06-114 | Anti-Cathepsin D Antibody

06-114
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number06-114
      DescriptionAnti-Cathepsin D Antibody
      Alternate Names
      • cathepsin D
      • cathepsin D (lysosomal aspartyl protease)
      • lysosomal aspartyl peptidase
      • lysosomal aspartyl protease
      Background InformationCathepsin D is synthesized as a 54kDa precursor which is proteolytically processed to an intermediate 48kDa single chain which matures into more stable 34kDa and 14kDa two chain form. It is an estrogen-regulated lysosomal protease that has been suggested to facilitate cancer cell migration and invasion by digesting the basement membrane, extracellular matrix, and connective tissue. Because of its mitogenic and proteolytic activities, it has been implicated as a prognostic marker in many tumor types, especially breast cancer. Cathepsin D is expressed in epithelial cells as well as in macrophages. Immunoreactivity of cancer cells and tissue infiltrating macrophages must be recorded separately.
      References
      Product Information
      FormatAffinity Purified
      Control
      • A431 cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Applications
      ApplicationAnti-Cathepsin D Antibody is an antibody against Cathepsin D for use in WB & IC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: 1:500 dilution from a previous lot detected Cathepsin D in A431 and HeLa cell lystes.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to human Cathepsin D at the heavy chain.
      EpitopeHeavy Chain
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes Cathepsin D.
      Species Reactivity
      • Human
      Species Reactivity NoteProven to react with human. 84% sequence homology to mouse and rat.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a lysosomal aspartyl protease composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. This proteinase, which is a member of the peptidase C1 family, has a specificity similar to but narrower than that of pepsin A. Transcription of this gene is initiated from several sites, including one which is a start site for an estrogen-regulated transcript. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease.
      Gene Symbol
      • CTSD
      • CLN10
      • CPSD
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.

      SIZE: 412 amino acids; 44552 Da

      SUBUNIT: Consists of a light chain and a heavy chain.

      SUBCELLULAR LOCATION: Lysosome. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

      DISEASE: Defects in CTSD are the cause of neuronal ceroid lipofuscinosis 10 (CLN10) [MIM:610127]; also known as neuronal ceroid lipofuscinosis due to cathepsin D deficiency. The neuronal ceroid lipofuscinosis are a group of progressive neurodegenerative diseases in children and in adults, characterized by visual and mental decline, motor disturbance, epilepsy and behavioral changes.

      SIMILARITY: Belongs to the peptidase A1 family.
      Molecular WeightObserved molecular weight at ~58 kDa. Protein is glycosylated and may run slightly higher than predicted.
      Predicted molecular weight of the active form is ~46 kDa and ~ 54 kDa for the precursor form.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in A431 cell lysate.

      Western Blot Analysis: 1 µg/mL of this antibody detected Cathepsin D on 10 µg of A431 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Cathepsin D - 2376458 2376458
      Anti-Cathepsin D - 1963615 1963615
      Anti-Cathepsin D - 2068450 2068450
      Anti-Cathepsin D - 2197265 2197265
      Anti-Cathepsin D - 2492774 2492774
      Anti-Cathepsin D - 2541558 2541558
      Anti-Cathepsin D - NG1740998 NG1740998
      Anti-Cathepsin D - NRG1662098 NRG1662098
      Anti-Cathepsin D -2556285 2556285
      Anti-Cathepsin D -2683858 2683858

      References

      Reference overviewPub Med ID
      Relationship among placental cadmium, lead, zinc, and copper levels in smoking pregnant women.
      Türkan Kutlu,A Alev Karagozler,Engin M Gozukara
      Biological trace element research  114  2006

      Show Abstract
      17205983 17205983