05-583 | Anti-CFTR Antibody, clone M3A7

05-583
200 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIHC, IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-583
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-CFTR Antibody, clone M3A7
      Alternate Names
      • ATP-binding cassette sub-family C, member 7
      • ATP-binding cassette transporter sub-family C member 7
      • cAMP-dependent chloride channel
      • cystic fibrosis transmembrane conductance regulator
      • cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7)
      • cystic fibrosis transmembrane conductance regulator
      • ATP-binding cassette (sub-family C, member 7)
      Background InformationCFTR (Cystic Fibrosis Transmembrane Regulator) is an integral membrane protein shown to be involved in the transport of chloride ions. It is found on the surface of the epithelial cells lining the lungs and other organs. CFTR defects are the cause of cystic fibrosis. Cystic Fibrosis (CF) is a common lethal genetic disease caused by mutations of the gene coding for the cystic fibrosis transmembrane conductance factor, a cAMP regulated chloride channel. Approximately 70% of all CF cases share the deletion of a phenylalanine at position 508 (delta F508), which results in abnormal chloride transport. Since the CF mutation is lethal, most often by lung and liver disease, it raises the question of why this genetic disease remains as common as it is.
      References
      Product Information
      FormatPurified
      Control
      • T84 cell lysate.
      PresentationPurified mouse monoclonal IgG1 in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide and 30% glycerol.
      Applications
      ApplicationDetect CFTR using this Anti-CFTR Antibody, clone M3A7 validated for use in IH, IP & WB.
      Key Applications
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation:
      This antibody has been reported to immunoprecipitate CFTR. (Kartner, N., 1998.)

      Immunohistochemistry:
      This antibody has been reported to immunostain CFTR in human pancreatic tissue sections. (Kartner, N., 1998.)
      Biological Information
      ImmunogenGST-fusion protein corresponding to residues 1197-1480 of human cystic fibrosis transmembrane conductance regulator (CFTR). The epitope maps within amino acids 1365-1395. Clone M3A7.
      Epitopea.a. 1365-1395
      CloneM3A7
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes CFTR, Mr 170 kDa.
      IsotypeIgG1
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Other species not tested.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the ATP-binding cassette (ABC) transporter superfamily. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily that is involved in multi-drug resistance. The encoded protein functions as a chloride channel and controls the regulation of other transport pathways. Mutations in this gene are associated with the autosomal recessive disorders cystic fibrosis and congenital bilateral aplasia of the vas deferens. Alternatively spliced transcript variants have been described, many of which result from mutations in this gene.
      Gene Symbol
      • CFTR
      • CFTR/MRP
      • ABCC7
      • CBAVD
      • dJ760C5.1
      • CF
      • MRP7
      • ABC35
      • TNR-CFTR
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P13569 # Involved in the transport of chloride ions. May regulate bicarbonate secretion and salvage in epithelial cells by regulating the SLC4A7 transporter.
      SIZE: 1480 amino acids; 168142 Da
      SUBUNIT: Interacts with SHANK2 (By similarity). Interacts with SLC9A3R1, MYO6 and GOPC. Interacts with SLC4A7 through SLC9A3R1.
      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
      TISSUE SPECIFICITY: Found on the surface of the epithelial cells that line the lungs and other organs.
      DOMAIN: SwissProt: P13569 The PDZ-binding motif mediates interactions with GOPC and with the SLC4A7, SLC9A3R1/EBP50 complex.
      DISEASE: SwissProt: P13569 # Defects in CFTR are the cause of cystic fibrosis (CF) [MIM:219700]; also known as mucoviscidosis. CF is the most common genetic disease in the Caucasian population, with a prevalence of about 1 in 2000 live births. Inheritance is autosomal recessive. CF is a common generalized disorder of exocrine gland function which impairs clearance of secretions in a variety of organs. It is characterized by the triad of chronic bronchopulmonary disease (with recurrent respiratory infections), pancreatic insufficiency (which leads to malabsorption and growth retardation) and elevated sweat electrolytes. & Defects in CFTR are the cause of congenital bilateral absence of the vas deferens (CBAVD) [MIM:277180]. CBAVD is an important cause of sterility in men and could represent an incomplete form of cystic fibrosis, as the majority of men suffering from cystic fibrosis lack the vas deferens.
      SIMILARITY: Belongs to the ABC transporter family. CFTR transporter (TC 3.A.1.202) subfamily. & Contains 2 ABC transmembrane type-1 domains. & Contains 2 ABC transporter domains.
      Molecular Weight170 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot on human T84 colon carcinoma epithelial RIPA cell lysates or from CFTR-transfected BHK2.

      Western Blot Analysis:
      0.5-2 µg/mL of this lot detected CFTR from 20-50 µg of human T84 colon carcinoma epithelial RIPA cell lysates. 0.5-2 µg/mL of a previous lot detected CFTR from CFTR-transfected BHK (Haardt, M., 1999).
      Note: Do not boil the lysate. Instead incubate at 37°C for 30 minutes. CFTR can run as a diffuse protein on SDS-PAGE.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations:
      Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-CFTR, clone M3A7 (mouse monoclonal IgG1) - DAM1394766 DAM1394766
      Anti-CFTR, clone M3A7 (mouse monoclonal IgG1) - DAM1472180 DAM1472180
      Anti-CFTR, clone M3A7 - 2384714 2384714
      Anti-CFTR, clone M3A7 - 2441947 2441947
      Anti-CFTR, clone M3A7 - 1963621 1963621
      Anti-CFTR, clone M3A7 - 1990753 1990753
      Anti-CFTR, clone M3A7 - 20169 20169
      Anti-CFTR, clone M3A7 - 2021756 2021756
      Anti-CFTR, clone M3A7 - 2068173 2068173
      Anti-CFTR, clone M3A7 - 21640 21640

      References

      Reference overviewApplicationPub Med ID
      Mechanosensitive Cl- secretion in biliary epithelium mediated through TMEM16A.
      Dutta, AK; Woo, K; Khimji, AK; Kresge, C; Feranchak, AP
      American journal of physiology. Gastrointestinal and liver physiology  304  G87-98  2013

      Show Abstract
      23104560 23104560
      The GAP portion of Pseudomonas aeruginosa type III secreted toxin ExoS upregulates total and surface levels of wild type CFTR.
      Tukaye, DN; Kwon, SH; Guggino, WB
      Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology  31  153-65  2013

      Show Abstract
      23428533 23428533
      Transforming growth factor-β1 impairs CFTR-mediated anion secretion across cultured porcine vas deferens epithelial monolayer via the p38 MAPK pathway.
      Yi, S; Pierucci-Alves, F; Schultz, BD
      American journal of physiology. Cell physiology  305  C867-76  2013

      Show Abstract
      Western Blotting23903699 23903699
      CFTR is involved in the fine tuning of intracellular redox status: physiological implications in cystic fibrosis.
      Duranton, Christophe, et al.
      Am. J. Pathol., 181: 1367-77 (2012)  2012

      Show Abstract
      22846720 22846720
      CFTR mediates apoptotic volume decrease and cell death by controlling glutathione efflux and ROS production in cultured mice proximal tubules.
      Sebastien l'Hoste,Abderrahmen Chargui,Radia Belfodil,Elisabeth Corcelle,Christophe Duranton,Isabelle Rubera,Chantal Poujeol,Baharia Mograbi,Michel Tauc,Philippe Poujeol
      American journal of physiology. Renal physiology  298  2010

      Show Abstract
      19906953 19906953
      A mutation in the cystic fibrosis transmembrane conductance regulator generates a novel internalization sequence and enhances endocytic rates♪Reelin-mediated signaling locally regulates protein kinase B/Akt and glycogen synthase kinase 3beta♪The co-acti
      Silvis, M. R., et al
      J Biol Chem, 278:11554-60 (2003)  2003

      Immunoblotting (Western)12529365 12529365
      Mammalian Osmolytes and S-Nitrosoglutathione Promote {Delta}F508 Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) Protein Maturation and Function
      Howard, M., et al
      J Biol Chem, 278:35159-35167 (2003)  2003

      Immunoblotting (Western), Immunoprecipitation12837761 12837761
      Reactive oxygen nitrogen species decrease cystic fibrosis transmembrane conductance regulator expression and cAMP-mediated Cl- secretion in airway epithelia♪A mutation in the cystic fibrosis transmembrane conductance regulator generates a novel internali
      Bebok, Z., et al
      J Biol Chem, 277:43041-9 (2002)  2002

      Immunoblotting (Western)12167629 12167629
      C-terminal truncations destabilize the cystic fibrosis transmembrane conductance regulator without impairing its biogenesis. A novel class of mutation.
      Haardt, M, et al.
      J. Biol. Chem., 274: 21873-7 (1999)  1999

      Show Abstract
      10419506 10419506
      Characterization of polyclonal and monoclonal antibodies to cystic fibrosis transmembrane conductance regulator.
      Kartner, N and Riordan, J R
      Meth. Enzymol., 292: 629-52 (1998)  1998

      9711588 9711588

      Related Products & Applications

      Related Products

      Catalogue Number Description  
      05-581 Anti-CFTR Antibody, clone MM13-4 Show Pricing & Availability
      MAB3480 Anti-CFTR Antibody, a.a. 1370-1380, clone M3A7 Show Pricing & Availability
      MAB3482 Anti-Cystic Fibrosis Transmembrane Conductance Regulator Antibody, clone MM13-4 Show Pricing & Availability
      MAB3484 Anti-Cystic Fibrosis Transmembrane Conductance Regulator Antibody, a.a. 386-412, clone L12B4 Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies