04-1043 | Anti-Cytochrome C Antibody, clone EP1326Y, rabbit monoclonal

04-1043
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICC, IH(P), IPRbUnpurifiedMonoclonal Antibody
      Description
      Catalogue Number04-1043
      DescriptionAnti-Cytochrome C Antibody, clone EP1326Y, rabbit monoclonal
      Alternate Names
      • cytochrome c
      • cytochrome c, somatic
      Background InformationCytochrome C is a 15 kDa protein in mitochondria which functions as an electron carrier in the cell respiratory chain by interacting with redox partners of complex III and complex IV. Cytochrome C is highly conserved during evolution. Recent evidence shows that Cytochrome C translocates from mitochondria to the cytosol in the cells undergoing apoptosis and participates in the central control or executioner phase of cell death cascade. Caspase-9 (Apaf-3) and Apaf-1 bind to each other in the presence of Cytochrome C and dATP, an event that leads to Caspase-9 activation. This activation, along with Cytochrome C, plays a major role in the activation of DEVD-specific caspases such as Caspase-3 and DNA fragmentation. Apoptosis inhibitors, Bcl-2 or Bcl-xL, prevent the efflux of Cytochrome C from the mitochondria and Bax, a pro-apoptotic member of the Bcl-2 protein family, can induce Cytochrome C release.
      References
      Product Information
      FormatUnpurified
      Control
      • HeLa, COS, and L929 cell lysates
      PresentationRabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
      Applications
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-Cytochrome C Antibody, clone EP1326Y, Rabbit detects level of Cytochrome C & has been published & validated for use in WB, IC, IH(P) & IP.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      Application NotesImmunohistochemistry Analysis:
      A 1:100 - 250 dilution from a previous lot detected Cytochrome C in human kidney tissue.
      Biological Information
      ImmunogenSynthetic peptide corresponding to residues near the N-terminus of human Cytochrome C.
      EpitopeN-terminus
      CloneEP1326Y
      HostRabbit
      SpecificityThis antibody recognizes Cytochrome C.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteProven to react in human.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes cytochrome c, a component of the electron transport chain in mitochondria. The heme group of cytochrome c accepts electrons from the b-c1 complex and transfers electrons to the cytochrome oxidase complex. Cytochrome c is also involved in initiation of apoptosis. Upon release of cytochrome c to the cytoplasm, the protein binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Many cytochrome c pseudogenes exist, scattered throughout the human genome. [provided by RefSeq].
      Gene Symbol
      • CYC
      • HCS
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.

      Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.

      SUBCELLULAR LOCATION: Mitochondrion matrix.

      PTM: Binds 1 heme group per subunit.

      DISEASE: Defects in CYCS are the cause of thrombocytopenia type 4 (THC4) [MIM:612004]; also known as autosomal dominant thrombocytopenia type 4. Thrombocytopenia is the presence of relatively few platelets in blood. THC4 is a non-syndromic form of thrombocytopenia. Clinical manifestations of thrombocytopenia are absent or mild. THC4 may be caused by dysregulated platelet formation.

      SEQUENCE SIMILARITY: Belongs to the cytochrome c family.

      Molecular Weight~14 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting on HeLa, COS, and L929 cell lysates.

      Western Blotting Analysis:
      1:500 dilution of this antibody was used to detect Cytochrome C in HeLa, COS, and L929 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Cytochrome C, clone EP1326Y 2458985
      Anti-Cytochrome C, clone EP1326Y, Rabbit Monoclonal - 2382224 2382224
      Anti-Cytochrome C, clone EP1326Y, Rabbit Monoclonal - 2390380 2390380
      Anti-Cytochrome C, clone EP1326Y, Rabbit Monoclonal - 2398827 2398827
      Anti-Cytochrome C, clone EP1326Y, Rabbit Monoclonal - 2036710 2036710
      Anti-Cytochrome C, clone EP1326Y, Rabbit Monoclonal - 2287230 2287230
      Anti-Cytochrome C, clone EP1326Y, Rabbit Monoclonal - NG1609573 NG1609573
      Anti-Cytochrome C, clone EP1326Y, Rabbit Monoclonal - NG1644416 NG1644416
      Anti-Cytochrome C, clone EP1326Y, Rabbit Monoclonal - NG1723327 NG1723327
      Anti-Cytochrome C, clone EP1326Y, Rabbit Monoclonal - NG1815320 NG1815320