05-572 | Anti-Caspase 9 Antibody, clone 96-2-22

100 µg  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMPurifiedMonoclonal Antibody
      Catalogue Number05-572
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Caspase 9 Antibody, clone 96-2-22
      Product Information
      • Positive Control Included: Non-Stim A431 Cell Lysate (12-301)
      PresentationProtein A Purified immunoglobulin in Protein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
      ApplicationThis Anti-Caspase 9 Antibody, clone 96-2-22 is validated for use in WB for the detection of Caspase 9.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenThe N-terminal fragment of human Caspase 9 (amino acid residues 1-134)
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityRecognizes the proform and the active cleaved form of Caspase 9.
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. Alternative splicing results in two transcript variants which encode different isoforms.
      Gene Symbol
      • CASP9
      • CASP-9
      • ICE-LAP6
      • MCH6
      • APAF3
      • CASPASE-9c
      • OTTHUMP00000044594
      • APAF-3
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P55211 # Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
      SIZE: 416 amino acids; 46281 Da
      SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 35 kDa (p35) and a 10 kDa (p10) subunit. Caspase-9 and APAF1 bind to each other via their respective NH2-terminal CED-3 homologous domains in the presence of cytochrome C and ATP. Interacts with the inhibitors BIRC2, BIRC4, BIRC5 and BIRC7.
      TISSUE SPECIFICITY: Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues.
      PTM: Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase- 3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
      SIMILARITY: SwissProt: P55211 ## Belongs to the peptidase C14 family. & Contains 1 CARD domain.
      Molecular Weight46/34 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from A431, Raji, or HFF cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Caspase 9, clone 96-2-22 2476951
      Anti-Caspase 9, clone 96-2-22 (mouse monoclonal IgG) 3051265
      Anti-Caspase 9, clone 96-2-22 - 20080 20080
      Anti-Caspase 9, clone 96-2-22 - 21577 21577
      Anti-Caspase 9, clone 96-2-22 - 23678 23678
      Anti-Caspase 9, clone 96-2-22 - 2952654 2952654
      Anti-Caspase 9, clone 96-2-22 - DAM1647092 DAM1647092
      Anti-Caspase 9, clone 96-2-22_2839442 2839442


      Reference overviewApplicationPub Med ID
      Caspase-1 promotes Epstein-Barr virus replication by targeting the large tegument protein deneddylase to the nucleus of productively infected cells.
      Gastaldello, S; Chen, X; Callegari, S; Masucci, MG
      PLoS pathogens  9  e1003664  2013

      Show Abstract
      24130483 24130483
      BITC Sensitizes Pancreatic Adenocarcinomas to TRAIL-induced Apoptosis.
      Wicker CA, Sahu RP, Kulkarni-Datar K, Srivastava SK, Brown TL
      Cancer Growth Metastasis  2009  45-55.  2010

      Show Abstract Full Text Article
      20559452 20559452
      Heat shock protein inhibitors increase the efficacy of measles virotherapy.
      Liu, C; Erlichman, C; McDonald, CJ; Ingle, JN; Zollman, P; Iankov, I; Russell, SJ; Galanis, E
      Gene therapy  15  1024-34  2008

      Show Abstract
      18356818 18356818
      Cell cycle arrest and proapoptotic effects of the anticancer cyclodepsipeptide serratamolide (AT514) are independent of p53 status in breast cancer cells.
      Vanessa Soto-Cerrato, Beatriz Montaner, Marc Martinell, Marta Vilaseca, Ernest Giralt, Ricardo Pérez-Tomás
      Biochemical pharmacology  71  32-41  2005

      Show Abstract
      16298346 16298346
      Proapoptotic, antimigratory, antiproliferative, and antiangiogenic effects of commercial C-reactive protein on various human endothelial cell types in vitro: implications of contaminating presence of sodium azide in commercial preparation.
      Liu, C; Wang, S; Deb, A; Nath, KA; Katusic, ZS; McConnell, JP; Caplice, NM
      Circulation research  97  135-43  2005

      Show Abstract
      15976313 15976313
      Inhibition of integrin-linked kinase/protein kinase B/Akt signaling: mechanism for ganglioside-induced apoptosis
      Wang, X. Q., et al
      J Biol Chem, 276:44504-11 (2001)  2001

      Immunoprecipitation, Immunoblotting (Western)11577096 11577096
      Caspase-9 and APAF-1 form an active holoenzyme.
      Rodriguez, J and Lazebnik, Y
      Genes Dev., 13: 3179-84 (1999)  1999

      Show Abstract
      10617566 10617566
      Oncogene-dependent apoptosis is mediated by caspase-9.
      Fearnhead, H O, et al.
      Proc. Natl. Acad. Sci. U.S.A., 95: 13664-9 (1998)  1998

      Show Abstract
      9811857 9811857

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      Included Positive Control

      Catalogue Number Description  
      12-301 Non-Stimulated A431 Cell Lysate Show Pricing & Availability

      Product Families


      Life Science Research > Antibodies and Assays > Primary Antibodies