04-218 | Anti-CREB Antibody, clone E306, rabbit monoclonal

100 µL  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RFC, ICC, IF, IH(P), IP, WBRbCulture SupernatantMonoclonal Antibody
      Catalogue Number04-218
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-CREB Antibody, clone E306, rabbit monoclonal
      Alternate Names
      • cAMP responsive element binding protein
      • active transcription factor CREB
      • transactivator protein
      Background InformationCREB, cyclic AMP (cAMP) response element-binding protein, is a ubiquitously expressed basic leucine zipper transcription factor that functions in glucose homeostasis and stimulates the expression of numerous genes in response to growth factors, hormones, neurotransmitters, ion fluxes, stress signals, and has been implicated in memory because if its apparent role in synaptic plasticity. CREB functions either by itself or with related family members, which include CREB, CREM, and ATF-1, by forming homo- and heterodimers through their basic leucine zipper (bZIP) domains. The CREB signaling pathway is activated by extracellular ligands that bind to cell surface receptors and relay their message through various intracellular second messengers resulting ultimately in the activation of nuclear CREB through phosphorylation of the transactivation domain at serine 1332,4. The classical model of CREB activation is through PKA (cAMP-dependent protein kinase). In the non-active state, PKA resides in the cytoplasm as an inactive heterotetramer of paired regulatory and catalytic subunits. Stimulation of cAMP causes the releases of the catalytic subunits of PKA that passively diffuse into the nucleus and initiates gene expression by phosphorylating CREB at its transactivation domain on serine 1331,2,3,4. CREB mediates the activation of cAMP-responsive genes by binding as a dimer to a conserved cAMP-responsive element (CRE), 5’-TGACGTCA-3’, located within the promoter region of target genes. Target gene activation is terminated by the serine/threonine phosphatases PP-1-mediated dephospho-rylation of CREB.
      Product Information
      FormatCulture Supernatant
      • A431 whole cell lysate.
      Presentation100 μL of rabbit monoclonal IgG in 60% storage buffer (50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% sodium azide and 0.05% BSA) and 40% glycerol.
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-CREB Antibody, clone E306, rabbit detects level of CREB & has been published & validated for use in FC, IC, IF, IH(P), IP & WB.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      • Immunofluorescence
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      Application NotesFlow Cytometry: 1:40 dilution from a previous lot was used on flow cytometry.

      Immunohistochemistry: 1:250-500 dilution from a previous lot was used on immunohistochemistry.

      Immunoprecipitation: 1:80 dilution from a previous lot was used on immunoprecipitation.

      Immunocytochemistry: 1:250-500 dilution from a previous lot was used on immunocytochemistry.

      Biological Information
      ImmunogenKLH conjugated to CREB linear peptide.
      SpecificityThis antibody recognizes CREB.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteProven to react with human, rat and mouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MGC9284
      • CREB
      UniProt Number
      UniProt SummaryFunction: This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transciption activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity.
      Subunit structure: Interacts with PPRC1. Binds DNA as a dimer. This dimer is stabilized by magnesium ions. Interacts, through the bZIP domain, with the coactivators TORC1/CRTC1, TORC2/CRTC2 and TORC3/CRTC3. When phosphorylated on Ser-133, binds CREBBP By similarity.
      Subcellular location: Nucleus.
      Post-translational modification: Stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding By similarity. Phosphorylated upon DNA damage, probably by ATM or ATR.
      Sequence similarities: Belongs to the bZIP family.
      Contains 1 bZIP domain.
      Contains 1 KID (kinase-inducible) domain.
      Molecular Weight~43 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on A431 whole cell lysate.

      Western Blot Analysis:
      A 1:1000 dilution of this lot was used to detect CREB in A431 whole cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-CREB, clone E306 2483192
      Anti-CREB, clone E306 - 2118907 2118907
      Anti-CREB, clone E306 - 2139981 2139981
      Anti-CREB, clone E306 - 2328300 2328300
      Anti-CREB, clone E306 - 2383459 2383459
      Anti-CREB, clone E306 - 2428505 2428505
      Anti-CREB, clone E306 - NG1571166 NG1571166
      Anti-CREB, clone E306 - NG1726924 NG1726924
      Anti-CREB, clone E306 - NG1800777 NG1800777
      Anti-CREB, clone E306 - NG1843042 NG1843042


      Reference overviewApplicationPub Med ID
      CB1 receptor-mediated signaling underlies the hippocampal synaptic, learning, and memory deficits following treatment with JWH-081, a new component of spice/K2 preparations.
      Basavarajappa, BS; Subbanna, S
      Hippocampus  24  178-88  2014

      Show Abstract
      Western Blotting24123667 24123667

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