07-300 | Anti-Cofilin 2 Antibody

07-300
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RWBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number07-300
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Cofilin 2 Antibody
      References
      Product Information
      FormatPurified
      Presentation0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
      Applications
      ApplicationThis Anti-Cofilin 2 Antibody is validated for use in WB for the detection of Cofilin 2.
      Key Applications
      • Western Blotting
      Biological Information
      Immunogenpeptide corresponding to amino acids 136-152 (C-QVNGLDDIKDRSTLGEK) of human Cofilin 2
      HostRabbit
      SpecificityCofilin 2
      IsotypeIgG
      Species Reactivity
      • Human
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryCofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. (Gillett et al., 1996 [PubMed 8800436]). Cofilin-2 is a member of the AC group of proteins that also includes cofilin-1 (CFL1) and destrin (DSTN; MIM 609114), all of which regulate actin-filament dynamics (Bamburg et al., 1999 [PubMed 10461190]; Maciver and Hussey, 2002). The CFL2 gene encodes a skeletal muscle-specific isoform (Vartiainen et al., 2002 [PubMed 11809832]) localized to the thin filaments, where it exerts its effect on actin, in part through interactions with tropomyosins (Ono and Ono, 2002 [PubMed 11901171]).[supplied by OMIM]
      Gene Symbol
      • CFL2
      • NEM7
      • Cofilin-2
      Purification MethodProtein A chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q9Y281 # Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods (By similarity).
      SIZE: 166 amino acids; 18737 Da
      SUBCELLULAR LOCATION: Nucleus matrix (By similarity). Cytoplasm, cytoskeleton (By similarity).
      TISSUE SPECIFICITY: Isoform CFL2b is expressed predominantly in skeletal muscle and heart, while isoform CFL2a is expressed in various tissues.
      PTM: The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal.
      DISEASE: SwissProt: Q9Y281 # Defects in CFL2 are the cause of nemaline myopathy type 7 (NEM7) [MIM:610687]. Nemaline myopathy (NEM) is a form of congenital myopathy characterized by abnormal thread- or rod-like structures in muscle fibers on histologic examination. The clinical phenotype is highly variable, with differing age at onset and severity.
      SIMILARITY: SwissProt: Q9Y281 ## Belongs to the actin-binding proteins ADF family. & Contains 1 ADF-H domain.
      Molecular Weight21kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from rat L6 cell lysates
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Cofilin 2 2475666
      Anti-Cofilin 2 2842683
      Anti-Cofilin 2 (Rabbit polyclonal IgG) 2955687
      Anti-Cofilin 2 - 22222 22222
      Anti-Cofilin 2 - 32320 32320

      References

      Reference overviewPub Med ID
      MicroRNAs are essential for stretch-induced vascular smooth muscle contractile differentiation via microRNA (miR)-145-dependent expression of L-type calcium channels.
      Turczynska, KM; Sadegh, MK; Hellstrand, P; Swärd, K; Albinsson, S
      The Journal of biological chemistry  287  19199-206  2012

      Show Abstract
      22474293 22474293
      Nitric oxide inhibits endothelin-1-induced neonatal cardiomyocyte hypertrophy via a RhoA-ROCK-dependent pathway.
      Hunter, J Craig, et al.
      J. Mol. Cell. Cardiol., 47: 810-8 (2009)  2009

      Show Abstract
      19799911 19799911
      Differential dependence of stretch and shear stress signaling on caveolin-1 in the vascular wall.
      Albinsson, S; Nordström, I; Swärd, K; Hellstrand, P
      American journal of physiology. Cell physiology  294  C271-9  2008

      Show Abstract
      17989209 17989209
      Stromal cell-derived factor 1alpha activates LIM kinase 1 and induces cofilin phosphorylation for T-cell chemotaxis.
      Nishita, Michiru, et al.
      Mol. Cell. Biol., 22: 774-83 (2002)  2002

      Show Abstract
      11784854 11784854
      Mitosis-dependent phosphorylation and activation of LIM-kinase 1.
      Sumi, Tomoyuki, et al.
      Biochem. Biophys. Res. Commun., 290: 1315-20 (2002)  2002

      Show Abstract
      11812007 11812007

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies