04-220 | Anti-Cyclin B1 (CT) Antibody, clone Y106, rabbit monoclonal

04-220
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFC, ICC, IHC, IH(P), IP, WBRbCulture SupernatantMonoclonal Antibody
      Description
      Catalogue Number04-220
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Cyclin B1 (CT) Antibody, clone Y106, rabbit monoclonal
      Alternate Names
      • DNA polymerase delta auxiliary protein
      • proliferating cell nuclear antigen
      Background InformationCyclins are, as their name suggests, expressed at specific times during the cell cycle, and play a crucial role in the regulation of the cell cycle. Cyclins associate with cyclin-dependent kinases (cdks), and the association is absolutely required for cdk activity. At the conclusion of the relevant phase of the cell cycle, cyclins are rapidly ubiquitinated and degraded, thus returning cdks to their inactive state. Cyclin A associates with cdk2, and together with cyclin E is involved in the G1/S transition. Cyclin A/cdk2 remains active during S-phase. Cyclin B associates with cdk1/cdc2 to regulate the G2/M boundary. The D-cyclins bind cdk4 and cdk6, and result in Rb phosphorylation. The cyclin D1 gene was found in chronic B cell lymphocytic leukemia, and was designated Bcl-1 in a previously used nomenclature.
      References
      Product Information
      FormatCulture Supernatant
      Control
      • HeLa whole cell lysate.
      PresentationUnpurified rabbit monoclonal IgG in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
      Applications
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-Cyclin B1 (C-term) Antibody, clone Y106, rabbit is an antibody against Cyclin B1 (C-term) for use in FC, IC, IH, IH(P), IP & WB.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunohistochemistry: 1:100 dilution from a previous lot was used on immunohistochemistry.

      Immunocytochemistry: 1:50 dilution from a previous lot was used on immunocytochemistry.

      Immunoprecipitation: 1:100 dilution from a previous lot was used on immunoprecipitation.

      Flow Cytometry: 1:100 dilution from a previous lot was used on flow cytometry.
      Biological Information
      ImmunogenSynthetic peptide identical to the C-terminus of human Cyclin B1.
      EpitopeC-terminus
      CloneY106
      HostRabbit
      SpecificityThis antibody recognizes Cyclin B1 at the C-Terminus.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteProven to react with human. Not expected to react with mouse and rat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq]
      Gene Symbol
      • Cyclin
      • MGC8367
      Non-Reactive Species
      • Mouse
      • Rat
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFunction: Essential for the control of the cell cycle at the G2/M (mitosis) transition. Ref.10 Ref.11
      Subunit structure: Interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Binds HEI10. Interacts with catalytically active RALBP1 and CDC2 during mitosis to form an endocytotic complex during interphase. Ref.1 Ref.5 Ref.6
      Subcellular location: Cytoplasm. Nucleus. Centrosome. Ref.8
      Developmental stage: Accumulates steadily during G2 and is abruptly destroyed at mitosis. Ref.1
      Post-translational modification: Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases. Ref.7
      Sequence similarities: Belongs to the cyclin family. Cyclin AB subfamily.

      Molecular Weight58 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on HeLa whole cell lysate.

      Western Blot Analysis:
      A 1:5,000-1:10,000 dilution of this lot was used to detect Cyclin B1 on HeLa whole cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications