05-743 | Anti-Cdc25A Antibody, clone DCS120

05-743
100 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RIP, WB, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-743
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Cdc25A Antibody, clone DCS120
      References
      Product Information
      FormatPurified
      Presentation10mM PBS, pH 7.4
      Applications
      ApplicationAnti-Cdc25A Antibody, clone DCS120 is a high quality Mouse Monoclonal Antibody for the detection of Cdc25A & has been validated in IP, WB & IHC.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Immunohistochemistry
      Biological Information
      ImmunogenPurified recombinant cdc25A
      Cloneclone DCS120
      HostMouse
      Specificitycdc25A
      IsotypeIgG2a
      Species Reactivity
      • Human
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryCDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene.
      Gene Symbol
      • CDC25A
      • CDC25A2
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P30304 # Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDC2 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.
      SIZE: 524 amino acids; 59087 Da
      SUBUNIT: Interacts with CCNB1/cyclin B1. Interacts with YWHAE/14- 3-3 epsilon when phosphorylated. Interacts with CUL1 specifically when CUL1 is neddylated and active. Interacts with BTRC/BTRCP1 and FBXW11/BTRCP2. Interactions with CUL1, BTRC and FBXW11 are enhanced upon DNA damage.
      DOMAIN: SwissProt: P30304 The phosphodegron motif mediates interaction with specific F-box proteins when phosphorylated. Putative phosphorylation sites at Ser-79 and Ser-82 appear to be essential for this interaction.
      PTM: Phosphorylated by CHEK1 on Ser-76, Ser-124, Ser-178, Ser-279, Ser-293 and Thr-507 during checkpoint mediated cell cycle arrest. Also phosphorylated by CHEK2 on Ser-124, Ser-279, and Ser-293 during checkpoint mediated cell cycle arrest. Phosphorylation on Ser-178 and Thr-507 creates binding sites for YWHAE/14-3-3 epsilon which inhibits CDC25A. Phosphorylation on Ser-76, Ser-124, Ser- 178, Ser-279 and Ser-293 may also promote ubiquitin-dependent proteolysis of CDC25A. & Ubiquitinated. Association with the F-box proteins BTRC and FBXW11 targets the protein for ubiquitination by CUL1 and proteolysis by the ubiquitin-dependent proteasome pathway.
      SIMILARITY: Belongs to the MPI phosphatase family. & Contains 1 rhodanese domain.
      Molecular Weight~65kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on nuclear extract from HeLa cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-cdc25A, clone DCS120 (mouse monoclonal IgG2a) - 1995580 1995580
      Anti-cdc25A, clone DCS120 (mouse monoclonal IgG2a) -2697635 2697635
      Anti-cdc25A, clone DCS120 - 27295 27295
      Anti-cdc25A, clone DCS120 - 27773 27773
      Anti-cdc25A, clone DCS120 -2460923 2460923
      Anti-cdc25A, clone DCS120 -2574237 2574237
      Anti-cdc25A, clone DCS120 -2585764 2585764
      Anti-cdc25A, clone DCS120 -2642173 2642173

      References

      Reference overviewPub Med ID
      Sequence-specific activation of TAK1-D by short double-stranded RNAs induces apoptosis in NCI-H460 cells.
      Kodym, R; Kodym, E; Story, MD
      RNA (New York, N.Y.)  14  535-42  2008

      Show Abstract Full Text Article
      18230764 18230764

      Related Products & Applications

      Included Positive Control

      Catalogue Number Description  
      12-309 HeLa Nuclear Extract Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies