06-1339 | Anti-CHD-1 Antibody

06-1339
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, ICCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number06-1339
      DescriptionAnti-CHD-1 Antibody
      Alternate Names
      • chromodomain helicase DNA binding protein 1
      • ATP-dependent helicase CHD1
      • chromodomain-helicase-DNA-binding protein 1
      Background InformationChromodomain-helicase-DNA-binding protein 1 (CHD-1) is a member of the SNF2/RAD54 helicase family and is comprised of two chromo domains, one helicase ATP-binding domain, and one helicase C-terminal domain. CHD-1 is a regulator for RNA polymerase I transcription, polymerase II transcription, and a negative regulator for DNA replication. It is a chromatin-remodeling factor dependent on ATP and acting as a component for substrate recognition in HAT complex SAGA transcription. Studies have demonstrated CHD-1 involvement in HDAC activity and in the regulation of pre-mRNA splicing efficacy. CHD-1 is a required component in the maintenance of embryonic stem (ES) cell pluripotency.
      References
      Product Information
      FormatAffinity Purified
      Control
      • HeLa nuclear extract
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-CHD-1 Antibody is a Rabbit Polyclonal Antibody for detection of CHD-1 also known as chromodomain helicase DNA binding protein 1, ATP-dependent helicase CHD1 & has been validated in WB, ICC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a previous lot detected CHD-1 in NIH/3T3 and HeLa cells.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to human CHD-1.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteDemonstrated to react with human and mouse.
      Other homologies: Rat (91% sequence homology). Chimpanzee (99% sequence homology).
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe CHD family of proteins is characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD genes alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template. [provided by RefSeq].
      Gene Symbol
      • CHD1
      • CHD-1
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: ATP-dependent chromatin-remodeling factor which functions as substrate recognition component of the transcription regulatory histone acetylation (HAT) complex SAGA. Regulates polymerase II transcription. Also required for efficient transcription by RNA polymerase I, and more specifically the polymerase I transcription termination step. Regulates negatively DNA replication. Not only involved in transcription-related chromatin-remodeling, but also required to maintain a specific chromatin configuration across the genome. Is also associated with histone deacetylase (HDAC) activity (By similarity). Required for the bridging of SNF2, the FACT complex, the PAF complex as well as the U2 snRNP complex to H3K4me3. Functions to modulate the efficiency of pre-mRNA splicing in part through physical bridging of spliceosomal components to H3K4me3. Required for maintaining open chromatin and pluripotency in embryonic stem cells. Ref.10

      CATALYTIC ACTIVITY: ATP + H2O = ADP + phosphate.

      SUBUNIT STRUCTURE: Component of the SAGA complex (By similarity). Interacts with BCLAF1, NCoR, SRP20 and SAFB By similarity. Specifically interacts with methylated H3K4me2 and H3K4me3. Interacts with the FACT complex, the PAF complex and the U2 snRNP. Interacts directly with PAF1, SFA3A1, SFA3A2, SFA3A3, SNF2 and SSRP1.

      SUBCELLULAR LOCATION: Nucleus. Cytoplasm (By similarity). Note: Is released into the cytoplasm when cells enter mitosis and is reincorporated into chromatin during telophase-cytokinesis (By similarity).

      DOMAIN: The 2 chromodomains are involved in the binding to the histone H3 methyllysine at position 4 (H3K4me3). Ref.8

      SEQUENCE SIMILARITIES: Belongs to the SNF2/RAD54 helicase family.

      Contains 2 chromo domains.

      Contains 1 helicase ATP-binding domain.

      Contains 1 helicase C-terminal domain.
      Molecular Weight~170 kDa observed. Calculated MW ~197 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa nuclear extract.

      Western Blot Analysis: 0.25 µg/mL of this antibody detected CHD-1 in 10 µg of HeLa nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies