04-571 | Anti-Caspase 2 Antibody, rabbit monoclonal

04-571
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MFC, WBRbPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-571
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Caspase 2 Antibody, rabbit monoclonal
      Background InformationCaspase-2 (Nedd2/ICH-1) is part of a family of aspartate-specific cysteine proteases that participate in programmed cell death, or apoptosis. Caspase-2 is localized to the nucleus, and has been shown to play roles in both positive and negative regulation of apoptosis (1). Upon apoptotic stimulation, pro-caspase-9 is cleaved in a series of steps to produce an 18-kDa large subunit and a 12-kDa small fragment (2). Caspase-2 can be a nuclear initiator caspase, as it has been shown to precede the processing and activation of caspase-8, -9, and -3 (3). Caspase-2 might also act as an effector caspase; in apoptotic pathways resulting from UV-induced DNA damage, processing of caspase-2 occurs downstream of caspase-9 and caspase-3 activation (4).
      References
      Product Information
      FormatPurified
      PresentationBuffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
      Applications
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect Caspase 2 using this Anti-Caspase 2 Antibody validated for use in FC & WB.
      Key Applications
      • Flow Cytometry
      • Western Blotting
      Application NotesWB: 1:500
      Flow Cytometry: 1:10
      Biological Information
      ImmunogenA synthetic peptide corresponding to N-terminus of human Caspase-2 p15 subunit. The antibody should recognize both pro-form (~50kDa) and p15 cleaved-form of Caspase-2.
      CloneY61
      HostRabbit
      SpecificityRecognizes Human, Mouse Caspase-2.
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse (positive control: Jurkat cell lysate)
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The proteolytic cleavage of this protein is induced by a variety of apoptotic stimuli. Alternative splicing of this gene results in multiple transcript variants that encode different isoforms.
      Gene Symbol
      • CASP2
      • NEDD2
      • ICH1
      • ICH-1L/1S
      • ICH-1L
      • NEDD-2
      • CASP-2
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P42575 # Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival.
      SIZE: 452 amino acids; 50685 Da
      SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a p18 subunit and a p12 subunit. Interacts with LRDD.
      TISSUE SPECIFICITY: Expressed in larger amounts in the embryonic lung, liver and kidney than in the heart and brain. In the adults higher level expression is seen in the placenta, lung, kidney, pancreas than in the heart, brain, liver and skeletal muscle.
      PTM: The mature protease can process its own propeptide, but not that of other caspases.
      SIMILARITY: SwissProt: P42575 ## Belongs to the peptidase C14 family. & Contains 1 CARD domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -20 °C. Stable for 12 months from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Caspase-2, clone Y61 (rabbit monoclonal) - 2433568 2433568
      Anti-Caspase-2, clone Y61 - 2010076 2010076
      Anti-Caspase-2, clone Y61 - 2054798 2054798
      Anti-Caspase-2, clone Y61 - DAM1421471 DAM1421471
      Anti-Caspase-2, clone Y61 - NG1641357 NG1641357
      Anti-Caspase-2, clone Y61 - NG1696112 NG1696112
      Anti-Caspase-2, clone Y61 - NG1914813 NG1914813

      References

      Reference overviewPub Med ID
      Apoptosis signaling triggered by the marine alkaloid ascididemin is routed via caspase-2 and JNK to mitochondria
      Dirsch, Verena M, et al
      Oncogene, 23:1586-93 (2004)  2004

      14716300 14716300
      Caspase-2-induced apoptosis is dependent on caspase-9, but its processing during UV- or tumor necrosis factor-dependent cell death requires caspase-3
      Paroni, G, et al
      J Biol Chem, 276:21907-15 (2001)  2001

      11399776 11399776
      Activation of caspase-2 in apoptosis
      Li, H, et al
      J Biol Chem, 272:21010-7 (1997)  1997

      9261102 9261102
      Ich-1, an Ice/ced-3-related gene, encodes both positive and negative regulators of programmed cell death
      Wang, L, et al
      Cell, 78:739-50 (1994)  1994

      8087842 8087842

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      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies