07-1496 | Anti-CaM Kinase II Antibody

07-1496
100 µL   
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      Overview

      Replacement Information
      Replacement Information07-1496 is a recommended replacement for AB3111.

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, M, B, Ht, Gp, Ch, RbWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-1496
      ReplacesAB3111
      DescriptionAnti-CaM Kinase II Antibody
      Background InformationThe Ca2+/Calmodulin-Dependent Kinase family includes CaM Kinases I through IV, Phosphorylase Kinase, and MLCK. The CaM Kinases are inactive in the absence of stimuli; binding to Ca2+/Calmodulin induces conformational changes and activation. CaM Kinases have broad substrate specificity, with many cellular functions.
      References
      Product Information
      FormatAffinity Purified
      Control
      • HEK293 cell lysate
      PresentationPurified rabbit polyclonal IgG in buffer containing borate buffered saline and phosphate buffered saline containing 0.05% sodium azide.
      Applications
      ApplicationAnti-CaM Kinase II Antibody is an antibody against CaM Kinase II for use in WB.
      Key Applications
      • Western Blotting
      Application NotesOptimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenSynthetic peptide corresponding to the N-terminus of Rat CaMK II
      EpitopeN-terminus
      HostRabbit
      SpecificityRecognizes proteins of 50-72 kDa, corresponding to apparent molecular mass of CaMKII isoforms on SDS-PAGE immunoblots. Recombinant rat calmodulin-dependent protein kinase II α subunits expressed in sf9 insect cells are also detected. As the sequences of the rat α, δ, and γ isoforms are conserved over this amino terminus region, this antibody is expected to recognize the δ, γ, and α isoforms. Antibody is not expected to cross-react with the β isoform. Proteins of unknown identity may be detected on immunoblot analysis with some lysates (40, 45 and 90 kDa).
      IsotypeIgG
      Species Reactivity
      • Human
      • Rat
      • Mouse
      • Bovine
      • Hamster
      • Guinea Pig
      • Chicken
      • Rabbit
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe product of this gene belongs to the Serine/Threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This enzyme is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Two transcript variants encoding distinct isoforms have been identified for this gene
      Gene Symbol
      • CAMK2A
      • KIAA0968
      • CAMKA
      • EC 2.7.11.17
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q9UQM7 # CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity (By similarity).
      SIZE: 478 amino acids; 54030 Da
      SUBUNIT: CAMK2 is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. Interacts with BAALC, MPDZ, SYN1 and SYNGAP1 (By similarity).
      SUBCELLULAR LOCATION: Cell junction, synapse, presynaptic cell membrane (By similarity). Cell junction, synapse (By similarity). Note=Postsynaptic lipid rafts (By similarity).
      SIMILARITY: SwissProt: Q9UQM7 ## Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. & Contains 1 protein kinase domain.
      Molecular Weight50-72 kDa, corresponding to apparent molecular mass of CaMKII isoforms.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on HEK293 lysates.

      Western Blot Analysis:
      1:500 to 1:1,000 dilution of this lot detected CaM Kinase II on 10 μg of HEK293 lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C in undiluted aliquots from date of receipt. For optimal storage, aliquot and store at -20°C or -70°C.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-CaM Kinase II 2475659
      Anti-CaM Kinase II 3075613
      Anti-CaM Kinase II - 2370125 2370125
      Anti-CaM Kinase II - 2041600 2041600
      Anti-CaM Kinase II - 2199714 2199714
      Anti-CaM Kinase II - 2274277 2274277
      Anti-CaM Kinase II - 2884648 2884648
      Anti-CaM Kinase II - DAM1570841 DAM1570841
      Anti-CaM Kinase II - JH1774728 JH1774728
      Anti-CaM Kinase II - NG1636569 NG1636569

      References

      Reference overviewPub Med ID
      Suppression of eIF2α kinases alleviates Alzheimer's disease-related plasticity and memory deficits.
      Ma, T; Trinh, MA; Wexler, AJ; Bourbon, C; Gatti, E; Pierre, P; Cavener, DR; Klann, E
      Nature neuroscience  16  1299-305  2013

      Show Abstract
      23933749 23933749
      CaMKII regulates the density of central glutamatergic synapses in vivo.
      Rongo, C and Kaplan, J M
      Nature, 402: 195-9 (1999)  1999

      10647013 10647013
      Identification and expression of delta-isoforms of the multifunctional Ca2+/calmodulin-dependent protein kinase in failing and nonfailing human myocardium.
      Hoch, B, et al.
      Circ. Res., 84: 713-21 (1999)  1999

      Show Abstract
      10189359 10189359
      Inhibition of calcium/calmodulin-dependent protein kinase II in rat hippocampus attenuates morphine tolerance and dependence.
      Fan, G H, et al.
      Mol. Pharmacol., 56: 39-45 (1999)  1999

      Show Abstract
      10385682 10385682
      Regulation of DLG localization at synapses by CaMKII-dependent phosphorylation.
      Koh, Y H, et al.
      Cell, 98: 353-63 (1999)  1999

      Show Abstract
      10458610 10458610
      AlphaCaMKII binding to the C-terminal tail of NMDA receptor subunit NR2A and its modulation by autophosphorylation.
      Gardoni, F, et al.
      FEBS Lett., 456: 394-8 (1999)  1999

      10462051 10462051
      Downregulation of delta CaM kinase II in human tumor cells.
      Tombes, R M, et al.
      Biochim. Biophys. Acta, 1452: 1-11 (1999)  1999

      Show Abstract
      10525155 10525155
      Regional distribution of type II Ca2+/calmodulin-dependent protein kinase in rat brain.
      Erondu, N E and Kennedy, M B
      J. Neurosci., 5: 3270-7 (1985)  1985

      Show Abstract
      4078628 4078628

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies