05-507 | Anti-Cdc25C Antibody, clone TC-15

05-507
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MIP, WB, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-507
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Cdc25C Antibody, clone TC-15
      Background InformationThe activity of cyclin-dependent kinases is regulated by their phosphorylation status, which is controlled by the antagonistic action of CDC25 phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B and CDC25C. CDC25C is a ~56 kDa dual specific protein phosphatase that controls entry into mitosis by regulating the dephosphorylation of the Cdk1/cyclin B complex. Phosphorylation of Cdc25C creates a binding site for 14-3-3 protein which inhibits Cdc25C. This prevents activation of the Cdk1/cyclin B complex and prevents mitotic entry. Cdc25C is phosphorylated by checkpoint kinases throughout interphase but becomes dephosphorylated during mitosis. Cdc25C is also regulated by p53 via a p53 response element in its promoter, and it is predominantly expressed in the G2 phase of the cell cycle.
      References
      Product Information
      FormatPurified
      Control
      • HeLa nuclear extract
      Presentation0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
      Applications
      ApplicationDetect Cdc25C with Anti-Cdc25C Antibody, clone TC-15 (Mouse Monoclonal Antibody), that has been shown to work in IP, WB, ICC.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Immunocytochemistry
      Biological Information
      ImmunogenGST fusion-protein corresponding to residues 1-258 of human cdc25C. Epitope mapped to residues 222-246.
      Cloneclone TC-15
      HostMouse
      Specificitycdc25C
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known.
      Gene Symbol
      • CDC25C
      • CDC25
      Purification MethodProtein G Chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P30307 # Functions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It directly dephosphorylates CDC2 and activate its kinase activity.
      SIZE: 473 amino acids; 53365 Da
      SUBUNIT: Interacts with HIV-1 Vpr, thereby inactivating CDC25C phosphatase activity.
      SUBCELLULAR LOCATION: Nucleus.
      DEVELOPMENTAL STAGE: Expressed predominantly in G2 phase.
      PTM: Phosphorylated by CHK1 on Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase.
      SIMILARITY: SwissProt: P30307 ## Belongs to the MPI phosphatase family. & Contains 1 rhodanese domain.
      Molecular Weight58kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on HeLa nuclear extract
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Histone H3, CT, pan, clone A3S -2554884 2554884
      Anti-cdc25C, clone TC-15 (mouse monoclonal IgG1k) - 2359852 2359852
      Anti-cdc25C, clone TC-15 - 18463 18463
      Anti-cdc25C, clone TC-15 - 22623 22623
      Anti-cdc25C, clone TC-15 - 31395 31395
      Anti-cdc25C, clone TC-15 - DAM1692597 DAM1692597
      Anti-cdc25C, clone TC-15 - JBC1841201 JBC1841201
      Anti-cdc25C, clone TC-15 -2556926 2556926
      Anti-cdc25C, clone TC-15 -2719878 2719878
      Anti-cdc25C, clone TC-15 -2891855 2891855

      References

      Reference overviewPub Med ID
      DNA-Dependent Protein Kinase and Ataxia Telangiectasia Mutated (ATM) Promote Cell Survival in Response to NK314, a Topoisomerase II{alpha} Inhibitor.
      Guo L, Liu X, Jiang Y, Nishikawa K, Plunkett W
      Molecular pharmacology  80  321-7. Epub 2011 May 5.  2011

      Show Abstract
      21546556 21546556
      Molecular basis for G2 arrest induced by 2'-C-cyano-2'-deoxy-1-beta-D-arabino-pentofuranosylcytosine and consequences of checkpoint abrogation.
      Liu, X; Guo, Y; Li, Y; Jiang, Y; Chubb, S; Azuma, A; Huang, P; Matsuda, A; Hittelman, W; Plunkett, W
      Cancer research  65  6874-81  2005

      Show Abstract
      16061671 16061671
      Mitotic and G2 checkpoint control: regulation of 14-3-3 protein binding by phosphorylation of Cdc25C on serine-216.
      Peng, C Y, et al.
      Science, 277: 1501-5 (1997)  1997

      Show Abstract
      9278512 9278512

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies