CBL266 | Anti-Cytokeratin 1 Antibody, 10, clone LH1

CBL266
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberCBL266
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Cytokeratin 1 Antibody, 10, clone LH1
      References
      Product Information
      FormatPurified
      PresentationThe monoclonal is presented at a concentration of 100μg/1ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.
      Applications
      ApplicationDetect Cytokeratin 1 using this Anti-Cytokeratin 1 Antibody, 10, clone LH1 validated for use in WB, IH.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesWestern blotting.

      IHC formalin fixed sections, HIER (Microwave citrate buffer pH6.0) produces some staining but pretreatment with type XXIV protease (0.5mg/ml) produces best results.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenKeratin 1 and 10.
      CloneLH1
      HostMouse
      SpecificityThis antibody reacts with the suprabasal cells of stratified squamous epithelia in human, murine and porcine species.
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21.
      Gene Symbol
      • KRT1
      • CK-1
      • EHK1
      • Keratin-1
      • Cytokeratin-1
      • CK1
      • KRT1A
      • K1
      • KRTA
      UniProt Number
      UniProt SummarySIZE: 644 amino acids; 66018 Da
      SUBUNIT: Heterotetramer of two type I and two type II keratins. keratin-1 is generally associated with keratin-10.
      TISSUE SPECIFICITY: The source of this protein is neonatal foreskin. The 67-kDa type II keratins are expressed in terminally differentiating epidermis.
      PTM: Undergoes deimination of some arginine residues (citrullination).
      DISEASE: SwissProt: P04264 # Defects in KRT1 are a cause of epidermolytic hyperkeratosis (EHK) [MIM:113800]; also known as bullous congenital ichthyosiform erythroderma (BIE). EHK is a hereditary skin disorder characterized by blistering and a marked thickening of the stratum corneum. & Defects in KRT1 are the cause of Curth-Macklin type ichthyosis hystrix (IHCM) [MIM:146590]. & Defects in KRT1 are a cause of nonepidermolytic palmoplantar keratoderma (NEPPK) [MIM:600962]; also known as Unna- Thost disease. & Defects in KRT1 are a cause of annular epidermolytic ichthyosis (AEI) [MIM:607602]; also known as cyclic ichthyosis with epidermolytic hyperkeratosis. AEI resembles clinical and histologic features of both epidermolytic hyperkeratosis and ichthyosis bullosa of Siemens. & Defects in KRT1 are the cause of keratosis palmoplantaris striata III (PPKS3) [MIM:607654]; also known as striate palmoplantar keratoderma III (SPPK3). PPKS3 is an autosomal dominant disorder affecting palm and sole skin where stratum corneum and epidermal layers are thickened. There is no involvement of non-palmoplantar skin, and both hair and nails are normal.
      SIMILARITY: SwissProt: P04264 ## Belongs to the intermediate filament family.
      MISCELLANEOUS: There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsFor use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-HUMAN CYTOKERATIN 1/10 MONOCLONAL ANTIBODY - 2398149 2398149
      MOUSE ANTI-HUMAN CYTOKERATIN 1/10 MONOCLONAL ANTIBODY - 2063159 2063159
      MOUSE ANTI-HUMAN CYTOKERATIN 1/10 MONOCLONAL ANTIBODY - 2201427 2201427
      MOUSE ANTI-HUMAN CYTOKERATIN 1/10 MONOCLONAL ANTIBODY -2533220 2533220
      MOUSE ANTI-HUMAN CYTOKERATIN 1/10 MONOCLONAL ANTIBODY -2608160 2608160
      MOUSE ANTI-HUMAN CYTOKERATIN 1/10 MONOCLONAL ANTIBODY -2770798 2770798
      MOUSE ANTI-HUMAN CYTOKERATIN 1/10 MONOCLONAL ANTIBODY -2809271 2809271

      References

      Reference overviewPub Med ID
      In vitro culture of Keratinocytes from human umbilical cord blood mesenchymal stem cells: the Saigonese culture.
      Cong Toai Tran,Duy Thao Huynh,Ciro Gargiulo,Phuong Thao Nguyen,Thi Thanh Thuy Tran,Minh Tuan Huynh,Thanh Tung Nguyen,Luis Filgueira,D Micheal Strong
      Cell and tissue banking  12  2011

      Show Abstract
      20349146 20349146
      Expression of simple epithelial keratins 8 and 18 in epidermal neoplasia.
      Markey, A C, et al.
      J. Invest. Dermatol., 97: 763-70 (1991)  1991

      Show Abstract
      1717607 1717607
      An investigation of cytokeratin expression in skin epithelial cysts and some uncommon types of cystic tumours using chain-specific antibodies.
      Broekaert, D, et al.
      Arch. Dermatol. Res., 282: 383-91 (1990)  1990

      Show Abstract
      1701984 1701984

      Data Sheet

      Title
      MOUSE ANTI-HUMAN CYTOKERATIN 1/10 MONOCLONAL ANTIBODY