05-613 | Anti-β-Catenin Antibody, clone 2H4A7

05-613
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RIP, WB, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-613
      Replaces04-1002
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-β-Catenin Antibody, clone 2H4A7
      References
      Product Information
      FormatPurified
      PresentationProtein G purified IgG in buffer containing 0.1M Tris-Glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide
      Applications
      ApplicationDetect β-Catenin also known as beta catenin with Anti-β-Catenin Antibody, clone 2H4A7 (Mouse Monoclonal Antibody), that has been demonstrated to work in IP, WB & IHC.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Immunohistochemistry
      Biological Information
      Immunogenfusion protein corresponding to residues 1-100 of human β-Catenin
      Cloneclone 2H4A7
      HostMouse
      Specificityβ-Catenin
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryBeta-catenin is an adherens junction protein. Adherens junctions (AJs; also called the zonula adherens) are critical for the establishment and maintenance of epithelial layers, such as those lining organ surfaces. AJs mediate adhesion between cells, communicate a signal that neighboring cells are present, and anchor the actin cytoskeleton. In serving these roles, AJs regulate normal cell growth and behavior. At several stages of embryogenesis, wound healing, and tumor cell metastasis, cells form and leave epithelia. This process, which involves the disruption and reestablishment of epithelial cell-cell contacts, may be regulated by the disassembly and assembly of AJs. AJs may also function in the transmission of the 'contact inhibition' signal, which instructs cells to stop dividing once an epithelial sheet is complete.[supplied by OMIM]
      Gene Symbol
      • CTNNB1
      • CTNNB
      • FLJ25606
      • Beta-catenin
      • PRO2286
      • FLJ37923
      • DKFZp686D02253
      Purification MethodProtein G Chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P35222 # Involved in the regulation of cell adhesion and in signal transduction through the Wnt pathway.
      SIZE: 781 amino acids; 85497 Da
      SUBUNIT: Two separate pools are found in the cytoplasm: one is PSEN1/cadherin/catenin complex which anchors to the actin cytoskeleton. The other pool is part of a large complex containing AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. Wnt- dependent activation of DVL antagonizes the action of GSK3B. When GSK3B activity is inhibited the complex dissociates, CTNNB1 is dephosphorylated and is no longer targeted for destruction. The stabilized protein translocates to the nucleus, where it binds TCF/LEF-1 family members, TBP, BCL9 and possibly also RUVBL1 and DUPLIN. Binds CTNNBIP and EP300. CTNNB1 forms a ternary complex with LEF1 and EP300 that is disrupted by CTNNBIP1 binding (By similarity). Interacts with the PDZ domain of TAX1BP3; this interaction inhibits the transcriptional activity of CTNNB1 (By similarity). Interacts with AJAP1, BAIAP1, CARM1, CTNNA3, CXADR and PCDH11Y. Binds SLC9A3R1. Interacts with GLIS2. Interacts with SLC30A9 (By similarity).
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 promotes nuclear translocation.
      TISSUE SPECIFICITY: Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheats. Expressed in colon.
      PTM: Phosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase. Phosphorylation proceeds then from Thr- 41 to Ser-37 and Ser-33. & EGF stimulates tyrosine phosphorylation. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding. & Ubiquitinated by a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1A, APC and TBL1X (Probable). Its ubiquitination leads to its subsequent proteasomal degradation.
      DISEASE: "SwissProt: P35222 # Activating mutations in CTNNB1 have oncogenic activity resulting in tumor development. Somatic mutations are found in various tumor types, including colon cancers, ovarian and prostate carcinomas, hepatoblastoma (HB), hepatocellular carcinoma (HCC). HBs are malignant embryonal tumors mainly affecting young children in the first three years of life. & Defects in CTNNB1 are a cause of pilomatrixoma (PTR) [MIM:132600]; a common benign skin tumor. & Defects in CTNNB1 are a cause of medulloblastoma (MDB) [MIM:155255]. MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children. & A chromosomal rearrangement involving CTNNB1 may be a cause of salivary gland pleiomorphic adenomas (PA) [181030]. Pleiomorphic adenomas are the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1."
      SIMILARITY: SwissProt: P35222 ## Belongs to the beta-catenin family. & Contains 12 ARM repeats.
      Molecular Weight92kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from A431, mouse 3T3/A31 or rat L6 cell lysates
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable 2 years at 2-8°C
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-b-Catenin, NT, clone 2H4A7 (mouse monoclonal IgG) - 2106955 2106955
      Anti-b-Catenin, NT, clone 2H4A7 (mouse monoclonal IgG) - 2201570 2201570
      Anti-b-Catenin, NT, clone 2H4A7 (mouse monoclonal IgG) - DAM1416512 DAM1416512
      Anti-b-Catenin, NT, clone 2H4A7 - 1984835 1984835
      Anti-b-Catenin, NT, clone 2H4A7 - 2183662 2183662
      Anti-b-Catenin, NT, clone 2H4A7 - DAM1612182 DAM1612182
      Anti-b-Catenin, NT, clone 2H4A7 - DAM1780359 DAM1780359
      Anti-b-Catenin, NT, clone 2H4A7 - JBC1831390 JBC1831390
      Anti-b-Catenin, NT, clone 2H4A7 - NG1951650 NG1951650
      Anti-b-Catenin, NT, clone 2H4A7 -2705046 2705046

      References

      Reference overviewApplicationPub Med ID
      Acid ceramidase (ASAH1) is a global regulator of steroidogenic capacity and adrenocortical gene expression.
      Lucki, NC; Bandyopadhyay, S; Wang, E; Merrill, AH; Sewer, MB
      Molecular endocrinology (Baltimore, Md.)  26  228-43  2012

      Show Abstract
      22261821 22261821
      Inhibition of GSK3β improves hippocampus-dependent learning and rescues neurogenesis in a mouse model of fragile X syndrome.
      Guo, W; Murthy, AC; Zhang, L; Johnson, EB; Schaller, EG; Allan, AM; Zhao, X
      Human molecular genetics  21  681-91  2012

      Show Abstract
      Western Blotting22048960 22048960
      Matrix Metalloproteinase-9 Silencing by RNA Interference Triggers the Migratory-adhesive Switch in Ewing's Sarcoma Cells
      Sanceau, J., et al
      J Biol Chem, 278:36537-46 (2003)  2003

      Immunofluorescence12847101 12847101

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      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies