05-180 | Anti-Cortactin (p80/85) Antibody, clone 4F11

05-180
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Av, B, H, Ht, M, RICC, IHC, IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-180
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Cortactin (p80/85) Antibody, clone 4F11
      Background InformationCortactin is present as a p80 and p85 kDa protein in many cell types.  The protein was first identified as being a major substrate of the tyrosine kinase pp60SRC. The main structural feature of the protein is that at the N-terminus there are five and a half 37-amino acid repeats, these appear to be responsible for the actin-binding.  This repeat region occupies about a third of the protein.  The repeat region is followed by a helical region and then a proline rich region and finally at the C-terminus, an SH3 domain.  The proline rich domain is thought to bind the SH2 domain of v-src the viral version of the normal c-src known to phosphorylate cortactin.  Cortactin bundles microfilaments and this bundling is inhibited by tyrosine phosphorylation.  One pathway that activates the pathway leading to tyrosine phosphorylation of cortactin results from a cell adhesion molecule in brain micro-vessel endothelial cells, another is the growth factor, but presumably the very many mechanisms for pp60src activation would also result in cortactin phosphorylation and subsequent loss of actin binding. 
      References
      Product Information
      FormatPurified
      Control
      • Positive Control Included: 3T3 Cell Lysate (12-305) Other controls: PC-12 cell lysate, HeLa whole cell lysate or A-431 whole cell lysate
      PresentationPurified immunoglobulin in 10mM PBS, pH 7.4 with 0.05% sodium azide.
      Applications
      ApplicationAnti-Cortactin (p80/85) Antibody, clone 4F11 is an antibody against Cortactin (p80/85), and has been published and validated for use in IC, IH, IP and WB.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenMixture of affinity-purified tyrosine phosphoproteins from chick embryo fibroblasts expressing activated pp60src
      Clone4F11
      HostMouse
      SpecificityRecognizes Cortactin.
      IsotypeIgG1
      Species Reactivity
      • Avian
      • Bovine
      • Human
      • Hamster
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Two splice variants that encode different isoforms have been identified for this gene.
      Gene Symbol
      • CTTN
      • EMS1
      • Amplaxin
      • FLJ34459
      • 1110020L01Rik
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q14247 # May contribute to the organization of cell structure. The SH3 motif may function as a binding region to cytoskeleton. Tyrosine phosphorylation in transformed cells may contribute to cellular growth regulation and transformation.
      SIZE: 550 amino acids; 61636 Da
      SUBUNIT: Interacts with SHANK2 and SHANK3 via its SH2 domain. Also interacts with FGD1 (By similarity). Interacts with PLXDC2.
      SUBCELLULAR LOCATION: Cytoplasm (By similarity). Cell projection, lamellipodium (By similarity). Cell projection, ruffle (By similarity). Note=Associated with membrane ruffles and lamellipodia (By similarity).
      SIMILARITY: SwissProt: Q14247 ## Contains 7 cortactin repeats. & Contains 1 SH3 domain.
      Molecular Weight80 kDa and 85 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated in mouse 3T3/A31 cell lysates or chicken fibroblast src-transformed cell lysate
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Cortactin (p80/85), clone 4F11 - 2117727 2117727
      Anti-Cortactin (p80/85), clone 4F11 - 2384723 2384723
      Anti-Cortactin (p80/85), clone 4F11 - 2428646 2428646
      Anti-Cortactin (p80/85), clone 4F11 - 13893 13893
      Anti-Cortactin (p80/85), clone 4F11 - 19037 19037
      Anti-Cortactin (p80/85), clone 4F11 - 19406 19406
      Anti-Cortactin (p80/85), clone 4F11 - 1967309 1967309
      Anti-Cortactin (p80/85), clone 4F11 - 2013166 2013166
      Anti-Cortactin (p80/85), clone 4F11 - 21903 21903
      Anti-Cortactin (p80/85), clone 4F11 - 2210407 2210407

      References

      Reference overviewApplicationPub Med ID
      In vivo imaging and characterization of actin microridges.
      Lam, PY; Mangos, S; Green, JM; Reiser, J; Huttenlocher, A
      PloS one  10  e0115639  2015

      Show Abstract
      25629723 25629723
      Overexpression of galectin-7 in mouse epidermis leads to loss of cell junctions and defective skin repair.
      Gendronneau, G; Sanii, S; Dang, T; Deshayes, F; Delacour, D; Pichard, E; Advedissian, T; Sidhu, SS; Viguier, M; Magnaldo, T; Poirier, F
      PloS one  10  e0119031  2015

      Show Abstract
      25741714 25741714
      Hippocampal Cortactin Levels are Reduced Following Spatial Working Memory Formation, an Effect Blocked by Chronic Calpain Inhibition.
      Olson, ML; Ingebretson, AE; Harmelink, KM
      Brain sciences  5  241-57  2015

      Show Abstract
      26103422 26103422
      Gap geometry dictates epithelial closure efficiency.
      Ravasio, A; Cheddadi, I; Chen, T; Pereira, T; Ong, HT; Bertocchi, C; Brugues, A; Jacinto, A; Kabla, AJ; Toyama, Y; Trepat, X; Gov, N; Neves de Almeida, L; Ladoux, B
      Nature communications  6  7683  2015

      Show Abstract
      26158873 26158873
      Fe65 Suppresses Breast Cancer Cell Migration and Invasion through Tip60 Mediated Cortactin Acetylation.
      Sun, Y; Sun, J; Lungchukiet, P; Quarni, W; Yang, S; Zhang, X; Bai, W
      Scientific reports  5  11529  2015

      Show Abstract
      26166158 26166158
      STIM1- and Orai1-mediated Ca(2+) oscillation orchestrates invadopodium formation and melanoma invasion.
      Sun, J; Lu, F; He, H; Shen, J; Messina, J; Mathew, R; Wang, D; Sarnaik, AA; Chang, WC; Kim, M; Cheng, H; Yang, S
      The Journal of cell biology  207  535-48  2014

      Show Abstract
      25404747 25404747
      Capping protein regulatory cycle driven by CARMIL and V-1 may promote actin network assembly at protruding edges.
      Fujiwara, I; Remmert, K; Piszczek, G; Hammer, JA
      Proceedings of the National Academy of Sciences of the United States of America  111  E1970-9  2014

      Show Abstract
      24778263 24778263
      Clathrin light chains are required for the gyrating-clathrin recycling pathway and thereby promote cell migration.
      Majeed, SR; Vasudevan, L; Chen, CY; Luo, Y; Torres, JA; Evans, TM; Sharkey, A; Foraker, AB; Wong, NM; Esk, C; Freeman, TA; Moffett, A; Keen, JH; Brodsky, FM
      Nature communications  5  3891  2014

      Show Abstract
      Immunofluorescence24852344 24852344
      Comparative transcriptomics reveals RhoE as a novel regulator of actin dynamics in bone-resorbing osteoclasts.
      Georgess, D; Mazzorana, M; Terrado, J; Delprat, C; Chamot, C; Guasch, RM; Pérez-Roger, I; Jurdic, P; Machuca-Gayet, I
      Molecular biology of the cell  25  380-96  2014

      Show Abstract
      Immunofluorescence24284899 24284899
      Alternative splice isoforms of small conductance calcium-activated SK2 channels differ in molecular interactions and surface levels.
      Scholl, ES; Pirone, A; Cox, DH; Duncan, RK; Jacob, MH
      Channels (Austin, Tex.)  8  62-75  2014

      Show Abstract
      24394769 24394769

      Technical Info

      Title
      Fluorescent Gelatin Degradation Assays for Investigating Invadopodia Formation

      Newsletters / Publications

      Title
      Cellutions Newsletter: 2011, Vol. 2

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      Alternative Format

      Catalogue Number Description  
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      Included Positive Control

      Catalogue Number Description  
      12-305 3T3/A31 Cell Lysate Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies