Involvement of telomerase reverse transcriptase in heterochromatin maintenance.
      Maida, Y; Yasukawa, M; Okamoto, N; Ohka, S; Kinoshita, K; Totoki, Y; Ito, TK; Minamino, T; Nakamura, H; Yamaguchi, S; Shibata, T; Masutomi, K
      Molecular and cellular biology  34  1576-93  2014

      Show Abstract
      Western Blotting24550003 24550003
      The histone variant CENP-A and centromere specification.
      Black, Ben E and Bassett, Emily A
      Curr. Opin. Cell Biol., 20: 91-100 (2008)  2008

      18226513 18226513
      Heterochromatin tells CENP-A where to go.
      Durand-Dubief, Mickaël and Ekwall, Karl
      Bioessays, 30: 526-9 (2008)  2008

      Show Abstract
      18478529 18478529
      A 17-kD centromere protein (CENP-A) copurifies with nucleosome core particles and with histones.
      Palmer, D K, et al.
      J. Cell Biol., 104: 805-15 (1987)  1987

      3558482 3558482