04-573 | Anti-Caspase-8 Antibody, rabbit monoclonal

04-573
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RIP, WB, FCRbPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-573
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Caspase-8 Antibody, rabbit monoclonal
      Background InformationCaspase-8 (MACH/FLICE/Mch5) is a member of a family of cysteine-requiring aspartate proteases that participate in the intracellular signaling cascade leading to apoptosis, or programmed cell death (1-4). Caspase-8 is recruited to death-inducing signaling complex (DISC) CD95 (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) and is subsequently activated by proteolytic cleavage into two residues, p10 and p18 (1-4). Active caspase-8, the first in the caspase signaling cascade, signals downstream caspases to eventually induce apoptosis.
      References
      Product Information
      FormatPurified
      PresentationBuffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
      Applications
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-Caspase-8 Antibody detects level of Caspase-8 & has been published & validated for use in IP, WB, FC.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Flow Cytometry
      Application NotesWB: 1:500
      Flow Cytometry: 1:20
      IP: 1:40
      Biological Information
      ImmunogenA synthetic peptide corresponding to N-terminal residues of human Caspase-8 subunit p18. The antibody should recognize both pro-form (55kDa) and p18 cleaved-form of Caspase-8.
      CloneE7
      HostRabbit
      SpecificityRecognizes Human, Rat Caspase-8.
      Species Reactivity
      • Human
      • Rat
      Species Reactivity NoteHuman, Rat (positive control: Hela cell lysate)
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.
      Gene Symbol
      • CASP8
      • MCH5
      • MGC78473
      • CASP-8
      • MACH
      • ALPS2B
      • FLICE
      • procaspase-8
      • CAP4
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q14790 # Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death- inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp- -AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoforms 5, 6, 7 and 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.
      SIZE: 479 amino acids; 55391 Da
      SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (p18) and a 10 kDa (p10) subunit. Interacts with FADD, CFLAR and PEA15. Isoform 9 interacts at the endoplasmic reticulum with a complex containing BCAP31, BAP29, BCL2 and/or BCL2L1.
      SUBCELLULAR LOCATION: Cytoplasm.
      TISSUE SPECIFICITY: Isoforms 1, 5 and 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus, and liver. Barely detectable in brain, testis, and skeletal muscle.DOMAIN:SwissProt: Q14790 Isoform 9 contains a N-terminal extension that is required for interaction with the BCAP31 complex.
      PTM: Generation of the subunits requires association with the death-inducing signaling complex (DISC), whereas additional processing is likely due to the autocatalytic activity of the activated protease. GZMB and CASP10 can be involved in these processing events. & Phosphorylated upon DNA damage, probably by ATM or ATR.
      DISEASE: SwissProt: Q14790 # Defects in CASP8 are the cause of caspase-8 deficiency (CASP8D) [MIM:607271]. CASP8D is a disorder resembling autoimmune lymphoproliferative syndrome (ALPS). It is characterized by lymphadenopathy, splenomegaly, and defective CD95-induced apoptosis of peripheral blood lymphocytes (PBLs). It leads to defects in activation of T-lymphocytes, B-lymphocytes, and natural killer cells leading to immunodeficiency characterized by recurrent sinopulmonary and herpes simplex virus infections and poor responses to immunization.
      SIMILARITY: Belongs to the peptidase C14 family. & Contains 2 DED (death effector) domains.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -20 °C. Stable for 12 months from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Caspase-8 2470351
      Anti-Caspase-8 (Rabbit monoclonal) - 2398831 2398831
      Anti-Caspase-8 (Rabbit monoclonal) - 2436326 2436326
      Anti-Caspase-8 (Rabbit monoclonal) - 2154047 2154047
      Anti-Caspase-8 (Rabbit monoclonal) - 2205909 2205909
      Anti-Caspase-8 (Rabbit monoclonal) - 2208754 2208754
      Anti-Caspase-8 (Rabbit monoclonal) - 2283804 2283804
      Anti-Caspase-8 (Rabbit monoclonal) - DAM1443867 DAM1443867
      Anti-Caspase-8 (Rabbit monoclonal) - DAM1444585 DAM1444585
      Anti-Caspase-8 - DAM1410667 DAM1410667

      References

      Reference overviewPub Med ID
      Velogenic newcastle disease virus as an oncolytic virotherapeutics: in vitro characterization.
      Rajiv Kumar,Ashok K Tiwari,Uttara Chaturvedi,G Ravi Kumar,Aditya P Sahoo,R S Rajmani,Lovleen Saxena,Shikha Saxena,Sangeeta Tiwari,Sudesh Kumar
      Applied biochemistry and biotechnology  167  2012

      Show Abstract
      22644640 22644640
      FLICE is activated by association with the CD95 death-inducing signaling complex (DISC)
      Medema, J P, et al
      EMBO J, 16:2794-804 (1997)  1997

      9184224 9184224
      Involvement of MACH, a novel MORT1/FADD-interacting protease, in Fas/APO-1- and TNF receptor-induced cell death.
      Boldin, M P, et al.
      Cell, 85: 803-15 (1996)  1996

      Show Abstract
      8681376 8681376
      Molecular ordering of the Fas-apoptotic pathway: the Fas/APO-1 protease Mch5 is a CrmA-inhibitable protease that activates multiple Ced-3/ICE-like cysteine proteases
      Srinivasula, S M, et al
      Proc Natl Acad Sci USA, 93:14486-91 (1996)  1996

      8962078 8962078
      FLICE, a novel FADD-homologous ICE/CED-3-like protease, is recruited to the CD95 (Fas/APO-1) death--inducing signaling complex
      Muzio, M, et al
      Cell, 85:817-27 (1996)  1996

      8681377 8681377

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      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies