04-223 | Anti-Cyclin E2 Antibody, clone EP454Y, rabbit monoclonal

04-223
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIP, WB, ICC, IH(P)RbCulture SupernatantMonoclonal Antibody
      Description
      Catalogue Number04-223
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Cyclin E2 Antibody, clone EP454Y, rabbit monoclonal
      Alternate Names
      • G1/S-specific cyclin E2
      • cyclin E2
      Background InformationCyclins are, as their name suggests, expressed at specific times during the cell cycle, and play a crucial role in the regulation of the cell cycle. Cyclins associate with cyclin-dependent kinases (cdks), and the association is absolutely required for cdk activity. At the conclusion of the relevant phase of the cell cycle, cyclins are rapidly ubiquitinated and degraded, thus returning cdks to their inactive state. Cyclin A associates with cdk2, and together with cyclin E is involved in the G1/S transition. Cyclin A/cdk2 remains active during S-phase. Cyclin B associates with cdk1/cdc2 to regulate the G2/M boundary. The D-cyclins bind cdk4 and cdk6, and result in Rb phosphorylation. The cyclin D1 gene was found in chronic B cell lymphocytic leukemia, and designated Bcl-1, though that nomenclature is no longer used.
      References
      Product Information
      FormatCulture Supernatant
      Control
      • Jurkat cell lysate.
      PresentationUnpurified rabbit monoclonal IgG in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
      Applications
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-Cyclin E2 Antibody, clone EP454Y, rabbit detects level of Cyclin E2 & has been published & validated for use in IP, WB, IC, IH(P).
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry: 1:250 – 500 dilution from a previous lot was used on immunohistochemistry.

      Immunoprecipitation: 1:50 dilution from a previous lot was used on immunocytochemistry.

      Immunocytochemistry: 1:100 – 250 dilution from a previous lot was used on immunocytochemistry.
      Biological Information
      ImmunogenKLH conjugated linear peptide.
      Epitopec-terminus
      CloneEP454Y
      HostRabbit
      SpecificityThis antibody recognizes Cyclin E2.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteProven to react with human. Not expected to react with rat and mouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2. This cyclin has been shown to specifically interact with CIP/KIP family of CDK inhibitors, and plays a role in cell cycle G1/S transition. The expression of this gene peaks at the G1-S phase and exhibits a pattern of tissue specificity distinct from that of cyclin E1. A significantly increased expression level of this gene was observed in tumor-derived cells. [provided by RefSeq]
      Gene Symbol
      • CYCE2
      UniProt Number
      UniProt SummaryFunction: Essential for the control of the cell cycle at the late G1 and early S phase.
      Subunit structure: Interacts with the CDK2 (in vivo) and CDK3 (in vitro) protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex.
      Subcellular location: Nucleus. Ref.3
      Tissue specificity: According to Ref.1: highest levels in adult testis, thymus and brain. Lower levels in placenta, spleen and colon. Consistently elevated levels in tumor-derived cells compared to non-transformed proliferating cells. According to Ref.2: low levels in thymus, prostate, brain, skeletal muscle, and kidney. Elevated levels in lung. According to Ref.3: highly expressed in testis, placenta, thymus and brain. In a lesser extent in small intestine and colon.
      Induction: Activated by papilloma viral oncoproteins E6 and E7 which bind to and inactivate p53 and Rb, respectively.
      Post-translational modification: Phosphorylation by CDK2 triggers its release from CDK2 and degradation via the ubiquitin proteasome pathway By similarity.
      Sequence similarities: Belongs to the cyclin family. Cyclin E subfamily.

      Molecular Weight~47 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot on Jurkat cell lysate.

      Western Blot Analysis: A 1:1,000-1:2,000 dilution of this lot was used to detect Cyclin E2 on Jurkat cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications