07-482 | Anti-Calmodulin Binding Protein Epitope Tag Antibody

07-482
200 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      E. coliWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-482
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Calmodulin Binding Protein Epitope Tag Antibody
      Background InformationCalmodulin (CaM), a small 17 kDa ubiquitous Ca2+-binding protein acts as an intracellular Ca2+ receptor and transduces Ca2+ transients, usually in response to growth factors. It mediates the activity of a number of Ca2+ regulating enzymes, including protein kinases, phosphatases, nitric oxide synthases, and phosphodiesterase.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Lysates from transformed E. coli.
      PresentationPurified rabbit IgG in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH7.4.
      Applications
      ApplicationAnti-Calmodulin Binding Protein Epitope Tag Antibody detects level of Calmodulin Binding Protein Epitope Tag & has been published & validated for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide (MKRRWKKNFIAVSAANRFKKISSSGAL) corresponding to the Calmodulin Binding Protein (CBP) epitope tag found in several E. coli expression vectors. The immunizing sequence is identical in rabbit, mouse, and rat.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes recombinant proteins containing the calmodulin binding protein epitope tag.
      IsotypeIgG
      Species Reactivity
      • E. coli
      Antibody TypePolyclonal Antibody
      Purification MethodAffinity Purfied
      Molecular WeightThe molecular weight is related to the tagged protein.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot in lysates from transformed E. coli.

      Western Blot Analysis:
      A 1:2000-1:10000 dilution of this lot detected a recombinant protein containing the calmodulin binding protein epitope tag in lysates from transformed E. coli.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Calmodulin Binding Protein -2787702 2787702
      Anti-Calmodulin Binding Protein Epitope Tag - 2167789 2167789
      Anti-Calmodulin Binding Protein Epitope Tag - 2424770 2424770
      Anti-Calmodulin Binding Protein Epitope Tag - 2465198 2465198
      Anti-Calmodulin Binding Protein Epitope Tag - 0701049682 0701049682
      Anti-Calmodulin Binding Protein Epitope Tag - 2044809 2044809
      Anti-Calmodulin Binding Protein Epitope Tag - 2073138 2073138
      Anti-Calmodulin Binding Protein Epitope Tag - 2116605 2116605
      Anti-Calmodulin Binding Protein Epitope Tag - 2201577 2201577
      Anti-Calmodulin Binding Protein Epitope Tag - 2225246 2225246

      References

      Reference overviewApplicationPub Med ID
      Dynactin integrity depends upon direct binding of dynamitin to Arp1.
      Cheong, FK; Feng, L; Sarkeshik, A; Yates, JR; Schroer, TA
      Molecular biology of the cell  25  2171-80  2014

      Show Abstract
      24829381 24829381
      Activation of the Nlrp1b inflammasome by reduction of cytosolic ATP.
      Liao, KC; Mogridge, J
      Infection and immunity  81  570-9  2013

      Show Abstract
      Western Blotting23230290 23230290
      Proteolytic processing of Nlrp1b is required for inflammasome activity.
      Frew, BC; Joag, VR; Mogridge, J
      PLoS pathogens  8  e1002659  2012

      Show Abstract
      22536155 22536155
      Role of km23-1 in RhoA/actin-based cell migration.
      Jin, Q; Pulipati, NR; Zhou, W; Staub, CM; Liotta, LA; Mulder, KM
      Biochemical and biophysical research communications  428  333-8  2012

      Show Abstract
      23079622 23079622
      Development of the "Three-step MACS": a novel strategy for isolating rare cell populations in the absence of known cell surface markers from complex animal tissue.
      Lee, MY; Lufkin, T
      Journal of biomolecular techniques : JBT  23  69-77  2012

      Show Abstract
      Flow Cytometry22951961 22951961
      SWI/SNF and Asf1 independently promote derepression of the DNA damage response genes under conditions of replication stress.
      Minard, LV; Lin, LJ; Schultz, MC
      PloS one  6  e21633  2011

      Show Abstract
      21738741 21738741
      Asf1 can promote trimethylation of H3 K36 by Set2.
      Lin, LJ; Minard, LV; Johnston, GC; Singer, RA; Schultz, MC
      Molecular and cellular biology  30  1116-29  2010

      Show Abstract Full Text Article
      20048053 20048053
      Mutational analysis of the C-terminal FATC domain of Saccharomyces cerevisiae Tra1.
      Hoke, SM; Irina Mutiu, A; Genereaux, J; Kvas, S; Buck, M; Yu, M; Gloor, GB; Brandl, CJ
      Current genetics  56  447-65  2010

      Show Abstract Full Text Article
      Western Blotting20635087 20635087
      A glycolytic burst drives glucose induction of global histone acetylation by picNuA4 and SAGA.
      Friis, RM; Wu, BP; Reinke, SN; Hockman, DJ; Sykes, BD; Schultz, MC
      Nucleic acids research  37  3969-80  2009

      Show Abstract Full Text Article
      19406923 19406923
      Expression of Nlrp1b inflammasome components in human fibroblasts confers susceptibility to anthrax lethal toxin.
      Liao, KC; Mogridge, J
      Infection and immunity  77  4455-62  2009

      Show Abstract
      Western Blotting19651869 19651869