05-363 | Anti-Cyclin E Antibody, clone HE12

05-363
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIP, WB, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-363
      Replaces04-223
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Cyclin E Antibody, clone HE12
      Background InformationCyclin E is a regulatory subunit of Cdk2 and controls G1 / S transition during the mammalian cell cycle. Multiple isoforms of Cyclin E are only expressed in tumors but not in normal tissue, suggesting a post transcriptional regulation of Cyclin E. In vitro analyses indicated that these truncated variant isoforms of Cyclin E are able to phosphorylate histone H1. Alterations in the Cyclin E protein have been implicated as indicators of worse prognosis in various cancers.
      References
      Product Information
      FormatPurified
      Control
      • A431 cell lysate
      Presentation0.1M Tris-glycine, pH 7.4, 0.15M NaCl containing 0.05% sodium azide
      Applications
      ApplicationAnti-Cyclin E Antibody, clone HE12 is a high quality Mouse Monoclonal Antibody for the detection of Cyclin E & has been validated in IP, WB, ICC.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Immunocytochemistry
      Biological Information
      ImmunogenRecombinant human cyclin E
      Cloneclone HE12
      HostMouse
      Specificityhuman p52cyclin E
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence information is not yet available.
      Gene Symbol
      • CCNE1
      • CCNE
      Purification MethodProtein G Chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P24864 # Essential for the control of the cell cycle at the G1/S (start) transition.
      SIZE: 410 amino acids; 47077 Da
      SUBUNIT: Interacts with a member of the CDK2/CDK protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Interacts with retinoblastoma binding protein 3 and retinoblastoma-like protein 1. Found in a complex with CDK2, CABLES1 and CCNA1 (By similarity). Part of a complex consisting of UHRF2, CDK2 and CCNE1.
      SUBCELLULAR LOCATION: Nucleus.
      TISSUE SPECIFICITY: Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.
      PTM: Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR.
      SIMILARITY: SwissProt: P24864 ## Belongs to the cyclin family. Cyclin E subfamily.
      Molecular Weight52 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated in immunoblot on human A431 lysate
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C or 6 months at 4°C
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Cyclin E, clone HE12 - 18484 18484
      Anti-Cyclin E, clone HE12 - 18729 18729
      Anti-Cyclin E, clone HE12 - 25307 25307
      Anti-Cyclin E, clone HE12 - 27812 27812
      Anti-Cyclin E, clone HE12 - 31731 31731
      Anti-Cyclin E, clone HE12 - JBC1381938 JBC1381938
      Anti-Cyclin E, clone HE12, human (mouse monoclonal IgG) - 2123699 2123699
      Anti-Cyclin E, clone HE12, human (mouse monoclonal IgG) - 2446889 2446889
      Anti-Cyclin E, clone HE12, human (mouse monoclonal IgG) 2959469
      Anti-Cyclin E, clone HE12, human (mouse monoclonal IgG) 2901512

      References

      Reference overviewApplicationPub Med ID
      Krüppel-like factor 4 regulates genetic stability in mouse embryonic fibroblasts.
      El-Karim, EA; Hagos, EG; Ghaleb, AM; Yu, B; Yang, VW
      Molecular cancer  12  89  2013

      Show Abstract
      Immunofluorescence23919723 23919723
      Differential gene signatures in rat mammary tumors induced by DMBA and those induced by fractionated gamma radiation.
      Hae-June Lee, Yoon-Jin Lee, Chang-Mo Kang, Sangwoo Bae, Dooil Jeoung, Ja-June Jang, Seung-Sook Lee, Chul-Koo Cho, Yun-Sil Lee
      Radiation research  170  579-90  2008

      Show Abstract
      18959458 18959458
      Activation of p53 by nutlin leads to rapid differentiation of human embryonic stem cells.
      Maimets, T; Neganova, I; Armstrong, L; Lako, M
      Oncogene  27  5277-87  2008

      Show Abstract
      Western Blotting18521083 18521083
      IKK alpha regulates estrogen-induced cell cycle progression by modulating E2F1 expression.
      Tu, Z; Prajapati, S; Park, KJ; Kelly, NJ; Yamamoto, Y; Gaynor, RB
      The Journal of biological chemistry  281  6699-706  2006

      Show Abstract
      Fluorescence Activated Cell Sorting (FACS)16407216 16407216
      Stimulation of the Raf/MEK/ERK cascade is necessary and sufficient for activation and Thr-160 phosphorylation of a nuclear-targeted CDK2.
      Lents, Nathan H, et al.
      J. Biol. Chem., 277: 47469-75 (2002)  2002

      Show Abstract
      Immunoprecipitation12359725 12359725
      Cyclin-dependent kinase 2 nucleocytoplasmic translocation is regulated by extracellular regulated kinase.
      Keenan, S M, et al.
      J. Biol. Chem., 276: 22404-9 (2001)  2001

      Show Abstract
      Immunoblotting (Western)11304535 11304535

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies