04-1081 | Anti-Calcium/Calmodulin Kinase IV Antibody, clone EP2564Y, rabbit monoclonal

04-1081
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, ICCRbUnpurifiedMonoclonal Antibody
      Description
      Catalogue Number04-1081
      DescriptionAnti-Calcium/Calmodulin Kinase IV Antibody, clone EP2564Y, rabbit monoclonal
      Alternate Names
      • CAM kinase IV
      • CAM kinase- GR
      • CAM kinase-GR
      • CaMK IV
      • brain Ca(2+)-calmodulin-dependent protein kinase type IV
      • brain Ca++-calmodulin-dependent protein kinase type IV
      • calcium/calmodulin-dependent protein kinase IV
      • calcium/calmodulin-dependent protein kinase type IV
        catalytic chain
      Background InformationThe Ca2+/Calmodulin-Dependent Kinase family includes CaM Kinases I through IV, Phosphorylase Kinase, and MLCK. The CaM Kinases are inactive in the absence of stimuli; binding to Ca2+/Calmodulin induces conformational changes and activation. CaM Kinases have broad substrate specificity, with many cellular functions.
      References
      Product Information
      FormatUnpurified
      Control
      • Jurkat and Human brain lysates
      PresentationRabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
      Applications
      ApplicationAnti-Calcium/Calmodulin Kinase IV Antibody, clone EP2564Y, Rabbit is an antibody against Calcium/Calmodulin Kinase IV for use in WB & IC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:100-250 dilution from a previous lot was used in IC.
      Biological Information
      ImmunogenSynthetic peptide corresponding to residues near the N-terminus of human Calcium/Calmodulin Kinase IV.
      EpitopeN-terminus
      CloneEP2564Y
      HostRabbit
      SpecificityThis antibody recognizes Calcium/Calmodulin Kinase IV.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe product of this gene belongs to the serine/threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This enzyme is a multifunctional serine/threonine protein kinase with limited tissue distribution, that has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells
      Gene Symbol
      • CaMK-GR
      • EC 2.7.11.17
      • MGC36771
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. May be involved in transcriptional regulation. May be involved in regulation of microtubule dynamics. In vitro, phosphorylates CREB1, CREBBP, PRM2, MEF2A, MEF2D and STMN1/OP18. May be involved in spermatogenesis. May play a role in the consolidation/retention of hippocampus-dependent long-term memory By similarity.
      CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
      ENZYME REGULATION:Activated by Ca2+/calmodulin. Binding of calmodulin may releave intrasteric autoinhibition. Must be phosphorylated to be maximally active. Phosphorylated by CAMKK1 or CAMKK2. Autophosphorylation of the N-terminus is required for full activation. In part, activity is independent on Ca2+/calmodulin and autophosphorylation of Ser-336 allows to switch to a Ca2+/calmodulin-independent state By similarity. Probably inactivated by serine/threonine protein phosphatase 2A.

      SUBUNIT STRUCTURE: Monomer By similarity. Interacts with serine/threonine protein phosphatase 2A catalytic subunit, PPP2CA or PPP2CB. The interaction with PP2CA or PP2CB is mutually exclusive with binding to Ca2+/calmodulin.

      SUBCELLULAR LOCATION: Cytoplasm By similarity. Nucleus By similarity. Note: Substantial localization in certain neuronal nuclei. In spermatids associated with chromatin and nuclear matrix By similarity.
      TISSUE SPECIFICTY: Expressed in epithelial ovarian cancer tissue. Ref.8
      PTM: Autophosphorylated and phosphorylated by CAMKK1 and CAMKK2 By similarity. Dephosphorylated by serine/threonine protein phosphatase 2A, probably on Thr-200.
      SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.Contains 1 protein kinase domain.

      Molecular Weight~60 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot on Jurkat and Human brain lysates.
      Western Blot Analysis: A 1:1,000-2,000 dilution of this antibody was used to detect Calcium/Calmodulin Kinase IV in Jurkat and Human brain lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Calcium/Calmodulin Kinase IV, clone EP2564Y, Rabbit Monoclonal - 2365799 2365799
      Anti-Calcium/Calmodulin Kinase IV, clone EP2564Y, Rabbit Monoclonal - 2424084 2424084
      Anti-Calcium/Calmodulin Kinase IV, clone EP2564Y, Rabbit Monoclonal - 2060501 2060501
      Anti-Calcium/Calmodulin Kinase IV, clone EP2564Y, Rabbit Monoclonal - 2203102 2203102
      Anti-Calcium/Calmodulin Kinase IV, clone EP2564Y, Rabbit Monoclonal - NRG1649495 NRG1649495

      References

      Reference overviewPub Med ID
      Subgroup-elimination transcriptomics identifies signaling proteins that define subclasses of TRPV1-positive neurons and a novel paracrine circuit.
      Isensee, J; Wenzel, C; Buschow, R; Weissmann, R; Kuss, AW; Hucho, T
      PloS one  9  e115731  2014

      Show Abstract
      25551770 25551770

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies