05-654 | Anti-Caspase 3 Antibody, clone 4-1-18

05-654
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-654
      Replaces04-440; 04-1088
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Caspase 3 Antibody, clone 4-1-18
      References
      Product Information
      FormatPurified
      Presentation0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
      Applications
      ApplicationAnti-Caspase 3 Antibody, clone 4-1-18 is an antibody against Caspase 3 for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenN-terminal HIS tagged fusion protein corresponding to full length human Caspase 3
      Clone4-1-18
      HostMouse
      Specificityfull-length Caspase 3 and its subunit
      IsotypeIgG2a
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.
      Gene Symbol
      • CASP3
      • apopain
      • CASP-3
      • procaspase3
      • CPP32B
      • SCA-1
      • CPP-32
      • Apopain
      • CPP32
      • Yama
      Purification MethodProtein G Chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P42574 # Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp- -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.
      SIZE: 277 amino acids; 31608 Da
      SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit.
      SUBCELLULAR LOCATION: Cytoplasm.
      TISSUE SPECIFICITY: Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
      PTM: Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
      SIMILARITY: SwissProt: P42574 ## Belongs to the peptidase C14 family.
      Molecular Weight32 kDa (full-length), 17 kDa (subunit)
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from Jurkat cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Caspase 3, clone 4-1-18 (mouse monoclonal IgG2a) - 2343558 2343558
      Anti-Caspase 3, clone 4-1-18 - 22206 22206
      Anti-Caspase 3, clone 4-1-18 - 2531918 2531918

      References

      Reference overviewPub Med ID
      Caspases disrupt the nuclear-cytoplasmic barrier.
      Faleiro, L and Lazebnik, Y
      J. Cell Biol., 151: 951-9 (2000)  2000

      Show Abstract
      11085998 11085998
      Oncogene-dependent apoptosis is mediated by caspase-9.
      Fearnhead, H O, et al.
      Proc. Natl. Acad. Sci. U.S.A., 95: 13664-9 (1998)  1998

      Show Abstract
      9811857 9811857

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      Included Positive Control

      Catalogue Number Description  
      12-303 Jurkat Cell Lysate Show Pricing & Availability

      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies