05-621 | Anti-CUGBP1 Antibody, clone 3B1

05-621
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, H, M, Po, R, RbEMSA, IP, WB, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-621
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-CUGBP1 Antibody, clone 3B1
      Background InformationMyotonic dystrophy (MD) is an autosomal dominant neuromuscular disease that is associated with a (CTG)n repeat expansion in the 3'-untranslated region of the myotonin protein kinase (Mt-PK) gene. A (CUG)n oligonucleotides triplet repeat pre-mRNA/mRNA binding protein may play an important role in DM pathogenesis. HeLa cell protein, CUG-BP1, has been purified based upon its ability to bind specifically to (CUG)8 oligonucleotides in vitro. CUG-BP1 is the major (CUG)8 binding activity in normal cells. CUG-BP1 has been identified as isoforms of a novel heterogeneous nuclear ribonucleoprotein (hnRNP), hNab50. The CUG-BP/hNab50 protein is localized predominantly in the nucleus and is associated with polyadenylated RNAs in vivo. In vitro RNA-binding/photocrosslinking studies demonstrate that CUG-BP/hNab50 binds to RNAs containing the Mt-PK 3'-UTR.
      References
      Product Information
      FormatPurified
      Control
      • HeLa lysates
      Presentation0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
      Applications
      ApplicationAnti-CUGBP1 Antibody, clone 3B1 is a high quality Mouse Monoclonal Antibody for the detection of CUGBP1 & has been validated in EMSA, IP, WB, ICC.
      Key Applications
      • Electrophoretic Mobility Shift Assay
      • Immunoprecipitation
      • Western Blotting
      • Immunocytochemistry
      Biological Information
      ImmunogenFull-length GST fusion protein corresponding to human CUG-BP1, also known as heterogeneous nuclear ribonucleoprotein (hnRNP) hNab50
      Cloneclone 3B1
      HostMouse
      SpecificityCUG-BP1
      IsotypeIgG
      Species Reactivity
      • Bovine
      • Human
      • Mouse
      • Pig
      • Rat
      • Rabbit
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryMembers of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. This gene may play a role in myotonic dystrophy type 1 (DM1) via interactions with the dystrophia myotonica-protein kinase (DMPK) gene. Alternative splicing results in multiple transcript variants encoding different isoforms.
      Gene Symbol
      • CUGBP1
      • BRUNOL2
      • CELF-1
      • CUGBP
      • NAB50
      • CELF1
      • CUG-BP
      • hNab50
      • CUG-BP1
      Purification MethodProtein G Chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q92879 # RNA-binding protein implicated in the regulation of several post-transcriptional events. Involved in pre-mRNA alternative splicing, mRNA translation and stability. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Acts as both an activator and repressor of a pair of coregulated exons: promotes inclusion of the smooth muscle (SM) exon but exclusion of the non-muscle (NM) exon in actinin pre- mRNAs. Activates SM exon 5 inclusion by antagonizing the repressive effect of PTB. Promotes exclusion of exon 11 of the INSR pre-mRNA. Increases translation and controls the choice of translation initiation codon of CEBPB mRNA. Increases mRNA translation of CEBPB in aging liver (By similarity). Increases translation of CDKN1A mRNA by antagonizing the repressive effect of CALR3. Mediates rapid cytoplasmic mRNA deadenylation. Recruits the deadenylase PARN to the poly(A) tail of EDEN-containing mRNAs to promote their deadenylation. Required for completion of spermatogenesis (By similarity). Binds to (CUG)n triplet repeats in the 3'-UTR of transcripts such as DMPK and to Bruno response elements (BREs). Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA. Binds to AU-rich sequences (AREs or EDEN-like) localized in the 3'-UTR of JUN and FOS mRNAs. Binds to the 5'-region of CDKN1A and CEBPB mRNAs. Binds with the 5'-region of CEBPB mRNA in aging liver (By similarity).


      SIZE: 486 amino acids; 52063 Da

      SUBUNIT: Component of an EIF2 complex at least composed of CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Associates with polysomes (By similarity). Interacts with PARN.

      SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=RNA-binding activity is detected in both nuclear and cytoplasmic compartments.
      TISSUE SPECIFICITY: Ubiquitous.



      PTM: Phosphorylated. Its phosphorylation status increases in senescent cells.
      DISEASE:SwissProt: Q92879 # CUGBP1 may be involved in the mechanism of myotonic distrophy. It binds to the CUG repeat expansion of the CC untranslated region of the myotonin protein kinase (Mt-PK) gene.

      SIMILARITY: SwissProt: Q92879 ## Belongs to the CELF/BRUNOL family. & Contains 3 RRM (RNA recognition motif) domains.
      Molecular Weight50kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on HeLa nuclear extract
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      05-621 - 2293828 2293828
      Anti-CUG-BP1, clone 3B1 (mouse monoclonal IgG) 2890795
      Anti-CUG-BP1, clone 3B1 (mouse monoclonal IgG) - 2106956 2106956
      Anti-CUG-BP1, clone 3B1 (mouse monoclonal IgG) - DAM1791303 DAM1791303
      Anti-CUG-BP1, clone 3B1 - 20448 20448
      Anti-CUG-BP1, clone 3B1 - 2178416 2178416
      Anti-CUG-BP1, clone 3B1 - 33587 33587
      Anti-CUG-BP1, clone 3B1 - DAM1574323 DAM1574323
      Anti-CUG-BP1, clone 3B1 - DAM1644547 DAM1644547
      Anti-CUG-BP1, clone 3B1 - JBC1381953 JBC1381953

      References

      Reference overviewApplicationPub Med ID
      Profiling post-transcriptionally networked mRNA subsets using RIP-Chip and RIP-Seq.
      Jayaseelan, S; Doyle, F; Tenenbaum, SA
      Methods (San Diego, Calif.)  67  13-9  2014

      Show Abstract
      24257445 24257445
      HTS-Compatible Patient-Derived Cell-Based Assay to Identify Small Molecule Modulators of Aberrant Splicing in Myotonic Dystrophy Type 1.
      O'Leary, DA; Vargas, L; Sharif, O; Garcia, ME; Sigal, YJ; Chow, SK; Schmedt, C; Caldwell, JS; Brinker, A; Engels, IH
      Current chemical genomics  4  9-18  2010

      Show Abstract Full Text Article
      20502647 20502647
      Reversible model of RNA toxicity and cardiac conduction defects in myotonic dystrophy.
      Mahadevan, MS; Yadava, RS; Yu, Q; Balijepalli, S; Frenzel-McCardell, CD; Bourne, TD; Phillips, LH
      Nature genetics  38  1066-70  2006

      Show Abstract
      Western Blotting16878132 16878132
      Identification of a (CUG)n triplet repeat RNA-binding protein and its expression in myotonic dystrophy.
      Timchenko, L T, et al.
      Nucleic Acids Res., 24: 4407-14 (1996)  1996

      Show Abstract
      8948631 8948631

      Brochure

      Title
      RNA-Binding Protein Immunoprecipitation

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies