06-775 | Anti-Caspase 8 Antibody

06-775
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number06-775
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Caspase 8 Antibody
      References
      Product Information
      FormatPurified
      Presentation0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
      Applications
      ApplicationAnti-Caspase 8 Antibody is an antibody against Caspase 8 for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenGST-tagged fusion protein corresponding to human Caspase 8
      HostRabbit
      SpecificityCaspase 8a & 8b; does not detect the proteolytic fragment
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.
      Gene Symbol
      • CASP8
      • CASP-8
      • FLICE
      • CAP4
      • MGC78473
      • MACH
      • procaspase-8
      • MCH5
      • ALPS2B
      Purification MethodProtein A chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q14790 # Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death- inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp- -AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoforms 5, 6, 7 and 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.
      SIZE: 479 amino acids; 55391 Da
      SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (p18) and a 10 kDa (p10) subunit. Interacts with FADD, CFLAR and PEA15. Isoform 9 interacts at the endoplasmic reticulum with a complex containing BCAP31, BAP29, BCL2 and/or BCL2L1.
      SUBCELLULAR LOCATION: Cytoplasm.
      TISSUE SPECIFICITY: Isoforms 1, 5 and 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus, and liver. Barely detectable in brain, testis, and skeletal muscle.DOMAIN:SwissProt: Q14790 Isoform 9 contains a N-terminal extension that is required for interaction with the BCAP31 complex.
      PTM: Generation of the subunits requires association with the death-inducing signaling complex (DISC), whereas additional processing is likely due to the autocatalytic activity of the activated protease. GZMB and CASP10 can be involved in these processing events. & Phosphorylated upon DNA damage, probably by ATM or ATR.
      DISEASE: SwissProt: Q14790 # Defects in CASP8 are the cause of caspase-8 deficiency (CASP8D) [MIM:607271]. CASP8D is a disorder resembling autoimmune lymphoproliferative syndrome (ALPS). It is characterized by lymphadenopathy, splenomegaly, and defective CD95-induced apoptosis of peripheral blood lymphocytes (PBLs). It leads to defects in activation of T-lymphocytes, B-lymphocytes, and natural killer cells leading to immunodeficiency characterized by recurrent sinopulmonary and herpes simplex virus infections and poor responses to immunization.
      SIMILARITY: Belongs to the peptidase C14 family. & Contains 2 DED (death effector) domains.
      Molecular WeightCaspase 8a (55kDa) and Caspase 8b (54kDa)
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from Jurkat cells, A431, K562, HeLa, HL-60 or MDA cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Caspase 8 (rabbit polyclonal IgG) - 2117147 2117147
      Anti-Caspase 8 (rabbit polyclonal IgG) - 2127811 2127811
      Anti-Caspase 8 (rabbit polyclonal IgG) - 2161132 2161132
      Anti-Caspase 8 - 17051 17051
      Anti-Caspase 8 - 24058 24058
      Anti-Caspase 8 - 33509 33509
      Anti-Caspase 8 - JBC1852378 JBC1852378
      Anti-Caspase 8 -2578143 2578143
      Anti-Caspase 8 -2613173 2613173
      Anti-Caspase 8 -2686212 2686212

      References

      Reference overviewPub Med ID
      Arabidopsis BREVIPEDICELLUS interacts with the SWI2/SNF2 chromatin remodeling ATPase BRAHMA to regulate KNAT2 and KNAT6 expression in control of inflorescence architecture.
      Zhao, M; Yang, S; Chen, CY; Li, C; Shan, W; Lu, W; Cui, Y; Liu, X; Wu, K
      PLoS genetics  11  e1005125  2015

      Show Abstract
      25822547 25822547
      Resveratrol Inhibits Respiratory Syncytial Virus-Induced IL-6 Production, Decreases Viral Replication, and Downregulates TRIF Expression in Airway Epithelial Cells.
      Xiao-Hong Xie,Na Zang,Si-Min Li,Li-Jia Wang,Yu Deng,Yun He,Xi-Qiang Yang,En-Mei Liu
      Inflammation  35  2012

      Show Abstract
      22391746 22391746
      TWEAK induces apoptosis through a death-signaling complex comprising receptor-interacting protein 1 (RIP1), Fas-associated death domain (FADD), and caspase-8.
      Ikner, A; Ashkenazi, A
      The Journal of biological chemistry  286  21546-54  2011

      Show Abstract
      21525013 21525013
      Aranorosin and a novel derivative inhibit the anti-apoptotic functions regulated by Bcl-2.
      Takayuki Nakashima, Rieko Tanaka, Yoshinori Yamashita, Yutaka Kanda, Mitsunobu Hara, Takayuki Nakashima, Rieko Tanaka, Yoshinori Yamashita, Yutaka Kanda, Mitsunobu Hara
      Biochemical and biophysical research communications  377  1085-90  2008

      Show Abstract
      18977202 18977202
      I-FLICE, a novel inhibitor of tumor necrosis factor receptor-1- and CD-95-induced apoptosis.
      Hu, S, et al.
      J. Biol. Chem., 272: 17255-7 (1997)  1997

      Show Abstract
      9211860 9211860
      Inhibition of death receptor signals by cellular FLIP.
      Irmler, M, et al.
      Nature, 388: 190-5 (1997)  1997

      Show Abstract
      9217161 9217161
      FLICE is predominantly expressed as two functionally active isoforms, caspase-8/a and caspase-8/b.
      Scaffidi, C, et al.
      J. Biol. Chem., 272: 26953-8 (1997)  1997

      Show Abstract
      9341131 9341131
      FLICE induced apoptosis in a cell-free system. Cleavage of caspase zymogens.
      Muzio, M, et al.
      J. Biol. Chem., 272: 2952-6 (1997)  1997

      Show Abstract
      9006941 9006941
      FLICE, a novel FADD-homologous ICE/CED-3-like protease, is recruited to the CD95 (Fas/APO-1) death--inducing signaling complex
      Muzio, M, et al
      Cell, 85:817-27 (1996)  1996

      8681377 8681377

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      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies