05-581 | Anti-CFTR Antibody, clone MM13-4

05-581
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICC, IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-581
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-CFTR Antibody, clone MM13-4
      Alternate Names
      • ATP-binding cassette sub-family C, member 7
      • ATP-binding cassette transporter sub-family C member 7
      • cAMP-dependent chloride channel
      • cystic fibrosis transmembrane conductance regulator
      • cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7)
      • cystic fibrosis transmembrane conductance regulator
      • ATP-binding cassette (sub-family C, member 7)
      Background InformationCFTR (Cystic Fibrosis Transmembrane conductance Regulator) is a 170 kDa chloride (Cl-) channel that regulates salt and water transport across epithelial tissues, where it modulates production of mucus, sweat, saliva, tears, and digestive enzymes. Cystic fibrosis patients have mutations in this protein, resulting in defective in ion transport. Symptoms include reduced transport of sodium chloride (saltier sweat) and digestive and respiratory disorders caused by thicker mucus. CFTR mutations are also associated with congenital bilateral absence of the vas deferens in males.
      References
      Product Information
      FormatPurified
      Control
      • Pancreas. RIPA cell lysates
      PresentationPurified mouse monoclonal IgG1k in buffer containing 0.02M phosphate buffer, pH 7.6, 0.25M NaCl, and 0.1% sodium azide.
      Applications
      ApplicationAnti-CFTR Antibody, clone MM13-4 detects level of CFTR & has been published & validated for use in IC, IP & WB.
      Key Applications
      • Immunocytochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation:
      This antibody has been shown to immunoprecipitate CFTR from a T84 cell lysate by an independent laboratory.


      Immunocytochemistry:
      This antibody has been reported to immunostain CFTR in T84 cells.
      Biological Information
      ImmunogenSynthetic peptide (RKGYRQRLELSD) corresponding to residues 25-36 of human cystic fibrosis transmembrane conductance regulator (CFTR). The immunizing sequence is identical in monkey and rabbit, has 11/12 identical amino acids in sheep, cow and frog, 10/12 identity in mouse and 9/12 identity in rat. Clone MM13-4.
      CloneMM13-4
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes CFTR, Mr 170kDa. Two additional proteins were detected, Mr 52 and 38 kDa, in CFTR-transfected BHK cell lysates.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman, other species cross-reactivity not tested.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the ATP-binding cassette (ABC) transporter superfamily. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily that is involved in multi-drug resistance. The encoded protein functions as a chloride channel and controls the regulation of other transport pathways. Mutations in this gene are associated with the autosomal recessive disorders cystic fibrosis and congenital bilateral aplasia of the vas deferens. Alternatively spliced transcript variants have been described, many of which result from mutations in this gene.
      Gene Symbol
      • CFTR
      • CFTR/MRP
      • ABCC7
      • CBAVD
      • dJ760C5.1
      • CF
      • MRP7
      • ABC35
      • TNR-CFTR
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P13569 # Involved in the transport of chloride ions. May regulate bicarbonate secretion and salvage in epithelial cells by regulating the SLC4A7 transporter.
      SIZE: 1480 amino acids; 168142 Da
      SUBUNIT: Interacts with SHANK2 (By similarity). Interacts with SLC9A3R1, MYO6 and GOPC. Interacts with SLC4A7 through SLC9A3R1.
      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
      TISSUE SPECIFICITY: Found on the surface of the epithelial cells that line the lungs and other organs.
      DOMAIN: SwissProt: P13569 The PDZ-binding motif mediates interactions with GOPC and with the SLC4A7, SLC9A3R1/EBP50 complex.
      DISEASE: SwissProt: P13569 # Defects in CFTR are the cause of cystic fibrosis (CF) [MIM:219700]; also known as mucoviscidosis. CF is the most common genetic disease in the Caucasian population, with a prevalence of about 1 in 2000 live births. Inheritance is autosomal recessive. CF is a common generalized disorder of exocrine gland function which impairs clearance of secretions in a variety of organs. It is characterized by the triad of chronic bronchopulmonary disease (with recurrent respiratory infections), pancreatic insufficiency (which leads to malabsorption and growth retardation) and elevated sweat electrolytes. & Defects in CFTR are the cause of congenital bilateral absence of the vas deferens (CBAVD) [MIM:277180]. CBAVD is an important cause of sterility in men and could represent an incomplete form of cystic fibrosis, as the majority of men suffering from cystic fibrosis lack the vas deferens.
      SIMILARITY: Belongs to the ABC transporter family. CFTR transporter (TC 3.A.1.202) subfamily. & Contains 2 ABC transmembrane type-1 domains. & Contains 2 ABC transporter domains.
      Molecular Weight170 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot on human T84 colon carcinoma epithelial RIPA cell lysates or CFTR-transfected BHK.

      Western Blot Analysis:
      0.5-2 μg/mL of this lot detected CFTR in human T84 colon carcinoma epithelial RIPA cell lysates. A previous lot detected CFTR in CFTR-transfected BHK.

      Note: CFTR can run as a diffuse protein on SDS-PAGE.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-CFTR, clone MM13-4 - 2344663 2344663
      Anti-CFTR, clone MM13-4 - 1953325 1953325
      Anti-CFTR, clone MM13-4 - 1993901 1993901
      Anti-CFTR, clone MM13-4 - 20147 20147
      Anti-CFTR, clone MM13-4 - 2193147 2193147
      Anti-CFTR, clone MM13-4 - 2207249 2207249
      Anti-CFTR, clone MM13-4 - 25374 25374
      Anti-CFTR, clone MM13-4 - 32547 32547
      Anti-CFTR, clone MM13-4 - DAM1411767 DAM1411767
      Anti-CFTR, clone MM13-4 - DAM1501796 DAM1501796

      References

      Reference overviewPub Med ID
      Analysis of CFTR folding and degradation in transiently transfected cells.
      Diane E Grove,Meredith F N Rosser,Richard L Watkins,Douglas M Cyr
      Methods in molecular biology (Clifton, N.J.)  741  2011

      Show Abstract
      21594788 21594788
      Comparative biology of cystic fibrosis animal models.
      Fisher, JT; Zhang, Y; Engelhardt, JF
      Methods in molecular biology (Clifton, N.J.)  742  311-34  2011

      Show Abstract
      21547741 21547741
      Epigenetic memory at malaria virulence genes.
      Chookajorn, T; Dzikowski, R; Frank, M; Li, F; Jiwani, AZ; Hartl, DL; Deitsch, KW
      Proceedings of the National Academy of Sciences of the United States of America  104  899-902  2007

      Show Abstract Full Text Article
      17209011 17209011
      Characterization of polyclonal and monoclonal antibodies to cystic fibrosis transmembrane conductance regulator.
      Kartner, N and Riordan, J R
      Meth. Enzymol., 292: 629-52 (1998)  1998

      9711588 9711588

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies