05-354 | Anti-Clusterin α chain (human) Antibody, clone 41D

05-354
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICC, IHC, IH(P), WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-354
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Clusterin α chain (human) Antibody, clone 41D
      Alternate Names
      • Apolipoprotein J
      • Complement cytolysis inhibitor
      • Complement-associated protein SP-40,40
      • Ku70-binding protein 1
      • Testosterone-repressed prostate message 2
      • aging-associated protein 4
      • clusterin
      • complement lysis inhibitor
      • complement-associated protein SP-40
      • sulfated glycoprotein 2
      Background InformationClusterin is also known as apolipoprotein J, complement lysis inhibitor, gp80, glycoprotein III, SGP-2, SP 40-40, TRPM2 and T64. It is a ubiquitous multifunctional protein of 80kD comprising of two disulfide-linked subunits, alpha and beta, and capable of interacting with a broad range of molecules. It is implicated in numerous biological processes including sperm maturation, lipid transport, regulation of the complement cascade, membrane recycling, cell death, immune regulation, cell adhesion and morphological transformation. In pathological conditions, it is an amyloid associated protein co-localising with fibrillar deposits in systemic and localised amyloid disorders. In Alzheimer ’s disease, clusterin is present in amyloid plaques and cerebrovascular deposits. In breast cancers, clusterin expression was found to correlate with tumour size. Clusterin is also implicated with endometrial cancers, myocardial infarction, renal diseases and atherosclerosis.
      References
      Product Information
      FormatPurified
      Control
      • HeLa cell lysate.
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, with 0.05% sodium azide. Frozen at -20°C.
      Applications
      ApplicationAnti-Clusterin α chain (human) Antibody, clone 41D is an antibody against Clusterin α chain (human) for use in IC, IH, IH(P) & WB.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry:
      Previous lots of this antibody at 5 µg/mL detected clusterin in paraffin embedded human brain sections.
      Biological Information
      ImmunogenNative clusterin purified from human serum. Clone 41D.
      Clone41D
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes human clusterin α chain, Mr 40 kDa, and precursor protein.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CLU
      • SP-40
      • MGC24903
      • NA1/NA2
      • SGP-2
      • AAG4
      • KUB1
      • APOJ
      • CLI
      • TRPM-2
      • TRPM2
      • Apo-J
      • SGP2
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P10909 # Not yet clear. It is known to be expressed in a variety of tissues and it seems to be able to bind to cells, membranes and hydrophobic proteins. It has been associated with programmed cell death (apoptosis).
      SIZE: 449 amino acids; 52495 Da
      SUBUNIT: Antiparallel disulfide-linked heterodimer. Interacts with CLUAP1.
      SUBCELLULAR LOCATION: Secreted.
      SIMILARITY: SwissProt: P10909 ## Belongs to the clusterin family.
      Molecular Weight40 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated on RIPA lysates from HeLa cells or MCF-7 cell lysates.

      Western Blot Analysis:
      0.5-2 µg/mL of a previous lot detected human clusterin α chain in RIPA lysates from HeLa cells; previous lots detected the protein in human serum and MCF-7 cell lysates
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations:
      Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Clusterin chain (human), clone 41D - 1984809 1984809
      Anti-Clusterin chain (human), clone 41D - 2035503 2035503
      Anti-Clusterin chain (human), clone 41D - 2329120 2329120
      Anti-Clusterin chain (human), clone 41D - DAM1614903 DAM1614903
      Anti-Clusterin chain (human), clone 41D - DAM1778023 DAM1778023
      Anti-Clusterin chain (human), clone 41D - JBC1900495 JBC1900495
      Anti-Clusterin #225; chain (human), clone 41D - DAM1460168 DAM1460168
      Anti-Clusterin #945; chain (human), clone 41D - 2137076 2137076
      Anti-Clusterin a chain (human), clone 41D (mouse monoclonal IgG1k) - DAM1413898 DAM1413898
      Anti-Clusterin alpha; chain (human), clone 41D - 0609040503 0609040503

      References

      Reference overviewApplicationPub Med ID
      Anti-cancer activity of glucosamine through inhibition of N-linked glycosylation.
      Chesnokov, V; Gong, B; Sun, C; Itakura, K
      Cancer cell international  14  45  2014

      Show Abstract
      24932134 24932134
      Plasma clusterin concentration is associated with longitudinal brain atrophy in mild cognitive impairment.
      Thambisetty, M; An, Y; Kinsey, A; Koka, D; Saleem, M; Güntert, A; Kraut, M; Ferrucci, L; Davatzikos, C; Lovestone, S; Resnick, SM
      NeuroImage  59  212-7  2012

      Show Abstract
      21824521 21824521
      Plasma proteomic alterations in non-human primates and humans after chronic alcohol self-administration.
      Willard M Freeman,Heather D Vanguilder,Elizabeth Guidone,John H Krystal,Kathleen A Grant,Kent E Vrana
      The international journal of neuropsychopharmacology / official scientific journal of the Collegium Internationale Neuropsychopharmacologicum (CINP)  14  2011

      Show Abstract
      21303580 21303580
      Clusterin, a haploinsufficient tumor suppressor gene in neuroblastomas.
      Olesya Chayka,Daisy Corvetta,Michael Dews,Alessandro E Caccamo,Izabela Piotrowska,Giorgia Santilli,Sian Gibson,Neil J Sebire,Nourredine Himoudi,Michael D Hogarty,John Anderson,Saverio Bettuzzi,Andrei Thomas-Tikhonenko,Arturo Sala
      Journal of the National Cancer Institute  101  2009

      Show Abstract Full Text Article
      19401549 19401549
      Clusterin in cerebrospinal fluid: analysis of carbohydrates and quantification of native and glycosylated forms.
      A-M Nilselid, Pia Davidsson, Katarina Nägga, Niels Andreasen, Pam Fredman, Kaj Blennow
      Neurochemistry international  48  718-28  2006

      Show Abstract
      16490286 16490286
      Cell detachment and apoptosis induction of immortalized human prostate epithelial cells are associated with early accumulation of a 45 kDa nuclear isoform of clusterin
      Caccamo, A. E., et al
      Biochem J, 382:157-68 (2004)  2004

      Immunoblotting (Western), Immunohistochemistry (Tissue)15139853 15139853
      Clusterin overexpression in both malignant and nonmalignant prostate epithelial cells induces cell cycle arrest and apoptosis
      Scaltriti, M., et al
      Br J Cancer, 91:1842-50 (2004)  2004

      Immunocytochemistry15494717 15494717
      Involvement of clusterin in 15-deoxy-delta12,14-prostaglandin J2-induced vascular smooth muscle cell differentiation.
      Yoshikazu Miwa, Fumi Takahashi-Yanaga, Sachio Morimoto, Toshiyuki Sasaguri
      Biochemical and biophysical research communications  319  163-8  2004

      Show Abstract
      15158456 15158456
      Modulation of different clusterin isoforms in human colon tumorigenesis.
      Pucci, S; Bonanno, E; Pichiorri, F; Angeloni, C; Spagnoli, LG
      Oncogene  23  2298-304  2004

      Show Abstract
      14755245 14755245
      Characterization of a vitamin D3-resistant MCF-7 cell line
      Narvaez, C J, et al
      Endocrinology, 137:400-9 (1996)  1996

      8593782 8593782

      FAQ

      QuestionAnswer
      I would like to use this monoclonal antibody on mouse tissue, are there any special procedures I should follow?To reduce the background interaction between the monoclonal antibody and the mouse tissue, you will need to perform antigen retrieval and use a special secondary antibody. For antigen retrieval, prepare a citrate buffer by mixing 19ml of 0.1M citric acid and 82ml of 0.1M sodium citrate to make 1000ml of buffer. Adjust the pH to 6.0 before each use. Add buffer to your slide. Using an 800 watt microwave oven with rotation, microwave for 7.5 min at high, 5 min at 50% (3 cycles), checking the slide at the end of each cycle to see if the solution needs to be replaced, adding water if needed. For the secondary antibody, use a biotinylated isotype specific secondary IGG (IGG-1) and a 3% donkey serum block. This will help reduce the background issues using mouse monoclonals.

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies