04-966 | Anti-Cby1 Antibody, clone 8-2

04-966
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, HICC, IPMPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-966
      DescriptionAnti-Cby1 Antibody, clone 8-2
      Alternate Names
      • ARPP-binding protein
      • Cytosolic leucine-rich protein
      • PKD2 interactor, Golgi and endoplasmic reticulum-associated 1
      • PKD2 interactor, golgi and endoplasmic reticulum associated 1
      • chibby homolog 1 (Drosophila)
      • chromosome 22 open reading frame 2
      • coiled-coil protein PIGEA-14 2
        polycystin-2 interactor, Golgi- and endoplasmic reticulum-associated protein, 14 kDa
      Background InformationChibby (Cby) is a β-catenin antagonist that is evolutionarily conserved from fly to human. Cby interacts with β-catenin in a manner that competes with TCF/LEF, and thus represses β-catenin-mediated gene activation. Cby and 14-3-3 protein form a trimolecular complex with β-catenin. Cby and 14-3-3 collaborate to promote cytoplasmic localization of β-catenin and thereby inhibit β-catenin signalling. Cby acts, in concert with 14-3-3 proteins, to facilitate nuclear export of β-catenin and thereby antagonize β-catenin signalling. Therefore, the inhibition of β-catenin signalling by Cby involves at least two distinct molecular mechanisms: competing with TCF/LEF transcription factors for β-catenin in the nucleus and facilitating nuclear export of β-catenin through interaction with 14-3-3 following its phosphorylation by AKT/PKB.
      References
      Product Information
      FormatPurified
      Control
      • NIH/3T3, A431, HeLa, and C2C12 cells
      PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Applications
      ApplicationThis Anti-Cby1 Antibody, clone 8-2 is validated for use in IC, IP for the detection of Cby1.
      Key Applications
      • Immunocytochemistry
      • Immunoprecipitation
      Application NotesImmunocytochemistry Analysis: 1:500 dilution from a previous lot detected Cby1 in NIH/3T3, A431, HeLa, and C2C12 cells.

      Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP. (Feng-Qian, L., et al. (2008). J. Cell Biol. 181(7):1141–1154.)

      Western Blot Analysis: Western Blot is difficult to detect on endogenous lysates but has been shown to work in over expressed cell lines.
      Biological Information
      ImmunogenRecombinant protein corresponding to human Cby1.
      EpitopeUnknown
      Clone8-2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes Cby1.
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      • Human
      Species Reactivity NoteProven to react with human and mouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryBeta-catenin is a transcriptional activator and oncoprotein involved in the development of several cancers. The protein encoded by this gene interacts directly with the C-terminal region of beta-catenin, inhibiting oncogenic beta-catenin-mediated transcriptional activation by competing with transcription factors
      for binding to beta-catenin. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
      Gene Symbol
      • CBY1
      • ARB1
      • CBY
      • Cby
      • Chibby
      • PGEA1
      • PIGEA-14
      • PIGEA14
      • arb1
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Inhibits the Wnt/Wingless pathway by binding to beta-catenin and inhibiting beta-catenin-mediated transcriptional activation through competition with TCF/LEF transcription factors. Has also been shown to play a role in regulating the intracellular trafficking of polycystin-2/PKD2 and possibly of other intracellular proteins. Promotes adipocyte and cardiomyocyte differentiation.

      SUBUNIT STRUCTURE: Homodimer; interacts with polycystin-2/PKD2 and GM130. Interacts with the C-terminal region of beta-catenin.

      SUBCELLULAR LOCATION: Nucleus speckle. Golgi apparatus › trans-Golgi network. Note: Nuclear, in a punctate manner. Also found in the trans-Golgi.

      TISSUE SPECIFICITY: Widely expressed. Expressed at higher levels in heart, skeletal muscle, kidney and placenta. Also found in brain, lung, liver and testis. Significantly down-regulated in thyroid and metastatic uterine tumors.

      MISCELLANEOUS: Chibby is Japanese for 'small'; the gene was so named for the RNAi phenotype seen in flies.

      SEQUENCE SIMILARITIES: Belongs to the chibby family.
      Molecular Weight ~14 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance Evaluated by Immunocytochemistry Analysis on NIH/3T3 cells.

      Immunocytochemistry Analysis: This antibody positively stains the cytoplasm. The nucleus is also stained with less intensity.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications