05-180-AF647 | Anti-Cortactin (p80/85) Antibody, clone 4F11, Alexa Fluor® 647 Conjugate

100 µL  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Av, R, M, H, B, HtICCMAlexaFluor®647Monoclonal Antibody
      Catalogue Number05-180-AF647
      DescriptionAnti-Cortactin (p80/85) Antibody, clone 4F11, Alexa Fluor® 647 Conjugate
      Alternate Names
      • Src substrate protein p85
      • Cortactin
      • Cortical actin-binding protein
      • p80
      • p80/85
      Background InformationSrc substrate protein p85 (UniProt Q01406; also known as Cortactin, Cortical actin-binding protein, p80, p80/85) is encoded by the CTTN1 (also known as CTTN, EMS1, P85.25) gene (Gene ID 396455) in Gallus gallus (Chicken) species. Cortactin is a cytosolic protein that promotes polymerization and rearrangement of the actin cytoskeleton, especially the actin cortex around the cellular periphery. Cortactin is activated via phosphorylation by upstream kinases in response to extracellular stimuli. Src is known to phosphorylate cortactin on Tyr421, Tyr466, and Tyr482, leading to cortactin interaction with F-actin and Arp2/3. Erk can also phosphorylate cortactin on Ser405 and Ser418, causing recruitment of additional actin nucleation-promoting factors (NPFs), most importantly N-WASP, to the Arp2/3/ complex. Phosphorylated cortactin thus recruits and stabilizes Arp2/3 complex on preexisting F-actin, providing a nucleation site for a new actin branch to form. Cortactin overexpression in tumor cells enhances invasiveness as a result of highly active lamellipodia formation, also known as invadopodia. Cortactin contains an N-terminal aspartic acid-aspartic acid-tryptophan (DDW) sequence (a.a. 29-31 in chicken; a.a. 20-22 in human) that mediates its interaction with Arp2/3, followed by six and a half repeat sequences (a.a. 80-125, 126-162, 163-199, 200-236, 237-273, 274-310, 311-333 in chicken; a.a. 80-116, 117-153, 154-190, 191-227, 228-264, 265-301, 302-324 in human) that mediates F-actin interaction, a proline-rich coiled coil domain (a.a. 349-410 in chicken; a.a. 348-401 in human) that mediates Src SH3 domain interaction, and a C-terminal SH3 domain (a.a. 505-563 in chicken; a.a. 492-550 in human) that contain Erk phosphorylation sites and is important for cortactin interaction with NPFs other than Arp2/3.
      Product Information
      PresentationPurified mouse monoclonal IgG1 antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
      ApplicationAnti-Cortactin (p80/85) Antibody, clone 4F11, Alexa Fluor® 647 Conjugate is an antibody against Cortactin for use in Immunocytochemistry.
      Key Applications
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Cortactin (p80/85) in NIH/3T3 cells.
      The unconjugated antibody (Cat. No. 05-180) is shown to be suitable also for immunocytochemistry, immunofluorescence, immunoprecipitation, and Western blotting applications.
      Biological Information
      ImmunogenMixture of affinity-purified tyrosine phosphoproteins from chick embryo fibroblasts expressing activated pp60src.
      ConcentrationPlease refer to lot specific datasheet.
      SpecificityRecognizes Cortactin.
      Species Reactivity
      • Avian
      • Rat
      • Mouse
      • Human
      • Bovine
      • Hamster
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CTTN1
      • CTTN
      • EMS1
      • P85.25
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~62 kDa observed.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in A431 cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Cortactin (p80/85) in A431 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information