04-222 | Anti-Cyclin E1 Antibody,clone EP435E rabbit monoclonal

100 µL  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFC, ICC, IP, WBRbCulture SupernatantMonoclonal Antibody
      Catalogue Number04-222
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Cyclin E1 Antibody,clone EP435E rabbit monoclonal
      Alternate Names
      • cyclin E1
      • cyclin Es
      • cyclin Et
      Background InformationCyclins are, as their name suggests, expressed at specific times during the cell cycle, and play a crucial role in the regulation of the cell cycle. Cyclins associate with cyclin-dependent kinases (cdks), and the association is absolutely required for cdk activity. At the conclusion of the relevant phase of the cell cycle, cyclins are rapidly ubiquitinated and degraded, thus returning cdks to their inactive state. Cyclin A associates with cdk2, and together with cyclin E is involved in the G1/S transition. Cyclin A/cdk2 remains active during S-phase. Cyclin B associates with cdk1/cdc2 to regulate the G2/M boundary. The D-cyclins bind cdk4 and cdk6, and result in Rb phosphorylation. The cyclin D1 gene was found in chronic B cell lymphocytic leukemia, and was designated Bcl-1 in a previously used nomenclature.
      Product Information
      FormatCulture Supernatant
      • HeLa whole cell lysate.
      PresentationUnpurified rabbit monoclonal IgG in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
      ApplicationDetect Cyclin E1 using this Anti-Cyclin E1 Antibody, clone EP435E rabbit validated for use in FC, IC, IP & WB.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunocytochemistry: 1:100-250 dilution of a previous lot was used on immunocytochemistry.

      Immunoprecipitation: 1:80 dilution of a previous lot was used on immunoprecipitation.

      Flow Cytometry: 1:20 dilution of a previous lot was used on flow cytometry.
      Biological Information
      ImmunogenSynthetic peptide identical to an internal region of human Cyclin E1.
      SpecificityThis antibody recognizes Cyclin E1.
      Species Reactivity
      • Human
      Species Reactivity NoteProven to react with human. Not expected to react with rat or mouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence information is not yet available. [provided by RefSeq]
      Gene Symbol
      • CCNE
      Non-Reactive Species
      • Mouse
      • Rat
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFunction: Essential for the control of the cell cycle at the G1/S (start) transition.
      Subunit structure: Interacts with a member of the CDK2/CDK protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Interacts with retinoblastoma binding protein 3 and retinoblastoma-like protein 1. Found in a complex with CDK2, CABLES1 and CCNA1 By similarity. Part of a complex consisting of UHRF2, CDK2 and CCNE1.
      Subcellular location: Nucleus.
      Tissue specificity: Highly expressed in testis and placenta. Low levels in bronchial epithelial cells. Ref.10
      Post-translational modification: Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.9 Ref.11 Ref.13 Ref.14
      Sequence similarities: Belongs to the cyclin family. Cyclin E subfamily

      Molecular Weight50 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on HeLa whole cell lysate.

      Western Blot Analysis:
      A 1:1000-1:2000 dilution of this lot was used to detect Cyclin E1 on HeLa whole cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Cyclin E1,clone EP435E - 2151822 2151822
      Anti-Cyclin E1,clone EP435E - 2411804 2411804
      Anti-Cyclin E1,clone EP435E - 2468118 2468118
      Anti-Cyclin E1,clone EP435E - 2043168 2043168
      Anti-Cyclin E1,clone EP435E - 2194033 2194033
      Anti-Cyclin E1,clone EP435E - 2202408 2202408
      Anti-Cyclin E1,clone EP435E - 2345558 2345558
      Anti-Cyclin E1,clone EP435E - NG1580938 NG1580938
      Anti-Cyclin E1,clone EP435E - NG1843046 NG1843046
      Anti-Cyclin E1,clone EP435E -2526853 2526853


      Reference overviewApplicationPub Med ID
      MiR-137 targets estrogen-related receptor alpha and impairs the proliferative and migratory capacity of breast cancer cells.
      Zhao, Y; Li, Y; Lou, G; Zhao, L; Xu, Z; Zhang, Y; He, F
      PloS one  7  e39102  2012

      Show Abstract
      Western Blotting22723937 22723937